The existence of lymphatic vessels in the dental pulp has been a matter of continuing controversy. We have now used light microscopy to examine semithin transverse sections of perfusion-fixed incisors and canines in cats. Lymphatics were found in all the teeth studied. In most teeth they were present in the coronal, middle, and apical regions of the pulp; but in a few they were lacking coronally and in the middle. Within individual teeth, lymphatics were found in the subodontoblastic zone or more centrally in the pulp; but none were found in the odontoblast layer or in the pulp horns. Vessels located by light microscopy were subsequently examined by transmission electron microscopy. Their ultrastructural features were typical of lymphatics and included irregular, attenuated endothelium with adjacent cells joined in different ways. Occasional gaps connected the extracellular spaces with their lumens, and abluminal endothelial projections appeared to form open end bulbs. There was very little basement membrane, but anchoring filaments were found near the abluminal surface of the endothelium and near collagen fibrils. The total cross-sectional area of lymphatic vessels was measured in semithin sections and, with pulp area, increased from the coronal region to the middle. However, both areas decreased from the middle to the apical region suggesting either that lymph flows faster as it reaches the foramens of the apical delta or that some vessels leave the tooth through lateral root canals. Using the methods of light and transmission electron microscopy, therefore, we have shown that pulp lymphatic vessels exist. Questions remain, however, about their distribution within teeth, variations between teeth, and routes of exit from teeth.

The collagenous fibers of von Korff pass from the dentin matrix between the odontoblasts into the dental pulp. Although collagen fibrils are known to be present between odontoblasts, the existence of von Korff fibers has remained controversial. This may be because their continuity between the dentin matrix and the pulp has not been demonstrated ultrastructurally. In this study we have examined the odontoblast layer in the middle to apical regions of perfusion-fixed permanent canine teeth of cats by using transmission electron microscopy. Ultrathin sections of demineralized specimens revealed frequent bundles of collagen fibrils 1) entering the odontoblast layer from the predentin, 2) present between odontoblast cell bodies, and 3) passing from between the odontoblasts into the pulp. The question of continuity of these bundles from the predentin, across the odontoblast layer into the pulp was examined in ultrathin serial sections. Unbroken continuity of a collagen bundle from the predentin between the odontoblasts into the pulp was established in a reconstruction of one series of 22 serial sections and was very strongly suggested by a number of other series in which the numbers of available sections restricted their full visibility. This investigation has shown, therefore, that classical von Korff fibers are present and that these fibers are present in fully erupted teeth with closed apices, i.e., at a time when secondary circumpulpal dentinogenesis is in progress. The findings call for a reexamination of the question of von Korff fibers during mantle dentinogenesis and primary circumpulpal dentinogenesis. Resolution of their existence at the earlier stages of dentinogenesis should be possible by using the ultrathin serial-sectioning technique.

Aim: Perforation peritonitis is the most common surgical emergency encountered by surgeons in India. The etiology and sites of perforation shows wide geographical variation. The objective of the study was to find the spectrum of perforation peritonitis & highlight its management at Maharishi Markendeshwar Institute of Medical Sciences & Research, Mullana (MMIMSR).Methods: 93 Operated patients of perforation peritonitis were studied retrospectively in terms of clinical presentation, duration, operative findings and postoperative morbidity and mortality over a period of two years between 2011 to 2013 at MMIMSR Mullana. All the patients had undergone emergency laparatomy under general anesthesia and sites of perforation were identified & managed.Results: The most common cause of perforation peritonititis noticed in our series was peptic ulcer perforation 43 cases (46%), followed by ileal perforation 30 cases (32%), appendicular perforation 6 cases (6.4%), gallbladder perforation 5 cases (5.3%) and all the jejunal perforation 6cases (6.4%) was post traumatic. Large bowel and malignant perforation were least common in our series. Highest no. of perforation noticed in upper part of Gastro intestinal tract as compared to western countries where perforations are seen in distal parts. Mortality was of 11 cases (11.8%) & morbidity was noticed in 55 cases (59%).Conclusion: Peptic ulcer perforation peritonitis is the leading etiology. Mortality is comparable to that of best centre. Aggressive resuscitation and early minimum surgery are required to avoid the high morbidity and mortality. Major complication noticed was wound infection and dehiscence.Bangladesh Journal of Medical Science Vol.15(1) 2016 p.70-73

Abstract:Background: W.H.O has introduced a surgical safety checklist as part of its Safe Surgery Saves Lives initiative. The checklist's purpose was to reduce surgical complications that resulted from inadequate safety practices and promote greater communication among surgery teams. It is made necessary to use the surgical safety checklist in the operation theatres across the world but is it really used in the actual practical setup? Material & Methods: With that idea in mind we had conducted a study to know about the awareness and practical use of surgical safety checklist among surgeons attending Haryana Chapter of Association of Surgeons in India Conference (HASICON) 2014 in there surgical practice. Printed performa with a set of questions was given to the delegates and the result was analysed. Result: Only 57% of the study group had heard about the safety checklist and only 32% of them are using it in their surgical practice. Conclusion:There is an urgent need to spread awareness about the safety checklist and one way of achieving it is by demonstration papers in conferences across the world.

Abstract:Aim: To evaluate the usefulness and feasibility of the Alvarado scoring system in acute surgery in reducing the percentage of negative appendicectomy in our institute. Materials and Methods: A prospective study was conducted, comprising 100 patients, admitted to department of Surgery in Maharishi Markendeshwar Institute of Medical sciences & Research (M.M.I.M.S.R), Mullana, Ambala, India during the period October 2012 to July 2014 with a preliminary diagnosis of acute appendicitis. Patients of both sexes and all age groups were included in the study and their Alvarado scores calculated, on the basis of which patients were divided into three groups: group1 (alv. Score >7) , group 2 (alv. score 5-6), group 3 (alv. Score <4). The signs, symptoms, laboratory values, surgical interventions, and pathology reports of each patient were evaluated. Diagnosis was confirmed by histopathological examination. Sensitivity, specificity, and positive and negative predictive values of scoring system were calculated. Results: Out of 100 cases (82 males, 18 females), 76 Patients belonged to be in group1 (76%), 20 patients were in group 2 (20%) and 4 patients were in group 3 (4%). All 100 patients were operated, of which 86 patients found to have inflamed appendix (86%). 82 (82%) of operated patients were male and 18 (18%) were females. Out of 82 of operated males, 64 were having score > 7, 8 were having score 5-6 and 1 had score <4. Out of 18 operated females, 6 were having score > 7, 4 were having score 5-6, and 2 females had scoring <4. Negative appendectomy rate in our study was 14% which showed that Alvarado score helped in making diagnosis of acute appendicitis; thus reducing negative appendicectomy. Sensitivity and specificity of the Alvarado scoring system were found to be 83.7% and 71% respectively. Positive and negative predictive values were 94.7% and 83.3% respectively. Conclusion: Alvarado score can be used effectively in acute surgery in our setup to reduce the incidence of negative appendectomies in both male and females. It can be applied easily for acute surgery even by junior surgical colleagues with limited diagnostic facilities available to them.ry even by junior surgical colleagues with limited diagnostic facilities available to them