The presence of ferric and ferrous iron in the nonheme iron store of resident macrophages in different tissues and organs: histochemical demonstrations by the perfusion-Perls and -Turnbull methods in the rat

Meguro, Reiko; Asano, Yoshiya; Odagiri, S; Li, Chengtai; Iwatsuki, Hiroyasu; Shoumura, Kazuhiko

doi:10.1679/aohc.68.171

Cited by 30 publications

(27 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1E). We identified no spinning cells in the spleen indicating that the iron-rich macrophages (which are abundant in this tissue) do not spin (29). To ascertain whether the observed spinning cells comprised a single morphological class, we analyzed their appearance using both transmitted light and fluorescent imaging staining with the membrane marker FM1-43 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

No evidence for intracellular magnetite in putative vertebrate magnetoreceptors identified by magnetic screening

Edelman

Fritz

Nimpf

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The cellular basis of the magnetic sense remains an unsolved scientific mystery. One theory that aims to explain how animals detect the magnetic field is the magnetite hypothesis. It argues that intracellular crystals of the iron oxide magnetite (Fe3O4) are coupled to mechanosensitive channels that elicit neuronal activity in specialized sensory cells. Attempts to find these primary sensors have largely relied on the Prussian Blue stain that labels cells rich in ferric iron. This method has proved problematic as it has led investigators to conflate iron-rich macrophages with magnetoreceptors. An alternative approach developed by Eder et al. [Eder SH, et al. (2012) Proc Natl Acad Sci USA 109(30):12022–12027] is to identify candidate magnetoreceptive cells based on their magnetic moment. Here, we explore the utility of this method by undertaking a screen for magnetic cells in the pigeon. We report the identification of a small number of cells (1 in 476,000) with large magnetic moments (8–106 fAm2) from various tissues. The development of single-cell correlative light and electron microscopy (CLEM) coupled with electron energy loss spectroscopy (EELS) and energy-filtered transmission electron microscopy (EFTEM) permitted subcellular analysis of magnetic cells. This revealed the presence of extracellular structures composed of iron, titanium, and chromium accounting for the magnetic properties of these cells. Application of single-cell CLEM to magnetic cells from the trout failed to identify any intracellular structures consistent with biogenically derived magnetite. Our work illustrates the need for new methods to test the magnetite hypothesis of magnetosensation.

show abstract

Section: Resultsmentioning

confidence: 99%

No evidence for intracellular magnetite in putative vertebrate magnetoreceptors identified by magnetic screening

Edelman

Fritz

Nimpf

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Wild-type and sla mice were perfused with the perfusion-Perl's method, and spinal cords were prepared for electron microscopy according to a previously described protocol (Meguro et al, 2005). In brief, mice were first perfused with 0.1 M phosphate buffer, pH 7.4, containing heparin (10 U/ml), then with 4% paraformaldehyde and 1.5% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4, followed by 4% paraformaldehyde, 1% glutaraldehyde, and 1% potassium ferrocyanide in distilled H 2 O, pH 1.0.…”

Section: Methodsmentioning

confidence: 99%

Iron Efflux from Oligodendrocytes Is Differentially Regulated in Gray and White Matter

Schulz¹,

Vulpe²,

Harris³

et al. 2011

J. Neurosci.

View full text Add to dashboard Cite

Accumulation of iron occurs in the CNS in several neurodegenerative diseases. Iron is essential for life but also has the ability to generate toxic free radicals if not properly handled. Iron homeostasis at the cellular level is therefore important to maintain proper cellular function, and its dysregulation can contribute to neurodegenerative diseases. Iron export, a key mechanism to maintain proper levels in cells, occurs via ferroportin, a ubiquitously expressed transmembrane protein that partners with a ferroxidase. A membrane-bound form of the ferroxidase ceruloplasmin is expressed by astrocytes in the CNS and regulates iron efflux. We now show that oligodendrocytes use another ferroxidase, called hephaestin, which was first identified in enterocytes in the gut. Mice with mutations in the hephaestin gene (sex-linked anemia mice) show iron accumulation in oligodendrocytes in the gray matter, but not in the white matter, and exhibit motor deficits. This was accompanied by a marked reduction in the levels of the paranodal proteins contactin-associated protein 1 (Caspr) and reticulon-4 (Nogo A). We show that the sparing of iron accumulation in white matter oligodendrocytes in sex-linked anemia mice is due to compensatory upregulation of ceruloplasmin in these cells. This was further confirmed in ceruloplasmin/hephaestin double-mutant mice, which show iron accumulation in both gray and white matter oligodendrocytes. These data indicate that gray and white matter oligodendrocytes can use different iron efflux mechanisms to maintain iron homeostasis. Dysregulation of such efflux mechanisms leads to iron accumulation in the CNS.

show abstract

“…The DAB-enhanced histochemical iron stains described by Smith (Smith et al 1997) and LeVine (LeVine 1991) have been reported to label AD plaques. The Meguro method (Meguro et al 2007) has not been described in human brain tissue or animal models of AD but has been widely used in other organs in rats, mice, and monkeys (Meguro et al 2005;Meguro et al 2007;Freret et al 2008;Iwatsuki et al 2008;Meguro et al 2008;Butt et al 2010;Winter et al 2010;Butt et al 2011;Shpyleva et al 2011).…”

Section: +mentioning

confidence: 99%

Comparison of Histological Techniques to Visualize Iron in Paraffin-embedded Brain Tissue of Patients with Alzheimer’s Disease

Duijn

Nabuurs

Duinen

et al. 2013

J Histochem Cytochem.

View full text Add to dashboard Cite

SummaryBetter knowledge of the distribution of iron in the brains of Alzheimer's disease (AD) patients may facilitate the development of an in vivo magnetic resonance (MR) marker for AD and may cast light on the role of this potentially toxic molecule in the pathogenesis of AD. Several histological iron staining techniques have been used in the past but they have not been systematically tested for sensitivity and specificity. This article compares three histochemical techniques and ferritin immunohistochemistry to visualize iron in paraffin-embedded human AD brain tissue. The specificity of the histochemical techniques was tested by staining sections after iron extraction. Iron was demonstrated in the white matter, in layers IV/V of the frontal neocortex, in iron containing plaques, and in microglia. In our hands, these structures were best visualized using the Meguro iron stain, a method that has not been described for iron staining in human brain or AD in particular. Ferritin immunohistochemistry stained microglia and iron containing plaques similar to the Meguro method but was less intense in myelin-associated iron. The Meguro method is most suitable for identifying iron-positive structures in paraffinembedded human AD brain tissue. (J Histochem Cytochem 61:785-792, 2013)

show abstract

The presence of ferric and ferrous iron in the nonheme iron store of resident macrophages in different tissues and organs: histochemical demonstrations by the perfusion-Perls and -Turnbull methods in the rat

Cited by 30 publications

References 28 publications

No evidence for intracellular magnetite in putative vertebrate magnetoreceptors identified by magnetic screening

No evidence for intracellular magnetite in putative vertebrate magnetoreceptors identified by magnetic screening

Iron Efflux from Oligodendrocytes Is Differentially Regulated in Gray and White Matter

Comparison of Histological Techniques to Visualize Iron in Paraffin-embedded Brain Tissue of Patients with Alzheimer’s Disease

Contact Info

Product

Resources

About