The presence of blood group and lymphocyte antigens on porcine granulocytes

Abstract

Suspensions of highly viable (< 95 To) granulocytes minimally contaminated by other cell types were isolated from the peripheral blood of pigs by a single centrifugation with low molecular weight dextran and after preferential lysis of erythrocytes by hypotonic shock. A complement-dependent cytotoxic test showed the presence of antigens of the SLA major histocompatibility complex, the SLB leucocyte system and the A and E blood group systems on the granulocytes. Some SLA typing reagents against class I (SD) antigens did not react with granulocytes, however, or yielded dubious reactions. The findings showed that the reactivity of SLA sera resembles the reactivity of human HLA sera. The results also show that compatibility in the SLA, SLB, A and E systems will have to be taken into account when preparing alloirnrnune sera for the determination of granulocyte-specific antigens of pigs.

Detection of a new non‐MHC alloantigenic system (SLD) in pigs

“…Homogeneity of these suspensions was 70-90 %. Polymorphonuclear cells were isolated from peripheral blood after centrifugation with low molecular weight dextran (Klaudy et al, 1981). Thrombocytes were obtained from Na,-EDTA blood by simple differential centrifugation (Colombani et al, 1970).…”