The presence of an insulin-like androgenic gland factor (IAG) and insulin-like peptide binding protein (ILPBP) in the ovary of the blue crab, Callinectes sapidus and their roles in ovarian development

Huang, Xiaoshuai; Ye, Haihui; Chung, J. Sook

doi:10.1016/j.ygcen.2017.05.001

Cited by 22 publications

(21 citation statements)

References 35 publications

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“…Our group previously suggested that the IAG gene is present in both male and female genomes, but is exclusively expressed in the male AG [ 5 ]. However, on the basis of studies in two species of crab ( Callinectes sapidus and Scylla paramamosain ) showing that the IAG gene is also expressed in females, it has recently been postulated that IAG is also involved in the processes of growth and reproduction in female crustaceans [ 12 , 13 ]. Although the above two studies did not detect the peptide or present clear proof of function, the premise that was put forward is supported by the findings in the current study of IAG expression in the muscle tissues of both P .…”

Section: Discussionmentioning

confidence: 99%

“…Since the IAG gene is known as a growth and reproduction controlling factor [ 5 , 12 , 13 , 23 ], somatic and reproductive related tissues were dissected from three P . fallax males, six marbled crayfish and one P .…”

Section: Methodsmentioning

confidence: 99%

“…Although the IAG gene has been found in the genome of both genders in gonochoristic species, it was originally believed to be expressed exclusively in the male AG. However, a few recent studies have suggested that, despite its prominent role as a masculinization-inducing hormone, IAG also stimulates growth and ovarian development in females [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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The gene encoding the insulin-like androgenic gland hormone in an all-female parthenogenetic crayfish

et al. 2017

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Male sexual differentiation in crustaceans is controlled by the androgenic gland (AG), a unique male endocrine organ that, in decapods, is located at the base of the 5th pereiopod. In these animals, the insulin-like androgenic gland hormone (IAG) is the major factor secreted from the AG to induce masculinization and maintain male characteristics. It has, however, recently been proposed that this hormone also plays a role in growth and ovarian development in females. In this study, we tested such a possibility by searching for the IAG gene in the marbled crayfish, a parthenogenetic animal that reproduces asexually to form an all-female genetic clone. Based on the phylogenetic relationship between the marbled crayfish and Procambarus fallax, a gonochoristic species of the same North American Cambaridae family, we searched for the IAG gene in the marbled crayfish and then fully sequenced it. The open reading frame of the gene was found to be completely identical in the two species, and their introns shared over 94% identity. It was also found that, in addition to its expression at the base of the 5th pereiopod and in the testes of male P. fallax crayfish, IAG was expressed in the muscle tissue of P. fallax males and females and even of the parthenogenetic marbled crayfish. These findings provide new insight into possible functions of IAG, in addition to its role as a masculinization-inducing factor, and also constitute the basis for a discussion of the evolutionary relationship between the above two species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The gene encoding the insulin-like androgenic gland hormone in an all-female parthenogenetic crayfish

et al. 2017

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“…While IAG is a very well-established sexual differentiating factor, leading to masculinization across malacostracans [ 23 ], its effect is not strictly conserved across the diverse decapods. In several cases, it has been shown that IAG is not expressed strictly in males or the AG [ 56 ]. In the blue swimmer crab, Callinectes sapidus , IAG expression was observed in the ovary [ 56 ] and hepatopancreas [ 57 ], suggesting involvement in ovarian development and carbohydrate metabolism.…”

Section: Resultsmentioning

confidence: 99%

Multi-Tissue Transcriptome Analysis Identifies Key Sexual Development-Related Genes of the Ornate Spiny Lobster (Panulirus ornatus)

Ventura

Chandler

Nguyen

et al. 2020

Genes

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Sexual development involves the successive and overlapping processes of sex determination, sexual differentiation, and ultimately sexual maturation, enabling animals to reproduce. This provides a mechanism for enriched genetic variation which enables populations to withstand ever-changing environments, selecting for adapted individuals and driving speciation. The molecular mechanisms of sexual development display a bewildering diversity, even in closely related taxa. Many sex determination mechanisms across animals include the key family of “doublesex- and male abnormal3-related transcription factors” (Dmrts). In a few exceptional species, a single Dmrt residing on a sex chromosome acts as the master sex regulator. In this study, we provide compelling evidence for this model of sex determination in the ornate spiny lobster Panulius ornatus, concurrent with recent reports in the eastern spiny lobster Sagmariasus verreauxi. Using a multi-tissue transcriptomic database established for P. ornatus, we screened for the key factors associated with sexual development (by homology search and using previous knowledge of these factors from related species), providing an in-depth understanding of sexual development in decapods. Further research has the potential to close significant gaps in our understanding of reproductive development in this ecologically and commercially significant order.

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“…In the blue crab, Callinectes sapidus, in vivo silencing of hepatopancreas source IAG resulted in higher levels of hemolymph glucose than the control group, accompanied by significantly lower amounts of carbohydrate in the hepatopancreas, indicating a function of IAG in carbohydrate metabolism ( 4 ). Additionally, the IAG transcripts were also found in the ovary of C. sapidus and S. paramamosain , therefore being deduced as a regulator in ovarian development ( 5 , 18 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of the Insulin-Like Androgenic Gland Hormone in the Swimming Crab, Portunus trituberculatus, and Its Involvement in the Insulin Signaling System

Jiang

Zheng

et al. 2020

Front. Endocrinol.

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The insulin-like androgenic gland hormone (IAG) is mainly produced in the androgenic gland (AG) of the male crustaceans and is a crucial regulator in male sexual differentiation. In the current study, the full-length cDNA of IAG in the swimming crab, Portunus trituberculatus ( Pt-IAG ), was cloned and characterized. Similar to other reported IAGs, the deduced amino acid sequence of Pt-IAG consists of signal peptide, B chain, C peptide, and A chain, containing six conserved cysteines that form two interchain disulfide bonds and one intra-B chain disulfide bond. Tissue distribution analysis suggested that the Pt-IAG cDNA was highly expressed in the AG and was slightly expressed in several other tissues. A short-term silencing of PtIAG with double-stranded RNA was found to reduce the transcript levels of insulin receptor (Pt-IR) and insulin-like growth factor-binding protein (Pt-IGFBP), suggesting the Pt-IAG might perform its biological function through the insulin family-based signaling system. Bilateral eyestalk ablation (ESA) induced the expression of Pt-IAG in the AG at 4 and 7 days after surgery, while the transcript levels of Pt-IR in the AG and testis and Pt-IGFBP in the muscle, testis, and thoracalia ganglia were significantly decreased from 1 day after surgery. The results suggested that the Pt-IR and Pt-IGFBP might also be the targets of eyestalk neuropeptides and responded to the ESA independent of IAG regulation.

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The presence of an insulin-like androgenic gland factor (IAG) and insulin-like peptide binding protein (ILPBP) in the ovary of the blue crab, Callinectes sapidus and their roles in ovarian development

Cited by 22 publications

References 35 publications

The gene encoding the insulin-like androgenic gland hormone in an all-female parthenogenetic crayfish

The gene encoding the insulin-like androgenic gland hormone in an all-female parthenogenetic crayfish

Multi-Tissue Transcriptome Analysis Identifies Key Sexual Development-Related Genes of the Ornate Spiny Lobster (Panulirus ornatus)

Molecular Characterization of the Insulin-Like Androgenic Gland Hormone in the Swimming Crab, Portunus trituberculatus, and Its Involvement in the Insulin Signaling System

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