Molecular Characterization of the Insulin-Like Androgenic Gland Hormone in the Swimming Crab, Portunus trituberculatus, and Its Involvement in the Insulin Signaling System

Jiang, Qinghua; Zheng, Hongkun; Zheng, Liang; Wang, Yaojin; Wang, Mengen; Xie, Xuewen; Zhu, Dongfa

doi:10.3389/fendo.2020.00585

Cited by 16 publications

(6 citation statements)

References 22 publications

(32 reference statements)

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“…ILPs have been identified in decapod crustacean transcriptomes (28,32,33,38,83,(110)(111)(112)(113)(114). One of the best characterized ILP is the insulin-like androgenic gland hormone (IAG); it is expressed primarily in the androgenic gland and determines adult male characteristics (42,83,108,109,115,116). In Portunus trituberculatus, Pt-IAG is expressed at very low levels in the YO (116).…”

Section: Insulin-like Peptide Receptor Signalingmentioning

confidence: 99%

“…One of the best characterized ILP is the insulin-like androgenic gland hormone (IAG); it is expressed primarily in the androgenic gland and determines adult male characteristics ( 42 , 83 , 108 , 109 , 115 , 116 ). In Portunus trituberculatus , Pt-IAG is expressed at very low levels in the YO ( 116 ). Other crustacean ILPs are expressed in most tissues, but at differing levels.…”

Section: Receptor Tyrosine Kinasesmentioning

confidence: 99%

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Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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A pair of Y-organs (YOs) are the molting glands of decapod crustaceans. They synthesize and secrete steroid molting hormones (ecdysteroids) and their activity is controlled by external and internal signals. The YO transitions through four physiological states over the molt cycle, which are mediated by molt-inhibiting hormone (MIH; basal state), mechanistic Target of Rapamycin Complex 1 (mTORC1; activated state), Transforming Growth Factor-β (TGFβ)/Activin (committed state), and ecdysteroid (repressed state) signaling pathways. MIH, produced in the eyestalk X-organ/sinus gland complex, inhibits the synthesis of ecdysteroids. A model for MIH signaling is organized into a cAMP/Ca2+-dependent triggering phase and a nitric oxide/cGMP-dependent summation phase, which maintains the YO in the basal state during intermolt. A reduction in MIH release triggers YO activation, which requires mTORC1-dependent protein synthesis, followed by mTORC1-dependent gene expression. TGFβ/Activin signaling is required for YO commitment in mid-premolt. The YO transcriptome has 878 unique contigs assigned to 23 KEGG signaling pathways, 478 of which are differentially expressed over the molt cycle. Ninety-nine contigs encode G protein-coupled receptors (GPCRs), 65 of which bind a variety of neuropeptides and biogenic amines. Among these are putative receptors for MIH/crustacean hyperglycemic hormone neuropeptides, corazonin, relaxin, serotonin, octopamine, dopamine, allatostatins, Bursicon, ecdysis-triggering hormone (ETH), CCHamide, FMRFamide, and proctolin. Contigs encoding receptor tyrosine kinase insulin-like receptor, epidermal growth factor (EGF) receptor, and fibroblast growth factor (FGF) receptor and ligands EGF and FGF suggest that the YO is positively regulated by insulin-like peptides and growth factors. Future research should focus on the interactions of signaling pathways that integrate physiological status with environmental cues for molt control.

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Section: Insulin-like Peptide Receptor Signalingmentioning

confidence: 99%

Section: Receptor Tyrosine Kinasesmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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“…The IAG in M. rosenbergii is exclusively expressed only in AG rather than in the other tissues Ma et al, 2013). While in other crustaceans including shrimps, crayfish, and crabs (Mareddy et al, 2011;Li et al, 2012;Zhang et al, 2014;Shi L. et al, 2019;Jiang et al, 2020c) the IAG is specifically expressed in the AG, it also expressed in other tissues. Therefore, IAG may is not the only key regulator of sexual differentiation (Farhadi et al, 2021a).…”

Section: Introductionmentioning

confidence: 95%

A Novel Imprinted Gene (Sp-Pol) With Sex-Specific SNP Locus and Sex-Biased Expression Pattern Provides Insights Into the Gonad Development of Mud Crab (Scylla paramamosain)

et al. 2021

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Identification and exploring the role of novel sex-related genes is a crucial step for understanding the regulation mechanism of sexual development. In the present study, we identified a novel sex-related gene (designated as Sp-Pol) upstream of a sex-specific single nucleotide polymorphism (SNP). SNP1888 together with Sp-Pol were mapped on LG32 (which is a sex-related linkage group) of a high-density genetic map. The full-length cDNA of Sp-Pol consists of 1703 bp with an open reading frame (ORF) encoding 204 amino acids, a 344 bp 5′-UTR and a 744 bp 3′-UTR. Phylogenetic analysis showed that Sp-Pol may need to be classified as a new gene family due to the very low sequence identity with other known genes (less than 25% identity). The sex-biased expression pattern of Sp-Pol starts from crablet stage V (C5) with approximately three- to four-fold higher in males than in females. Sp-Pol was expressed at a higher level in gonads compared to other tissues, with the highest expression level in the testis. In testis, a downward trend was observed in the expression level of Sp-Pol from the testis stage I (T1) to testis stage III (T3). After unilateral eyestalk ablation, the expression level of Sp-Pol significantly increased in testis and hepatopancreas in males, while it was downregulated in the hepatopancreas of females. Fluorescence in situ hybridization (FISH) assay revealed that Sp-Pol transcripts were strongly localized in the epithelia of seminiferous tubules of the testis, and in the ovary, it was detected in the oogonium cells. These findings showed that Sp-Pol may play crucial roles in the gonad development of S. paramamosain.

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“…An insulin family-based signaling system has been proposed based on the structure similarity of IAG to the insulin/IGF family (Sharabi et al, 2016). Several peptide receptors including tyrosine kinase insulin receptor (TKIR), G-protein-coupled receptor (GPCR), insulin receptor (IR), and insulin-like growth factorbinding peptide (IGFBP) have been identified to be the receptor for IAG (Rosen et al, 2010;Aizen et al, 2016;Chandler et al, 2017;Jiang et al, 2020c;Ventura et al, 2020).…”

Section: Downstream Mechanisms Of Sex In Male Decapodsmentioning

confidence: 99%

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

et al. 2021

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Crustacean culture has been developing rapidly in various parts of the world. Therefore, it is important to understand their reproductive biology. Insulin-like androgenic gland hormone (IAG) secreted from the androgenic gland (AG) is widely accepted as a key regulator of sexual differentiation in male crustaceans. However, recently several sex-related genes (i.e., CFSH, DEAD-box family, Tra-2, Sxl, Dsx, Fem-1, Sox gene family, Foxl2, and Dmrt gene family) have been identified via transcriptomic analysis in crustaceans, indicating that sexual differentiation in crustaceans is more complicated than previously expected. It has been found that several non-coding RNAs (i.e., miRNAs, lncRNAs, and piRNAs) and IAG receptors may be involved in the sexual development of decapods. Identification and study of the regulation mechanism of sex-related genes, non-coding RNAs, and IAG receptors will provide valuable information regarding sexual development in decapods. In this review, the roles of hormonal and genetic factors in both males and females are discussed. In males, crustacean female sex hormone (CFSH), Sxl, Dmrt gene family, Dsx, Sox gene family, GEM, Fem-1, l-GnRH-III, and corazonin play important roles in IAG regulation in the “eyestalk-IAG-testis” endocrine axis. Unlike males, the regulation mechanism and interaction of sexual genes are relatively unknown in females. However, CFSH, IAG, Fem-1, FAMeT, Slo, UCHLs, Erk2, Cdc2, EGFR, Vg, VgR, and VIH seem to play crucial roles during ovarian development. This study summarizes the available information in the field, highlights gaps, and lays the foundations for further studies and a better understanding of the regulatory mechanism of sexual development in decapods.

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Molecular Characterization of the Insulin-Like Androgenic Gland Hormone in the Swimming Crab, Portunus trituberculatus, and Its Involvement in the Insulin Signaling System

Cited by 16 publications

References 22 publications

Signaling Pathways That Regulate the Crustacean Molting Gland

Signaling Pathways That Regulate the Crustacean Molting Gland

A Novel Imprinted Gene (Sp-Pol) With Sex-Specific SNP Locus and Sex-Biased Expression Pattern Provides Insights Into the Gonad Development of Mud Crab (Scylla paramamosain)

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

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