The presence of a deletion sequence in the BHV-1 UL49 homolog in a live attenuated vaccine for infectious bovine rhinotracheitis (IBR)

Kamiyoshi, Takeshi; Murakami, Kenji; Konishi, Misako; Izumi, Yasuhiro; Sentsui, Hiroshi

doi:10.1016/j.vaccine.2007.11.037

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…BHV-1 subtypes 1.1 and 1.2 are very closely related and are classified based on specific symptoms in infected cattle (Muylkens et al 2007). Molecular methods, including enzymatic restriction fragment length polymorphism (RFLP) (Metzler, Schudel, et al 1985;Rocha et al 1998;Takiuchi et al 2005;Kamiyoshi et al 2008) subtype-specific monoclonal antibodies (Metzler, Matile, et al 1985), restriction endonuclease mapping (Brake and Studdert 1985;Engels et al 1986), and characterization using single nucleotide polymorphism (SNPs) (Fulton et al 2016;Chase et al 2017) has been used to differentiate types and subtypes.…”

Section: Introductionmentioning

confidence: 99%

DNA Sequence variability analysis of the gD and the UL36 genes of Bovine herpesvirus-1 isolated from field cases in Indonesia

Noor

Untari

Haryadi

et al. 2019

Journal of Applied Animal Research

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To investigate the genotype of Bovine herpesvirus-1 (BHV-1) isolated in Indonesia (n = 10), the DNA sequences of fragments of two genes, including the middle third of the gD gene and the downstream end of UL36, were determined using nested PCR. All the samples were classified as BHV-1.2 according to the deduced amino acid sequence of gD. On the other hand, analysis of the nucleotide sequence of UL36 indicated the presence of insertions or deletions (indels) compared to reference sequences and each other, which indels made more diverse than in gD sequence, and the classification of BHV-1 subtype based on the deduced amino acid sequence of UL36 differed from that obtained using the gD protein sequence. These results suggested that while the gD sequence analysis was suited for rough classification of BHV-1 subtype, the UL36 sequence permitted detection of BHV-1 subtype polymorphisms.

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Section: Introductionmentioning

confidence: 99%

DNA Sequence variability analysis of the gD and the UL36 genes of Bovine herpesvirus-1 isolated from field cases in Indonesia

Noor

Untari

Haryadi

et al. 2019

Journal of Applied Animal Research

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“…and subtypes (1.1, 1.2), providing more opportunities for a balanced and targeted organization of health and preventive measures in the area of spread of infection(Schynts et al, 1999;Kamiyoshi et al, 2008;Yu et al, 2022).PCR provides special opportunities in the context of scientific research of RNA-containing viruses, in particular, such as the causative agents of Newcastle disease, highly pathogenic avian influenza, bovine viral diarrhea, border disease and classical swine fever, rabies and porcine respiratoryreproductive syndrome(Sullivan and Akkina, 1995;Alexander, 2008; Mari et al, 2015; Martínez-Bautista et al, 2018; Liang et al, 2019; Liu et al, 2022; Mao et al, 2022).…”

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confidence: 99%

Application of PCR and PCR-Based Techniques in Veterinary Medicine

Gerilovych,

Chechet,

Kovalenko

et al. 2023

OneHealthJournal

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New tests for the detection and typing of animal pathogens have been developed for veterinary medicine. Careful systematization is required to determine the place of molecular-based tools’ applications in the existing system of epizootological and epidemiological surveillance. Today, molecular genetic tests, including PCR, are used in veterinary medicine and agriculture for the following purposes:- surveillance and diagnosis of infectious and certain invasive diseases, - typing of animal pathogens, the study of their eco-geographic features, the drift of genetic variability and evolution, - research of molecular mechanisms of the immune response and the host-pathogen interactions, - quality and safety control of agricultural products, including food and feeds, - control of the quality and safety of genetic resources of animals, - control of the circulation of pathogens in the environment, - analysis of the origin and certification of breeds of productive and non-productive animals, etc. The application of molecular genetic methods of monitoring and early diagnosis is regulated by the Manual and Code of the World Organization for Animal Health (WOAH), the Program for the Global Control of Infectious Diseases of the World Health Organization, the guidelines on the monitoring of infectious diseases of animals and the control of the safety of agricultural products of the FAO. A large number of tests based on molecular diagnostic methods are recommended for use in infectious disease control programs, both emerging and economically significant, in the USA, Canada, and the countries of the European Union. This paper summarises the current PCR-based development scope and ways of its implementation in practical veterinary medicine.

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“…Table 3) [11] under the following conditions: 1 cycle at 96°C for 2 min, 35 or 40 cycles at 96°C for 20 s, 55°C for 30 s, and 72°C for 2 min; and 1 cycle at 72°C for 3 min. The results of real-time RT-PCR targeting BCoV showed that the Ct values of virus-spiked nasal swab samples were significantly lower in the LEAD DNA+RNA group than in the QIA group.…”

mentioning

confidence: 99%

“…Student’s t -test was used to identify the significant differences in Ct values for the LEAD DNA+RNA and QIA groups. Conventional PCR for IBRV was performed using DNA+RNA and DNA extracts as well as IBRV-specific primers ( Supplementary Table 3 ) [ 11 ] under the following conditions: 1 cycle at 96°C for 2 min, 35 or 40 cycles at 96°C for 20 sec, 55°C for 30 sec, and 72°C for 2 min; and 1 cycle at 72°C for 3 min. The results of real-time RT-PCR targeting BCoV showed that the Ct values of virus-spiked nasal swab samples were significantly lower in the LEAD DNA+RNA group than in the QIA group.…”

mentioning

confidence: 99%

Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017

Dong

Suzuki

Takemae

et al. 2022

The Journal of Veterinary Medical Science

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In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV) genome-positive cattle sera and bovine coronavirus/infectious bovine rhinotracheitis virus-spiked cattle nasal swabs. In addition, the subgenotyping of BVDV strains detected in Tokachi Province in Japan during 2016-2017 was performed. Results showed that most of these BVDV strains belonged to subgenotype 1b, while few strains belonged to subgenotypes 1a and 2a. This study showed the high applicability of automatic nucleic acid extractors in extracting multiple viral genomes and the dominant subgenotype of BVDV in Tokachi.

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The presence of a deletion sequence in the BHV-1 UL49 homolog in a live attenuated vaccine for infectious bovine rhinotracheitis (IBR)

Cited by 5 publications

References 45 publications

DNA Sequence variability analysis of the gD and the UL36 genes of Bovine herpesvirus-1 isolated from field cases in Indonesia

DNA Sequence variability analysis of the gD and the UL36 genes of Bovine herpesvirus-1 isolated from field cases in Indonesia

Application of PCR and PCR-Based Techniques in Veterinary Medicine

Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017

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