DNA Sequence variability analysis of the gD and the UL36 genes of Bovine herpesvirus-1 isolated from field cases in Indonesia

Noor, Hidayati Dewi; Untari, Tri; Haryadi, Wibowo Michael; Asmara, Widya; Akiyama, Koichi

doi:10.1080/09712119.2019.1600521

Cited by 2 publications

(3 citation statements)

References 26 publications

(27 reference statements)

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“…This study used a recent example from Lampung, and revealed a different result. Previous research by Noor et al (2019) resulted in a mixed allele using the AluI and TaqI restriction enzymes. The restriction endonuclease analysis (REA) methods could have instability because the genomic changes of the virus inside the host body, resulting from various tissue sources.…”

Section: Discussionmentioning

confidence: 99%

The establishment of PCR amplification, cloning, and sequencing of bovine herpesvirus 1 (BHV-1) glycoprotein D gene isolated in Indonesia

Hidayati

Srihanto

Untari

et al. 2019

Indones. J. Biotechnol.

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Considering the increasing incidence of infectious bovine rhinotracheitis (IBR) in Indonesia, it was necessary to conduct a more in-depth study of bovine herpesvirus-1 (BHV-1) as the causative agent of IBR disease. Previous research reports indicate that the BHV-1 subtypes found in Indonesia are subtype 1.1. Currently, IBR field case detection in Indonesia still uses the serological method (ELISA), which has the potential to give false positive results and cannot explain the virus subtype. Other detection methods, such as viral isolation, take longer and require adequate resources. This study aimed to determine the BHV-1 subtypes of Indonesian isolates using molecular techniques. Nested PCR using two pairs of primers was successfully used to amplify the glycoprotein D (gD) gene. The gD gene fragment was cloned into the pGEM-T plasmid. Analysis of the gD gene sequence was subsequently carried out to determine the BHV-1 character of the Indonesian isolates. The results indicated that the isolates were different from the previous isolates, and had similarities (100%) with subtype 1.2 strain SP1777 and SM023.

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Section: Discussionmentioning

confidence: 99%

The establishment of PCR amplification, cloning, and sequencing of bovine herpesvirus 1 (BHV-1) glycoprotein D gene isolated in Indonesia

Hidayati

Srihanto

Untari

et al. 2019

Indones. J. Biotechnol.

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“…The samples were characterized based on UL 36 genes and deposited on the DDBJ (DNA Data Bank of Japan) [14] as represented in Table 1. TABLE I. THE CHARACTERIZATION OF VIRAL SAMPLES BASED ON THE "GAP" OF UL36 GEN [14] III. RESULTS On the first day, the infected cells turn in to rounded and retractile cell, forming small gaps ( Fig.…”

Section: Viral Characterizationmentioning

confidence: 99%

“…The study of genetic variability in herpesvirus is interesting and always related to cellular phenotypic description [13]. According to the previous study, Indonesian IBR isolates collected from Lampung and Bukittinggi were belong to into subtype 1.1 and subtype 1.2 based on UL36 gene [14]. This research aim was to describe the phenotypic characterization of the Indonesian samples based on the insertion-deletion of UL36 gene characterization.that could subsequently initiate the entrance of bovine respiratory disease complex (BRDC) called "shipping fever".…”

Section: Introductionmentioning

confidence: 99%

Description of BHK-21Persistent Infected Cell Inoculated with Infectious Bovine Rhinotracheitis Virus

Hidayati¹,

Aini²,

Pancawidyana³

et al. 2019

Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia

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Infectious Bovine Rhinotracheitis (IBR) disease caused by Bovine Herpesvirus-1 (BHV-1), this diseases include in strategic diseases. Based on the genotype, this virus divided into several subtypes, including subtype 1.1; subtype 1.2a and subtype 1.2b. The previous genotypic study revealed that based on the insertion-deletion of UL36 gene, samples divided into subtype 1.1 and subtype 1.2. This study aim was comparing phenotypic images of isolates from Lampung and Bukittinggi in MDBK and BHK-21 cells. Samples of swab nasal and tracheal organ obtained from Balai Veteriner Lampung and Balai Veteriner Bukittinggi. The samples were cultured on MDBK and BHK-21 monolayer cell of 2 days old obtained from Pusvetma. The Cytopathogenic Effects (CPE) in MDBK and BHK-21 observed on day 2. In the MDBK cell, the CPE found as the grape-like cluster and apoptosis. The CPE of BHK-21 cell line showed syncytium and indicated persistent infection. At day four on BHK-21 cell, it observed the particular infected cell that showed differences in cell shape between samples that have proximity to subtype 1.1 and subtype 1.2. The infected cell of subtype 1.1 group, appear as elongated shape and linear pattern near the center area. Inversely, the samples grouped as subtype 1.2 appear as rounded cell and circular pattern of the center area.

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DNA Sequence variability analysis of the gD and the UL36 genes of Bovine herpesvirus-1 isolated from field cases in Indonesia

Cited by 2 publications

References 26 publications

The establishment of PCR amplification, cloning, and sequencing of bovine herpesvirus 1 (BHV-1) glycoprotein D gene isolated in Indonesia

The establishment of PCR amplification, cloning, and sequencing of bovine herpesvirus 1 (BHV-1) glycoprotein D gene isolated in Indonesia

Description of BHK-21Persistent Infected Cell Inoculated with Infectious Bovine Rhinotracheitis Virus

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