Description of BHK-21Persistent Infected Cell Inoculated with Infectious Bovine Rhinotracheitis Virus

Abstract

Infectious Bovine Rhinotracheitis (IBR) disease caused by Bovine Herpesvirus-1 (BHV-1), this diseases include in strategic diseases. Based on the genotype, this virus divided into several subtypes, including subtype 1.1; subtype 1.2a and subtype 1.2b. The previous genotypic study revealed that based on the insertion-deletion of UL36 gene, samples divided into subtype 1.1 and subtype 1.2. This study aim was comparing phenotypic images of isolates from Lampung and Bukittinggi in MDBK and BHK-21 cells. Samples of swab nasal and tracheal organ obtained from Balai Veteriner Lampung and Balai Veteriner Bukittinggi. The samples were cultured on MDBK and BHK-21 monolayer cell of 2 days old obtained from Pusvetma. The Cytopathogenic Effects (CPE) in MDBK and BHK-21 observed on day 2. In the MDBK cell, the CPE found as the grape-like cluster and apoptosis. The CPE of BHK-21 cell line showed syncytium and indicated persistent infection. At day four on BHK-21 cell, it observed the particular infected cell that showed differences in cell shape between samples that have proximity to subtype 1.1 and subtype 1.2. The infected cell of subtype 1.1 group, appear as elongated shape and linear pattern near the center area. Inversely, the samples grouped as subtype 1.2 appear as rounded cell and circular pattern of the center area.