The Preparation of Tissue-Type Plasminogen Activator (t-PA) Containing Liposomes: Entrapment Efficiency and Ultracentrifugation Damage

Heeremans, J.L.M.; Gerritsen, Hans; Meusen, S. P.; Mijnheer, F. W.; Panday, R. S. Gangaram; Prevost, R.; Kluft, C.; Crommelin, Daan J.A.

doi:10.3109/10611869509015959

Cited by 67 publications

(33 citation statements)

References 20 publications

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“…Formulation prepared with 2% soya lecithin and 30% ethanol (F5) showed 42% entrapment efficiency, which was increased to 61% when the concentration of ethanol increased to 35% (F6) respectively. These data supported by previous findings that solubility and high encapsulation efficiency values for large range of lipophilic drugs can be obtained due to presence of ethanol (13). From these results entrapment efficiency of formulation was observed due to increase in ethanol concentration.…”

Section: Entrapment Efficiencysupporting

confidence: 91%

Transdermal delivery of ethosomes as a novel vesicular carrier for paroxetine hydrochloride: In vitro evaluation and In vivo study

Pathan¹

2015

Marmara Pharm J

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The aim of the present work was to develop and characterisation of stable ethosomal formulation as a carrier for transdermal delivery of paroxetine hydrochloride. to prepare this ethosome, different concentrations of soya lecithin and ethanol were taken. Vesicular size, polydispersity index, zeta potential, entrapment effiency were determined by photon correlation spectroscopy and ultracentrifugation techniques. The in vitro permeation study across human cadaver skin was done. Stability study was done on optimised F2 formulation. The vesicle size decreases as an increase in the concentration of ethanol. entrapment efficiency increases with the increase in concentration of soya lecithin. The ethosome exhibit entrapment efficiency of 40-64%. In vitro permeation study across human skin ethosome F2 formulation showed higher transdermal flux 29.64% µg/cm 2 /hr. The release mechanism of in vitro permeation shows zero order drug release from the formulation. In vivo pharmacodyanamic study F2 formulation showed significant immobility as compared to control group. Stability study results revealed no significant change found in vesicle size distribution and polydispersity index. Our result indicates that the developed ethosomal system may be potential and safe for the delivery of paroxetine hydrochloride through skin.

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Section: Entrapment Efficiencysupporting

confidence: 91%

Transdermal delivery of ethosomes as a novel vesicular carrier for paroxetine hydrochloride: In vitro evaluation and In vivo study

Pathan¹

2015

Marmara Pharm J

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“…The free CF was separated from entrapped by using ultracentrifugation technique (Optima Max-E, Ultra Centrifuge, Beckman Coulter, Pasadena, CA) at 50,000 rpm at 4°C, for 30 min [36]. Liposome samples were removed when the PBS was clear.…”

Section: Methodsmentioning

confidence: 99%

Influence of the Flexible Liposomes on the Skin Deposition of a Hydrophilic Model Drug, Carboxyfluorescein: Dependency on Their Composition

Badran

Shalaby

Alomrani

2012

The Scientific World Journal

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This study focuses on the effect of different flexible liposomes containing sodium cholate, Tween 80, or cineol on skin deposition of carboxyfluorescein (CF). Size distribution, morphology, zeta potential, and stability of the prepared vesicles were evaluated. The influence of these systems on the skin deposition of CF utilizing rat skin as membrane model was investigated. Results showed that all of the investigated liposomes had almost spherical shapes with low polydispersity (PDI < 0.3) and particles size range from 83 to 175 nm. All liposomal formulations exhibited negative zeta potential, good drug entrapment efficiency, and stability. In vitro skin deposition data showed that flexible liposomes gave significant deposition of CF on the skin compared to conventional liposomes and drug solutions. This study revealed that flexible liposomes, containing cineole, were able to deliver higher amount of CF suggesting that the hydrophilic drugs delivery to the skin was strictly correlated to the vesicle composition.

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“…LMWH entrapment efficiency was determined on days 7 and 14 after separating the liposomes from the dispersion medium by centrifugation. 8,10,12,13 An aliquot of liposomes containing LMWH was mixed with 20 mL of water and then spun in an Optima TM LE-80K ultracentrifuge (Beckman Coulter, Inc., Fullerton, CA) using a 45Ti rotor at 158C and 40,000g for 30 min. LMWH content in the supernatant was determined using a colorimetric assay kit (Chromomeric Coatest Heparin Kit, Diapharma Group, Inc., West Chester, OH).…”

Section: Physicochemical Characteristics Of Liposomal Formulationsmentioning

confidence: 99%

Inhalable Liposomes of Low Molecular Weight Heparin for the Treatment of Venous Thromboembolism

Bai

Ahsan

2010

Journal of Pharmaceutical Sciences

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The Preparation of Tissue-Type Plasminogen Activator (t-PA) Containing Liposomes: Entrapment Efficiency and Ultracentrifugation Damage

Abstract: almost quantitative t-PA entrapment in liposomes can be achieved by selecting the proper milieu and inducing a strong interaction between t-PA and bilayer.

Cited by 67 publications

References 20 publications

Transdermal delivery of ethosomes as a novel vesicular carrier for paroxetine hydrochloride: In vitro evaluation and In vivo study

Transdermal delivery of ethosomes as a novel vesicular carrier for paroxetine hydrochloride: In vitro evaluation and In vivo study

Influence of the Flexible Liposomes on the Skin Deposition of a Hydrophilic Model Drug, Carboxyfluorescein: Dependency on Their Composition

Inhalable Liposomes of Low Molecular Weight Heparin for the Treatment of Venous Thromboembolism

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