The Preparation of Novel P(OEGMA-co-MEO2MA) Microgels-Based Thermosensitive Hydrogel and Its Application in Three-Dimensional Cell Scaffold

Abstract

Thermosensitive hydrogel scaffolds have attracted particular attention in three-dimensional (3D) cell culture. It is very necessary to develop a type of thermosensitive hydrogel material with low shrinkage, and excellent biocompatibility and biodegradability. Here, five types of thermosensitive microgels with different volume phase transition temperature (VPTT) or particle sizes were first synthesized using 2-methyl-2-propenoic acid-2-(2-methoxyethoxy) ethyl ester (MEO2MA) and oligoethylene glycol methyl ether methacrylate (OEGMA) as thermosensitive monomers by free radical polymerization. Their VPTT and particle sizes were investigated by a nanometer particle size meter and an ultraviolet spectrophotometer. The feasibility of using these P(OEGMA-co-MEO2MA) microgels to construct thermosensitive hydrogel by means of the thermal induction method is discussed for the first time. The prepared thermosensitive hydrogel with the optimum performance was screened for in situ embedding and three-dimensional (3D) culture of MCF-7 breast cancer cells. The experimental results of AO/EB and MTT methods indicate that the pioneering scaffold material has prominent biocompatibility, and cells grow rapidly in the 3D scaffold and maintain high proliferative capacity. At the same time, there is also a tendency to aggregate to form multicellular spheres. Therefore, this original P(OEGMA-co-MEO2MA) thermosensitive hydrogel can serve as a highly biocompatible and easily functionalized 3D cell culture platform with great potential in the biomedical area.

Modeling Tunable Fracture in Hydrogel Shell Structures for Biomedical Applications

“…The use of hydrogels as scaffolds has made further progress recently. For example, Liu et al [ 110 ] prepared a thermosensitive hydrogel and used it for in situ embedding and three-dimensional culture of MCF-7 breast cancer cells. It was shown that this thermosensitive hydrogel can be used as a three-dimensional platform with high biocompatibility and easy functionalization, and has great potential in the biomedical field.…”

Recent Advances in Hydrogel-Based Phototherapy for Tumor Treatment

“…As a result of this unique nanostructure, nanogels demonstrate a rapid response to changes in the environment. Accordingly, smart nanogels, also called stimuli-responsive nanogels, can be designed to achieve triggered drug release in response to various stimuli, such as light, ion-exchange, pH, and temperature [ 133 , 134 , 135 , 136 ]. The use of smart nanogels as a drug delivery system for anti-tumor phototherapy holds great promise, as evidenced by recent reports on thermosensitive nanogels [ 137 ], pH-sensitive nanogels [ 138 , 139 ], photosensitive nanogels [ 140 ], and redox-sensitive hydrogels [ 139 ].…”

Lokali̇ze Meme Kanseri̇ Tedavi̇leri̇nde Efekti̇f İlaç Taşiyici Si̇stemler: Enjektabl Hi̇drojeller

“…Farmasötik uygulamalarda genellikle sol-jel geçiş sıcaklığı için fizyolojik sıcaklık olan 37°C hedeflenmektedir [65]. Sıcaklık duyarlı hidrojel tasarımında kitozan, selüloz türevleri gibi doğal polimerler kullanılabilirken, sentetik kaynaklı olarak da poli N-izopropilakrilamid (pNiPAm), poli (etilen oksit)-b-poli (propilen oksit)-b-poli (etilen oksit) (PEO-PPO-PEO; Poloksamer), PEG-b-PCL, PEG-b-PLGA ve PEG-b-PLA gibi polimerler kullanılabilmektedir [66][67][68][69][70][71][72][73]. Lokal kanser uygulamalarında, in situ jel formülasyonlarında etkin maddenin çözelti formda çözünmüş olması, formülasyonun enjeksiyon sonrasında vücut sıcaklığındaki uygulama bölgesinde jel hale geçmesi, uzun süre stabilitesini koruyabilmesi ve enjektabl özelliğini koruması hedeflenmektedir [74].…”