The preparation of ciprofloxacin hydrochloride-loaded chitosan and pectin microspheres

Orhan, Zafer; Cevher, Erdal; Mülazımoğlu, Lütfiye; Gürcan, Duygu; Alper, Murat; Araman, Ahmet; Özsoy, Yıldız

doi:10.1302/0301-620x.88b2.16328

Cited by 35 publications

(19 citation statements)

References 51 publications

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“…Orhan et al (22) evaluated the efficacy of chitosan microspheres and pectin impregnated with ciprofloxacin in a model of local treatment of osteomyelitis and concluded that this type of treatment was higher than the equivalent treatment intramuscular antimicrobial. Stinner et al (16) evaluated the efficacy of a chitosan sponge impregnated with amikacin or vancomycin in a model of complex musculoskeletal wound and concluded that this treatment was effective in reducing the bacteria concentration within the wound.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Chitosan Films Impregnated With Ciprofloxacin for the Prophylaxis of Osteomyelitis in Open Fractures: An Experimental Study in Rats

et al. 2016

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BackgroundThe systemic antimicrobial prophylaxis is the standard treatment in the prevention of osteomyelitis after open fractures, with topical application of antimicrobials as an alternative due to their high concentrations at the site of the fracture, low systemic concentrations and fewer side effects.ObjectivesThis study aimed to evaluate the effectiveness of prophylaxis of osteomyelitis through experimental model of open fractures with the use of chitosan films, whether or not impregnated with ciprofloxacin.Materials and MethodsIn this experimental study, 24 Holtzman rats were distributed into 4 groups of 6 rats each. The CT (control of treatment) group: an open fracture model treated with systemic antimicrobial; the IC (infection control) group: an open fracture untreated model; the C (chitosan) group: an open fracture model treated using a chitosan film; and the CA (chitosan with antimicrobial) group: an open fracture model treated using a chitosan film impregnated with antimicrobial. After 3 weeks the animals were killed by an overdose of anesthetic, and a fragment osseous was removed for histological and microbiological analysis. The comparisons between the groups considered significant values of P ≤ 0.05.ResultsIn cultures of the CT group, there was less bacterial growth compared to the results of the cultures of the IC (P = 0.005), C (P = 0.005) and CA (P = 0.009) groups. The inflammation was lower in the CT group compared to the IC (P = 0.014), C (P = 0.001) and CA (P = 0.007) groups.ConclusionsIn this experimental model of open fracture, the chitosan film pure or impregnated with ciprofloxacin was not effective in the prophylaxis of osteomyelitis.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of Chitosan Films Impregnated With Ciprofloxacin for the Prophylaxis of Osteomyelitis in Open Fractures: An Experimental Study in Rats

et al. 2016

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“…[13][14][15][16] Among other local delivery systems are antibiotic-loaded collagen gauze bands, antibioticloaded bone structured materials and polyurethane implants that can be applied over external fixation pins to avoid pin-track infection. [17][18][19][20] All these methods need a carrier other than the implant itself to release the antibiotic. They may also require a further operation for its removal, as with bone cement spacers.…”

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confidence: 99%

Evaluation and comparison of the antimicrobial efficacy of teicoplanin- and clindamycin-coated titanium implants

Aykut

Öztürk

Özkan

et al. 2010

The Journal of Bone and Joint Surgery. British volume

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We studied the effects of coating titanium implants with teicoplanin and clindamycin in 30 New Zealand White rabbits which were randomly assigned to three groups. The intramedullary canal of the left tibia of each rabbit was inoculated with 500 colony forming units of Staphylococcus aureus. Teicoplanin-coated implants were implanted into rabbits in group 1, clindamycin-coated implants into rabbits in group 2, and uncoated implants into those in group 3. All the rabbits were killed one week later. The implants were removed and cultured together with pieces of tibial bone and wound swabs. The rate of colonisation of the organisms in the three groups was compared. Organisms were cultured from no rabbits in group 1, one in group 2 but from all in group 3. There was no significant difference between groups 1 and 2 (p = 1.000). There were significant differences between groups 1 and 3 and groups 2 and 3 (p < 0.001). Significant protection against bacterial colonisation and infection was found with teicoplanin- and clindamycin-coated implants in this experimental model.

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“…Antibiotics may be given locally by various methods such as implantable antibiotic pumps, 50 polymethylmethacrylate beads, 51 hydroxyapatite cement, 52 biodegradable microspherical implants (microspheres prepared with chitosan, 53 polylactide glycolide microspherical implants 54 ). Osteomyelitis treatment requires a long period of antibiotic therapy with high serum concentrations.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of moxifloxacin compared to teicoplanin in the treatment of implant‐related chronic osteomyelitis in rats

Özturan

Yücel

Koçoğlu

et al. 2010

Journal Orthopaedic Research

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Treatment of implant-related chronic osteomyelitis is often difficult and usually consists of implant removal, extensive surgical debridement, and prolonged antibiotic use. This study was performed to assess the efficacy of moxifloxacin compared to a glycopeptide, teicoplanin in chronic implant-related methicillin-sensitive Staphylococcus aureus (MSSA) osteomyelitis. The left femoral medullar cavities of 60 Wistar male rats were contaminated with 100 l of 10 8 cfu/ml methicillin-sensitive S. aureus (ATCC 29213) and Kirschner wires were placed into the medulla of the femur. After 6 weeks, rats were randomly divided into five groups. In two groups, the Kirschner wires were removed. Experimental groups were as follows: group 1: contaminated, Kirschner wire inside, received teicoplanin; group 2: contaminated, Kirschner wire removed, received teicoplanin; group 3: contaminated, Kirschner wire inside, received moxifloxacin; group 4: contaminated, Kirschner wire removed, received moxifloxacin; group 5: contaminated, Kirschner wire inside, no antibiotics (control group). Groups 1 and 2 received teicoplanin (20 mg/kg once daily), whereas groups 3 and 4 received moxifloxacin (10 mg/kg twice daily) intraperitoneally for 28 days. At the end of the treatment, animals were sacrificed by inhalation anesthesia with ether and femora were retrieved and bacterial counts (cfu/g) were determined. Bacterial counts in all study groups were significantly reduced relative to the control. The decrease of bacterial counts was more prominent in group 4 compared to group 1 (p = 0.001) and group 2 (p = 0.003). Moxifloxacin therapy is an effective alternative to teicoplanin for chronic implant-related MSSA osteomyelitis.

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The preparation of ciprofloxacin hydrochloride-loaded chitosan and pectin microspheres

Cited by 35 publications

References 51 publications

Effectiveness of Chitosan Films Impregnated With Ciprofloxacin for the Prophylaxis of Osteomyelitis in Open Fractures: An Experimental Study in Rats

Effectiveness of Chitosan Films Impregnated With Ciprofloxacin for the Prophylaxis of Osteomyelitis in Open Fractures: An Experimental Study in Rats

Evaluation and comparison of the antimicrobial efficacy of teicoplanin- and clindamycin-coated titanium implants

Efficacy of moxifloxacin compared to teicoplanin in the treatment of implant‐related chronic osteomyelitis in rats

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