The preparation and evaluation of superior bonded phases for reversed-phase, high-performance liquid chromatography

Evans, M. Blair; Dale, A.D.; Little, C.J.

doi:10.1007/bf02302709

Cited by 67 publications

(18 citation statements)

References 15 publications

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“…RPl8 reverse-phase TLC plates were prepared from the Merck plates. 26 Medium-pressure column chromatography 27 was carried out using Merck silica gel 60 (Product No. 151 1 1) which was packed in the starting solvent.…”

Section: Methodsmentioning

confidence: 99%

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Synthesis of enterochelin

Rogers¹

1995

J. Chem. Soc., Perkin Trans. 1

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Section: Methodsmentioning

confidence: 99%

“…151 11 . 26 Compounds were detected if possible, by examining the TLC plates under UV light. Amines were detected by spraying with 0.16% ninhydrin: 0.16% s-collidine in butanone followed by heating.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of enterochelin

Rogers¹

1995

J. Chem. Soc., Perkin Trans. 1

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“…There are two approaches to alleviating the influence of charge. Using one that is common in liquid chromatography, end-capping the silica with methyl groups [65] eliminated the influence of pH and ionic strength [64]. The second method is to use as the sol a trialkylalkoxysilane so that the product is an ormosil without charged groups [32].…”

Section: Influence Of Surface Charge On Nanoporous Silanebased Filmsmentioning

confidence: 99%

“…A similar electrode was used for the electrocatalytic reduction of bromate and oxidation of methionine [73]. Here, the silica was endcapped [65] to eliminate its anionic sites, and gold nanoparticles modified with dirhodium phosphomolybdate (AuNP-Rh 2 PMo) [73] were bound to the pore-base electrode interface. The flow-injection analysis of bromate under potentiostatic conditions yielded a linear dynamic range of 0.2-10 μmol dm −3 and a detection limit (k = 3 criterion) of 30 nmol dm −3 .…”

Section: Applications Of Sol-gel Modified Electrodesmentioning

confidence: 99%

Electroanalysis based on stand-alone matrices and electrode-modifying films with silica sol-gel frameworks: a review

Miecznikowski

Cox

2020

J Solid State Electrochem

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Silica sol-gel matrices and its organically modified analogues that contain aqueous electrolytes, ionic liquids, or other ionic conductors constitute stand-alone solid-state electrochemical cells when hosting electrodes or serve as modifying films on working electrodes in conventional cells. These materials facilitate a wide variety of analytical applications and are employed in various designs of power sources. In this review, analytical applications are the focus. Solid-state cells that serve as gas sensors, including in chromatographic detectors of gas-phase analytes, are described. Sol-gel films that modify working electrodes to perform functions such as hosting electrochemical catalysts and acting as size-exclusion moieties that protect the electrode from passivation by adsorption of macromolecules are discussed with emphasis on pore size, structure, and orientation. Silica sol-gel chemistry has been studied extensively; thus, factors that control its general properties as frameworks for solid-state cells and for thin films on the working electrode are well characterized. Here, recent advances such as the use of dendrimers and of nanoscale beads in conjunction with electrochemically assisted deposition of silica to template pore size and distribution are emphasized. Related topics include replacing aqueous solutions as the internal electrolyte with room-temperature ionic liquids, using the sol-gel as an anchor for functional groups and modifying electrodes with silica-based composites.

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“…4-Azido-2-hydroxybenzoic acid hydroxysuccinimide ester was purchased from Pierce. Reversed-phase silica (RP-8) was prepared as described by Evans et al (1980). 45Ca and I4C molecular weight markers were obtained from Amersham International.…”

Section: General Materialsmentioning

confidence: 99%

Construction of a Physiologically Active Photoaffinity Probe Based on the Structure of Bradykinin: Labelling of Angiotensin Converting Enzyme but Not Candidate Bradykinin Receptors on NG108–15 Cells

Vries¹,

Phillips²,

Snell³

et al. 1989

Journal of Neurochemistry

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The peptides bradykinin and kallidin are released in response to noxious stimuli and mediate various physiological effects, including a direct stimulation of nociceptive afferent neurones. The nature of the receptor molecules through which these ligands act is presently unknown. We synthesised an iodinatable photoaffinity probe, N epsilon-4-azidosalicylylkallidin, and used it in an attempt to identify candidate bradykinin receptors on the NG108-15 neuroblastoma X glioma hybrid cell line. The ligand bound in subdued light to a particulate fraction of NG108-15 tumours and could be displaced by bradykinin with an IC50 of 0.33 nM. In a physiological assay, it behaved as an agonist equipotent with bradykinin. Gel analysis of the labelled products after photolysis of the iodinated ligand in the presence of NG108-15 cells or tumour membranes revealed bradykinin-blockable labelling of a glycoprotein with an Mr of 166,000. The probe was also able to label purified commercial angiotensin converting enzyme. The band labelled in NG108-15 cells was immunoprecipitable with a polyclonal antiserum to angiotensin converting enzyme, an enzyme shown to be present in low amounts in these preparations by direct binding using the iodinatable specific ligand MK351A.

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The preparation and evaluation of superior bonded phases for reversed-phase, high-performance liquid chromatography

Cited by 67 publications

References 15 publications

Synthesis of enterochelin

Synthesis of enterochelin

Electroanalysis based on stand-alone matrices and electrode-modifying films with silica sol-gel frameworks: a review

Construction of a Physiologically Active Photoaffinity Probe Based on the Structure of Bradykinin: Labelling of Angiotensin Converting Enzyme but Not Candidate Bradykinin Receptors on NG108–15 Cells

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