The Preparation and Evaluation of a Standardized Fibrin Plate for the Assessment of Fibrinolytic Activity

Abstract

SummaryA new fibrin plate technic for evaluating components of the fibrinolytic system has been developed. It provides quick, accurate, and easily interpreted results for the fibrinolytic profile. The standardized human plasminogen-free fibrin plates can be produced in bulk and stored for prolonged periods of time. A test specimen placed in a well punched in the buffered agarose gel diffuses into the agar and lyses the fibrin clot, forming a clear reaction zone. The zone diameter is directly proportional to the log of the percent concentration of available fibrinolytic enzyme in the specimen. The plates may be used to quantitate total plasminogen, and estimate available plasmin and active plasmin. A good correlation between results obtained using these fibrin plates and caseinolytic methods was found. Performance and interpretation of tests of fibrinolysis done on these new fibrin plates indicate that it may be the most sensitive technic available for clinical laboratory work.

Epsilon-Aminocaproic Acid for Synovectomy in Haemophilic Patients

“…To test the global fibrinolytic activity, the follow ing methods were used: the caseinolysis method of Bruhn et al [5]; the fibrin plate method according to A strup and M ullertz [3]; the plasminogen-free fibrin plate method according to Bishop et al [4]. The same group of methods was also used when employing both simple and streptokinase and urokinase-activated euglobulin fractions.…”

Fibrinolysis during Extracorporeal Circulation

“…Plasmin and antiplasmin were estimated on plasminogen-free human fibrin-agar plates. The preparation and standardization of these has previously been described (6). Plasmin was estimated by placing 10 ^.liters of the patient's plasma in the wells of the fibrin-agar plates and measuring the diameter of the lysis zone after 24 hours of incubation at 37°C.…”

Large scale, rapid purification of recombinant tissue‐type plasminogen activator

“…TAG system [17]. Fibrinolytic activity was measured essentially as before [18]; calibration was against the international standard for t-PA, Lot 83/517.…”