Recombinant tissue-type plasminogen activator (r&PA) from cultures of a genetically manipulated Bowes melanoma cell line (TRBM6) was purified in batches of average volume 451 using an autoclavable, reusable, continuous chromatography system comprising zinc chelate-Sepharose CL4B and lysine-Sepharose CL4B. After eight successive purifications the r&PA was ultrafiltered to yield a preparation containing 4.9 mg protein/ml and 2.7 x lo6 IU/ml. Analysis by SDS-polyacrylamide gel electrophoresis followed by staining with Coomassie brilliant blue R250 showed major protein bands at M, = 63000 and 65000, most of the material was in the l-chain form. The potential usefulness of a simple, rapid continuous chromatography system that can be operated under aseptic conditions is discussed.Recombinant t-PA Zinc chelate-Sepharose CUB Lysine-Sepharose CUB Continuous chromatography
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