2013
DOI: 10.1371/journal.pgen.1003297
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The Prefoldin Bud27 Mediates the Assembly of the Eukaryotic RNA Polymerases in an Rpb5-Dependent Manner

Abstract: The unconventional prefoldin URI/RMP, in humans, and its orthologue in yeast, Bud27, have been proposed to participate in the biogenesis of the RNA polymerases. However, this role of Bud27 has not been confirmed and is poorly elucidated. Our data help clarify the mechanisms governing biogenesis of the three eukaryotic RNA pols. We show evidence that Bud27 is the first example of a protein that participates in the biogenesis of the three eukaryotic RNA polymerases and the first example of a protein modulating t… Show more

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Cited by 71 publications
(122 citation statements)
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References 56 publications
(116 reference statements)
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“…Recent observations in yeast and humans are consistent with RNA pol assembly in the cytoplasm as a prerequisite for its nuclear import (23,(30)(31)(32). Thus, our results do not eliminate the possibility that RNA pol II foot mutations affect the correct assembly of Rpb1 with the rest of the complex and that, thus, nonassociated Rpb1 could be degraded in the cytoplasm.…”
Section: Resultssupporting
confidence: 45%
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“…Recent observations in yeast and humans are consistent with RNA pol assembly in the cytoplasm as a prerequisite for its nuclear import (23,(30)(31)(32). Thus, our results do not eliminate the possibility that RNA pol II foot mutations affect the correct assembly of Rpb1 with the rest of the complex and that, thus, nonassociated Rpb1 could be degraded in the cytoplasm.…”
Section: Resultssupporting
confidence: 45%
“…Thus, our results do not eliminate the possibility that RNA pol II foot mutations affect the correct assembly of Rpb1 with the rest of the complex and that, thus, nonassociated Rpb1 could be degraded in the cytoplasm. To help elucidate this possibility, we tested the localization and accumulation of Rpb1 upon deletion of BUD27, a prefoldin whose inactivation alters the assembly of RNA pol II, leading to its cytoplasmic accumulation (23). In immunocytochemistry experiments, clear Rpb1 cytoplasmic accumulation was found in a bud27⌬ mutant strain, although nuclear staining was also detected, indicating that RNA pol II was partially mislocalized (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In S. cerevisiae, assembly of the RNA pols occurs in the cytoplasm prior their entry to the nucleus, and Rpb6 and Rpb5 assemble in a process dependent on the prefoldin-like Bud27 [42]. Similarly, cytoplasmic RNA pol I assembly has been previously proposed in human [44].…”
Section: The Yeast Role In Medical Applications 154mentioning
confidence: 63%
“…In accordance with the role of Rpb6 in RNA pols assembly, the lack of Bud27 alters the correct cytoplasmic assembly of Rpb5 and Rpb6 to the three RNA polymerases, leading to a more instable RNA pol II [42]. Intriguingly, of the five shared subunits, both Rpb6 and Rpb5 have two paralogues in Trypanosome brucei, T. cruzi and Leishmania major [17].…”
Section: The Yeast Role In Medical Applications 154mentioning
confidence: 86%
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