Subunits Common to RNA Polymerases

Cuevas-Bermúdez, Abel; Martínez-Fernández, Verónica; Garrido-Godino, Ana I.; Navarro, Francisco

doi:10.5772/intechopen.70936

Cited by 4 publications

(3 citation statements)

References 82 publications

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“…These small subunits have no known equivalent in the eubacterial enzyme. They are conserved in a single RNA polymerase from Archaea, but a homologue of Rpb8 has been identified only in some archaeal species ( 2 , 3 ). Common small subunits either bind or bridge catalytic subunits that divide the polymerase core into interacting subassemblies ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

The expression of Rpb10, a small subunit common to RNA polymerases, is modulated by the R3H domain-containing Rbs1 protein and the Upf1 helicase

Cieśla

Turowski

Nowotny

et al. 2020

Nucleic Acids Research

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The biogenesis of eukaryotic RNA polymerases is poorly understood. The present study used a combination of genetic and molecular approaches to explore the assembly of RNA polymerase III (Pol III) in yeast. We identified a regulatory link between Rbs1, a Pol III assembly factor, and Rpb10, a small subunit that is common to three RNA polymerases. Overexpression of Rbs1 increased the abundance of both RPB10 mRNA and the Rpb10 protein, which correlated with suppression of Pol III assembly defects. Rbs1 is a poly(A)mRNA-binding protein and mutational analysis identified R3H domain to be required for mRNA interactions and genetic enhancement of Pol III biogenesis. Rbs1 also binds to Upf1 protein, a key component in nonsense-mediated mRNA decay (NMD) and levels of RPB10 mRNA were increased in a upf1Δ strain. Genome-wide RNA binding by Rbs1 was characterized by UV cross-linking based approach. We demonstrated that Rbs1 directly binds to the 3′ untranslated regions (3′UTRs) of many mRNAs including transcripts encoding Pol III subunits, Rpb10 and Rpc19. We propose that Rbs1 functions by opposing mRNA degradation, at least in part mediated by NMD pathway. Orthologues of Rbs1 protein are present in other eukaryotes, including humans, suggesting that this is a conserved regulatory mechanism.

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Section: Introductionmentioning

confidence: 99%

The expression of Rpb10, a small subunit common to RNA polymerases, is modulated by the R3H domain-containing Rbs1 protein and the Upf1 helicase

Cieśla

Turowski

Nowotny

et al. 2020

Nucleic Acids Research

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show abstract

“…Transcription in bacteria and archaea is carried out by a single multimeric RNA polymerase, while most eukaryotes contains three multimeric complexes (RNA pol I, II, and III) [1][2][3]. Furthermore, plants contain two additional RNA pols (IV and V) that have evolved from RNA pol II [4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

RNA polymerases IV and V are involved in olive fruit development

Serrano,

Moret,

Fernández-Parras

et al. 2023

Preprint

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Transcription is carried out in most eukaryotes by three multimeric complexes (RNA polymerases I, II and III). However, plants contain two additional RNA polymerases (IV and V), which have evolved from RNA polymerase II. RNA polymerases II, IV and V contain both common and specific subunits that may specialize some of their functions. In this study, we conducted a search for the genes that putatively code for the specific subunits of RNA polymerases IV and V, as well as the corresponding of RNA polymerase II in olive. Based on the homology with the genes of Arabidopsis thaliana, we identified 13 genes that putatively code for the specific subunits of polymerases IV and V, and 16 genes that code for the corresponding specific subunits of polymerase II in olive. The transcriptomic analysis by RNA-Seq revealed that the expression of the RNA polymerases IV and V genes was induced during the initial stages of fruit development. Given that RNA polymerases IV and V are involved in the transcription of long non-coding RNAs, we investigated their expression and observed relevant changes in the expression of this type of RNAs. Particularly, the expression of the intergenic and intronic long non-coding RNAs tended to increase in the early steps of fruit development, suggesting their potential role in this process. The positive correlation between the expression of RNA polymerases IV and V subunits and the expression of non-coding RNAs supports the hypothesis that RNA polymerases IV and V may play a role in fruit development throughout the synthesis of this type of RNAs.

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“…Transcription in bacteria and archaea is carried out by a single multimeric RNA polymerase, while most eukaryotes contain three multimeric complexes (RNA pol I, II, and III) [1][2][3]. Furthermore, plants contain two additional RNA pols (IV and V) that have evolved from RNA pol II [4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

RNA Polymerases IV and V Are Involved in Olive Fruit Development

Serrano,

Moret,

Fernández-Parras

et al. 2023

Preprint

View full text Add to dashboard Cite

Transcription is performed in most eukaryotes by three multimeric complexes (RNA polymer-ases I, II and III). However, plants contain two additional RNA polymerases (IV and V), which have evolved from RNA polymerase II. RNA polymerases II, IV and V contain both common and specific subunits that may specialize some of their functions. We herein searched for the genes that putatively code for the specific subunits of RNA polymerases IV and V, and the cor-responding of RNA polymerase II in olive. Based on the homology with the genes of Arabidop-sis thaliana, 13 genes that putatively code for the specific subunits of polymerases IV and V, and 16 genes that code for the corresponding specific subunits of polymerase II, were identified in olive. The expression analysis in different organs/tissues, biotic and abiotic stresses and a de-velopment process revealed that the expression of the RNA polymerases IV and V genes was induced during the initial fruit development steps. In lines with RNA polymerases IV and V in-volved in the transcription of long non-coding RNAs this induction was accompanied by rele-vant changes in expression of this type of RNAs. The expression of the intergenic and intronic long non-coding RNAs tended to increase in the early steps of developing fruit. These changes in the expression of long non-coding RNAs may be important for controlling gene expression during fruit development. In addition, some intergenic transcripts are susceptible to be pro-cessed and to become siRNAs, which are known to play a role in gene expression control. This reinforces the hypothesis that RNA polymerases IV and V may play a role in fruit development throughout the synthesis of long non-coding RNAs.

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Subunits Common to RNA Polymerases

Cited by 4 publications

References 82 publications

The expression of Rpb10, a small subunit common to RNA polymerases, is modulated by the R3H domain-containing Rbs1 protein and the Upf1 helicase

The expression of Rpb10, a small subunit common to RNA polymerases, is modulated by the R3H domain-containing Rbs1 protein and the Upf1 helicase

RNA polymerases IV and V are involved in olive fruit development

RNA Polymerases IV and V Are Involved in Olive Fruit Development

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