The predictability of subnormal penetrating capacity of sperm in cases of unexplained infertility

Aitken, R. John; Warner, Pamela; Best, Fiona S.M.; Templeton, Allan; Djahanbakhch, O.; Mortimer, David; Lees, M. M.

doi:10.1111/j.1365-2605.1983.tb00534.x

Cited by 28 publications

(14 citation statements)

References 17 publications

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“…Utilizing two in vitro tests of sperm function, we assessed the hypothesis that the first portion of an ejaculated semen specimen has better fertilizing capacity than either the second portion or the whole semen sample. The two tests used, the zona-free hamster ova in vitro penetration test and the cervical mucus penetration test have been demonstrated to correlate with fertilization of human eggs in vitro (Wolf et al -1983) and with pregnancy (Margoliothe et al -1983;Alexander -1981and Aitken et al -1983. In our study, no significant differences were found among penetration rates of sperm collected in fraction I, fraction 11, or the whole ejaculate using either test system.…”

Section: Discussioncontrasting

confidence: 44%

The Split Ejaculate: Assessment of Fertility Potential Using Two in vitro Test Systems

et al. 2009

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Zusammenfassung: Von 12 normalen Miinnern (Gruppe A) und 8 infertilen Miinnern mit Oligozoospennie bei Spermatozoenkonzentrationen von weniger als 20 x lo6 /ml (Gruppe B) wurden Samenproben (Split-und vollstiindige Ejakulate) untersucht. M e Samenproben wurden untersucht mit StandardSamen Analyse, Rinderzervikalschleim-Penetrationsassay (CMPT) und in allen Fiillen mit hinreichendem Samen im Hmanspermatozoen-zonafreiesHamsterei-Penetrationsassay (SPA).

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Section: Discussioncontrasting

confidence: 44%

The Split Ejaculate: Assessment of Fertility Potential Using Two in vitro Test Systems

et al. 2009

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“…Exposing the spermatozoa to ROS, causes DNA damage and lipid peroxidation (36). High lipid peroxidation may lead to reduce acrosomal reaction, fertilization (37) , and sperm oocyte fusion.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Sperm Parameters With Semen Lipid Peroxidation and Total Antioxidants Levels in Astheno- and Oligoasheno- Teratospermic Men

Colagar¹,

Karimi²,

Jorsaraei³

2013

Iran Red Crescent Med J

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Background: Sperm dysfunction caused by reactive oxygen species (ROSs) is one of the major causes of infertility in men, which leads to, lipid peroxidation (LPO) and the formation of stable peroxidation products like Malondialdehyde (MDA) in seminal plasma. MDA is effective factor in reducing fertility. Objectives: The aim of this study is to determine two biochemical markers of oxidative stress; TAC and MDA, and them correlation to quality-quantity factors in Asthenoteratospermic and Oligoashenoteratospermic men. Patients and Methods: A total of 42 semen samples including: 15 samples normospermic as control group, 12 Asthenoteratospermic and 15 oligoasthenoteratospermic were collected from Babol IVF center; Iran. Semen analysis was performed according to WHO (1999) guidelines. Seminal plasma TAC and MDA levels in all patients were measured by TBARs and FRAP methods, respectively. Results: Seminal plasma TAC level in normospermic men was significantly higher than asthenoteratospermic men (P < 0.001) and oligasthenoteratospermic men (P < 0.001) and had posetive correlation with sperm count, motility and morphology. In contrast MDA levels in normospermic men were significantly lower than in asthenoteratospermic men (P = 0.049) and oligoasthenoteratospermic men (P = 0.001) and had negative correlation with sperm count, motility and morphology. Conclusions: These results suggest that lipid peroxidation and decreasing total antioxidant capacity lead to low motility; morphology and sperm count in spermatozoa of astheno-and oligoastheno-teratospermic men. Therefore, evaluation of oxidative status and antioxidant defenses system may be as a useful tool for diagnosis and treatment of male infertility especially in idiopathic male infertility. Keywords: Lipid Peroxidation; Malondialdehyde; Reactive Oxygen SpeciesImplication for health policy/practice/research/medical education: Lipid peroxidation and decreasing total antioxidant capacity lead to low motility, morphology and sperm count in spermatozoa of astheno -and oligoastheno -teratospermic men.

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“…Motility stimulants elevate sperm MOT, VCL, and ALH, and consistently increase the fertilization rates of cryopreserved or teratospermic human spermatozoa [2,10,12,271. We have used computer-assisted semen analysis (CASA) to examine the ability of the motility stimulants, caffeine, pentoxifylline, and 2'-deoxyadenosine, to increase the motility of epididymal cat sperm compromised by cryopreservation [2 11.…”

mentioning

confidence: 99%

Stimulation of Ejaculated Domestic Cat Sperm Motility with Caffeine, Pentoxifylline, and 2′-Deoxyadenosine

Stachecki

Dresser

Pope

et al. 1995

Archives of Andrology

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: We have investigated the effects of caffeine, pentoxifylline, and 2′‐deoxyadenosine on the motion characteristics and longevity of domestic cat spermatozoa. Freshly collected or cryopreserved domestic cat epididymal sperm were incubated with 0.01–20 mM caffeine, pentoxifylline, or 2′‐deoxyadenosine for 15 minutes at 23°C. The percent motility (MOT), curvilinear velocity (VCL), linearity (LIN), straight line velocity (VSL), and amplitude of lateral head displacement (ALH) were determined for each group using computer‐assisted semen analysis. Freshly collected domestic cat sperm exhibited a strong forward progressive movement, and treatment with caffeine, pentoxifylline, or 2′‐deoxyadenosine did not consistently alter sperm motion. Following cryopreservation, spermatozoa exhibited decreased (P < 0.05) MOT, VCL, VSL, and ALH. Caffeine and pentoxifylline increased (P < 0.05) the MOT, VSL, VCL, and ALH of cryopreserved sperm at 0.01–20 mM, in a dose‐dependent manner. 2′‐Deoxyadenosine also increased (P < 0.05) both VSL and VCL at 1.0 mM, and MOT, VSL, VCL, and ALH at 10 mM. All treatments shifted the percentage of nonhyperactive sperm to either a transitional or hyperactivated state. The motility indices of cryopreserved samples were examined during a 6‐ hour incubation to assess the effects of caffeine, pentoxifylline, and 2′‐deoxyadenosine on sperm longevity. Compared to untreated control samples, the longevity of stimulated cryopreserved sperm was not reduced. These results indicate that motility stimulants may prove useful for enhancing the fertility of cryopreserved cat sperm by increasing their motility and producing hyperactivated motion.

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The predictability of subnormal penetrating capacity of sperm in cases of unexplained infertility

Cited by 28 publications

References 17 publications

The Split Ejaculate: Assessment of Fertility Potential Using Two in vitro Test Systems

The Split Ejaculate: Assessment of Fertility Potential Using Two in vitro Test Systems

Correlation of Sperm Parameters With Semen Lipid Peroxidation and Total Antioxidants Levels in Astheno- and Oligoasheno- Teratospermic Men

Stimulation of Ejaculated Domestic Cat Sperm Motility with Caffeine, Pentoxifylline, and 2′-Deoxyadenosine

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