Correlation of Sperm Parameters With Semen Lipid Peroxidation and Total Antioxidants Levels in Astheno- and Oligoasheno- Teratospermic Men

Abstract

Background: Sperm dysfunction caused by reactive oxygen species (ROSs) is one of the major causes of infertility in men, which leads to, lipid peroxidation (LPO) and the formation of stable peroxidation products like Malondialdehyde (MDA) in seminal plasma. MDA is effective factor in reducing fertility. Objectives: The aim of this study is to determine two biochemical markers of oxidative stress; TAC and MDA, and them correlation to quality-quantity factors in Asthenoteratospermic and Oligoashenoteratospermic men. Patients and Methods: A total of 42 semen samples including: 15 samples normospermic as control group, 12 Asthenoteratospermic and 15 oligoasthenoteratospermic were collected from Babol IVF center; Iran. Semen analysis was performed according to WHO (1999) guidelines. Seminal plasma TAC and MDA levels in all patients were measured by TBARs and FRAP methods, respectively. Results: Seminal plasma TAC level in normospermic men was significantly higher than asthenoteratospermic men (P < 0.001) and oligasthenoteratospermic men (P < 0.001) and had posetive correlation with sperm count, motility and morphology. In contrast MDA levels in normospermic men were significantly lower than in asthenoteratospermic men (P = 0.049) and oligoasthenoteratospermic men (P = 0.001) and had negative correlation with sperm count, motility and morphology. Conclusions: These results suggest that lipid peroxidation and decreasing total antioxidant capacity lead to low motility; morphology and sperm count in spermatozoa of astheno-and oligoastheno-teratospermic men. Therefore, evaluation of oxidative status and antioxidant defenses system may be as a useful tool for diagnosis and treatment of male infertility especially in idiopathic male infertility. Keywords: Lipid Peroxidation; Malondialdehyde; Reactive Oxygen SpeciesImplication for health policy/practice/research/medical education: Lipid peroxidation and decreasing total antioxidant capacity lead to low motility, morphology and sperm count in spermatozoa of astheno -and oligoastheno -teratospermic men.

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

“…In addition, they documented higher MDA levels in the seminal plasma of asthenoteratozoospermic and oligoasthenoteratozoospermic men and MDA's negative correlation with sperm count, motility, and normal morphology (21). In another study, Patel et al observed elevated levels of MDA in all infertile groups, except the azoospermic group, compared to fertile men.…”

“…Because spermatozoa possess high amounts of polyunsaturated fatty acids in their plasma membranes, they are predisposed to oxidative injury. Therefore, they are susceptible to radical attack and consequently to lipid peroxidation in the plasma membrane (8,21). ROS could also induce base alterations, DNA strand breaks, DNA cross-links, and chromosomal rearrangements (22).…”

Association of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Infertile Men With Varicocele

“…Excessive amounts of ROS may lead to lipid peroxidation, loss of motility, and sperm DNA damage (20,21). There are high amounts of polyunsaturated fatty acids in the plasma membranes of spermatozoa, therefore, they are predisposed to oxidative damage (5,22). In addition, ROS could induce base alterations, DNA strand breaks, DNA cross-links, and chromosomal rearrangements (23).…”

Oxidative stress level in fresh ejaculate is not related to semen parameters or to pregnancy rates in cycles with donor oocytes

“…OS has been found to promote a dose-dependent increase of tyrosine nitration and S-glutathionylation in the sperm, and this change relates to an alteration in motility and the ability of spermatozoa to undergo capacitation [24]. Lipid peroxidation (an indicator of oxidative status) and decreased antioxidant capacity in the ejaculate lead to low motility in asthenoteratozoospermic and oligoasthenoteratozoospermic men; morphology and sperm count were also lower [25]. We found no significant differences in sperm motility between asthenozoospermic and normozoospermic patients among the cases with high and very high OS (52 vs 41.7 %; Table 1), in agreement with a recent study, where no significant correlation was reported between ROS production and sperm motility [26].…”