The Power of Gene Technologies: 1001 Ways to Create a Cell Model

Abstract

Modern society faces many biomedical challenges that require urgent solutions. Two of the most important include the elucidation of mechanisms of socially significant diseases and the development of prospective drug treatments for these diseases. Experimental cell models are a convenient tool for addressing many of these problems. The power of cell models is further enhanced when combined with gene technologies, which allows the examination of even more subtle changes within the structure of the genome and permits testing of proteins in a native environment. The list and possibilities of these recently emerging technologies are truly colossal, which requires a rethink of a number of approaches for obtaining experimental cell models. In this review, we analyze the possibilities and limitations of promising gene technologies for obtaining cell models, and also give recommendations on the development and creation of relevant models. In our opinion, this review will be useful for novice cell biologists, as it provides some reference points in the rapidly growing universe of gene and cell technologies.

Novel missense variants in brain morphogenic genes associated with depression and schizophrenia

“…However, the data we have obtained are insufficient to fully elucidate the mechanisms by which these identified genetic variants affect brain development and function, as well as their contributions to the pathogenesis of psychiatric and cognitive disorders. Unraveling these mechanisms will require additional studies using cellular and animal models, along with genetic technologies ( 75 – 77 ). These future investigations will serve as a continuation of the present study.…”

Morphogenetic theory of mental and cognitive disorders: the role of neurotrophic and guidance molecules

“…For instance, antidepressants that enhance neurotrophic factor production or anxiolytics that suppress neuroinflammation can be prescribed, combining the effects of 10.3389/fnmol.2024.1361764 Frontiers in Molecular Neuroscience 14 frontiersin.org symptomatic and pathogenetic therapies (Nibuya et al, 1995;Zheng et al, 2023). However, elucidating the contribution and functional significance of specific genes and their variants in neural tissue development warrants further investigation using cellular or animal models, which is now becoming increasingly accessible due to the possibilities of modern genetic and cellular technologies (Białoń and Wąsik, 2022;Dougnon and Matsui, 2022;Karagyaur et al, 2022).…”

Идентификация Геномных Вариантов В Генах Морфогенеза Нервной Ткани, Ассоциированных С Развитием Параноидной Шизофрении (На Материале Российской Популяции)

“…ЗАКЛЮЧЕНИЕ В данной работе впервые на материале российской популяции был идентифицирован ряд геномных миссенс-вариантов в генах морфогенеза головного мозга, ассоциированных с частотой встречаемости параноидной шизофрении. Для установления механизмов влияния выявленных геномных вариантов на процессы развития и функционирования головного мозга, а также определения их вклада в патогенез психических и когнитивных нарушений требуется проведение дополнительных исследований на клеточных и животных моделях с привлечением генетических технологий [23,24], что и будет осуществлено как самостоятельный фрагмент продолжения данного исследования.…”