The Potential Use of Dunaliella for the Production of Glycerol, β-Carotene and High-Protein Feed

Amotz, Ami Ben; Avron, Mordhay

doi:10.1007/978-1-4899-4998-1_17

Cited by 10 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As also found by others (e.g. Mil 'ko, 1963;Loeblich, 1982;Ben-Amotz & Avron, 1982) the highest carotenoid levels are found at the highest salinity and the steady-state carotenoid content reached is dependent upon the final salinity, irrespective of the magnitude of the salinity change.…”

Section: Discussionsupporting

confidence: 63%

Effects of salinity increase on carotenoid accumulation in the green alga Dunaliella salina

1990

View full text Add to dashboard Cite

The effect of sudden salinity increases on the kinetics of growth and carotenogenesis was studied in three geographically diverse isolates of Dunaliella salina. A sudden increase in salinity results in a lag phase in growth and the length of this lag phase is dependent on the final salinity and the magnitude of the salinity change (no lag at 10-15% w/v NaCI, 4-day lag at 30% NaCl). There is also a lag before an increase in the total carotenoid content can be measured following the salinity up-shock, and the length of the lag depends largely on the initial salinity and the magnitude of the salinity up-shock, whereas the rate of carotenogenesis and the final carotenoid content reached depend on the final salinity. The increase in total carotenoid content is mainly due to fl-carotene. Following the salinity up-shock (especially from 10% to 20% NaCl) the proportion of lutein as a percentage of total carotenoids decreases, whereas zeaxanthin increases. This suggests that the pathway synthesising lutein is more sensitive to salt or osmotic stress and is inhibited at higher salinities, thus leading to fl-carotene formation. The proportion of a-carotene does not change.

show abstract

Section: Discussionsupporting

confidence: 63%

Effects of salinity increase on carotenoid accumulation in the green alga Dunaliella salina

1990

View full text Add to dashboard Cite

show abstract

“…have been explored for industrial scale production in outdoor cultivation facilities for a variety of value-added and antioxidant compounds used in the neutraceutical, cosmetic, and biochemical industries (Borowitzka 1986 ). In particular, this genus serves as a potential biological source of food dyes, vitamin A, tocopherols, glycerol, lutein, peptides, and beta-carotene (Ben-Amotz and Avron 1982 ; Jin and Melis 2003 ; Lewin 1992 ). Furthermore, Dunaliella and related species have lipid and fatty acid compositions that are suitable for biodiesel production (Azachi et al 2002 ; McCormick et al 2006 ; Peeler et al 1989 ; Tornabene et al 1980 ), although the potential for exploiting this feedstock for biofuels application has been only marginally investigated.…”

Section: Introductionmentioning

confidence: 99%

The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

et al. 2014

View full text Add to dashboard Cite

Although great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, the role of interactions among these variables is largely nebulous. Here, we describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopic and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. This phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.

show abstract

“…are grown as a food source in aquaculture ( Davis & Guillard 1958; Spectorova et al . 1982 ) and D. salina is the richest algae source of β‐carotene and glycerol ( Ben‐Amotz & Avron 1982).…”

mentioning

confidence: 99%

Molecular identification of Dunaliella sp. utilizing the 18S rDNA gene

Olmos

Paniagua

Contreras

2000

Lett Appl Microbiol

View full text Add to dashboard Cite

J. OLMOS, J. PANIAGUA and R. CONTRERAS.2000.The utilization of micro‐algae for the production of food and fine chemicals is growing in importance. However, confusion of names and species makes comparison of results by different authors very difficult. In this work, five species of Dunaliella were characterized using their 18S ribosomal RNA genes. Conserved oligonucleotides complementary to 5′ and 3′ termini of the 18S rDNAs were designed and utilized to amplify theme, restriction fragment length polymorphism analysis of the polymerase chain reaction products was developed. Species‐specific primers were also designed and utilized to corroborate the identification.

show abstract

The Potential Use of Dunaliella for the Production of Glycerol, β-Carotene and High-Protein Feed

Cited by 10 publications

References 10 publications

Effects of salinity increase on carotenoid accumulation in the green alga Dunaliella salina

Effects of salinity increase on carotenoid accumulation in the green alga Dunaliella salina

The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

Molecular identification of Dunaliella sp. utilizing the 18S rDNA gene

Contact Info

Product

Resources

About