Molecular identification of Dunaliella sp. utilizing the 18S rDNA gene

Olmos, Jorge; Paniagua, Juan Daniel Carpio; Contreras, Rosalía

doi:10.1046/j.1472-765x.2000.00672.x

Cited by 63 publications

(77 citation statements)

References 11 publications

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“…The 18S rDNA gene is commonly used for the identification of micro-eukaryotes providing accurate results [14,15]. The internal transcribed spacer 2 (ITS2) and the adjacent partial sequences of the 5.8S and 28S improved the final genetic analysis, providing additional confirmatory data.…”

Section: Identification Of Strains By 18s Rdna Sequencingmentioning

confidence: 99%

Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production

et al. 2013

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Abstract:The isolation of lipid-rich autochthonous strains of microalgae is a crucial stage for the development of a microalgae-based biofuel production plant, as these microalgae already have the necessary adaptations to withstand competition, predation and the temperatures observed at each production site. This is particularly important in extreme climates such as in Saudi Arabia. Resorting to fluorescence activated cell sorting (FACS) we screened for and isolated several microalgal strains from samples collected from the Red Sea. Relying on the fluorescence of BODIPY 505/515 (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diazasindacene) and growth performance, four promising candidates were identified and the total lipid content and fatty acid profile was assessed for biofuels production. Selected isolates were classified as chlorophytes, belonging to three different genera: Picochlorum, Nannochloris and Desmochloris. The lipid contents were assessed microscopically by means of BODIPY 505/515-associated fluorescence to detect OPEN ACCESSEnergies 2013, 6 2774 intracellular lipid bodies, which revealed several lipid drops in all selected strains. This result was confirmed by lipid gravimetric determination, which demonstrated that all strains under study presented inner cell lipid contents ranging from 20% to 25% of the biomass dry weight. Furthermore, the fatty acid methyl esters profile of all strains seems ideal for biodiesel production due to a low degree of polyunsaturated fatty acid methyl esters and high amount of palmitic and oleic acids.

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Section: Identification Of Strains By 18s Rdna Sequencingmentioning

confidence: 99%

Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production

et al. 2013

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“…Finally, two-week old colonies were carefully picked and inoculated into 100 ml of fresh Artificial Sea Water (ASW) medium in 250 ml flasks. This pure culture was maintained and sub-cultured every fortnight to reach a log phase as described in the previous studies [10,[19][20][21][22][23][24][25]. Non-stress and stress phases Isolates were placed in culture media containing 1.5M NaCl concentration for 30 days.…”

Section: Resultsmentioning

confidence: 99%

Effect of Different Salinity Levels on β-Carotene Production by Dunaliella Sp. Isolates from the Maharlu Lake, Iran

Mohammadi¹,

Arashrad²

2016

mljgoums

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Background and Objective: Microalgae are a group of algae that produce biochemical products consisting of a wide range of carbohydrates, lipids and proteins that are commercially valuable. Interest in microalgal cultivation is currently blossoming globally. Species of Dunaliella are found in freshwater, euryhaline habitats of all continents, oceans including the Dead Sea and even the salt lakes of the Antarctic. This study investigates the effect of different salinity levels on β-carotene production by Dunaliella sp.Methods: Water samples from a hyper-saline lake (the Maharlu Lake in Shiraz) were cultured in modified Johnson media. The β-carotene content was measured after the samples were treated with different salinities (1, 2 and 3M NaCl).Results: The cell count and β-carotene content of Dunaliella sp. samples ranged between 0.46×10 6 to 2.12×10 6 cell.mL -1 and 0.15 to 9.98 pg.cell -1 , respectively. At the end of the experiments, the mean maximum cell content (1.78×10 6 cell. mL -1 ) and the highest mean β-carotene content (7.41 pg. cell -1 ) were obtained at 2 and 3M NaCl concentrations, respectively.Conclusion: Salinity of the medium might affect the quantity and composition of carotenoids in Dunaliella sp. isolates. Alteration of the culture medium's salinity to 3M NaCl significantly increases the accumulation of β-carotene and total carotenoids in Dunaliella sp. isolates.

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“…Although, the only way to know the identity of each strain for certain would be to perform genomic analysis of the 18S rRNA. This technique has been established for many species of Dunaliella, including both the UTEX 1664 and CCAP 19/18 strains (Olmos et al, 2000;Polle et al, 2008). Due to our inability to construct Dunaliella-specific expression vectors, the attempts to genetically transform D. salina were limited to the use of the C. reinhardtii bleomycinresistance plasmid, pSP124.…”

Section: Discussionmentioning

confidence: 99%

Paving the Road to Algal Biofuels with the Development of a Genetic Infrastructure

Rosenberg¹,

Betenbaugh²,

Oyler³

2011

Biofuel's Engineering Process Technology

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Molecular identification of Dunaliella sp. utilizing the 18S rDNA gene

Cited by 63 publications

References 11 publications

Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production

Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production

Effect of Different Salinity Levels on β-Carotene Production by Dunaliella Sp. Isolates from the Maharlu Lake, Iran

Paving the Road to Algal Biofuels with the Development of a Genetic Infrastructure

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