The potential to improve cell infiltration in composite fiber-aligned electrospun scaffolds by the selective removal of sacrificial fibers

Baker, Brendon M.; Gee, Albert O.; Metter, Robert B.; Nathan, Ashwin S.; Marklein, Ross A.; Burdick, Jason A.; Mauck, Robert L.

doi:10.1016/j.biomaterials.2008.01.032

Cited by 566 publications

(519 citation statements)

References 35 publications

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“…It is well documented that changes is fibre diameter and orientation induce a response of the cells in many tissue types (Yan et al 2012;Balguid et al 2009;Wang et al 2012), but the effect on renal cells has been ignored until now. The diameter of the fibre determines the pore size of the scaffold, and this determines how well cells will penetrate into a scaffold (Balguid et al 2009;Baker et al 2008;Ju et al 2010). Techniques such as cryogenic electrospinning have shown to create structures with a much greater porosity, enabling cell infiltration throughout the scaffold (Leong et al 2013;Bulysheva et al 2013;Leong et al 2009); this can be controlled by modifying the humidity of the spinning environment, changing the amount of ice crystal formation on the cooled mandrel (Leong et al 2013).…”

Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…63 A dual electrospinning setup has been created with the additional stream of polymer serving to create a sacrificial fiber that is eluted after spinning, increasing the void space in the construct. 64 Although these strategies have successfully increased scaffold pore size, the mechanical strength of the constructs was reduced. 62 Recently, it was…”

Section: 54mentioning

confidence: 99%

Polymeric Nanofibers in Tissue Engineering

Dahlin

Kasper

Mikos

2011

Tissue Engineering Part B: Reviews

288

198

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Polymeric nanofibers can be produced using methods such as electrospinning, phase separation, and selfassembly, and the fiber composition, diameter, alignment, degradation, and mechanical properties can be tailored to the intended application. Nanofibers possess unique advantages for tissue engineering. The small diameter closely matches that of extracellular matrix fibers, and the relatively large surface area is beneficial for cell attachment and bioactive factor loading. This review will update the reader on the aspects of nanofiber fabrication and characterization important to tissue engineering, including control of porous structure, cell infiltration, and fiber degradation. Bioactive factor loading will be discussed with specific relevance to tissue engineering. Finally, applications of polymeric nanofibers in the fields of bone, cartilage, ligament and tendon, cardiovascular, and neural tissue engineering will be reviewed.

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“…29,[86][87][88] These systems may be used to create composite scaffolds (with a different polymer in each jet) whose properties reflect the properties and ratios of the individual components. [89][90][91][92][93][94] Several such multicomponent scaffolds are described in detail below.…”

Section: Mechanical Properties Of Nanofibrous Assembliesmentioning

confidence: 99%

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Mauck

Baker

Nerurkar

et al. 2009

Tissue Engineering Part B: Reviews

Self Cite

186

177

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Tissue engineering of fibrous tissues of the musculoskeletal system represents a considerable challenge because of the complex architecture and mechanical properties of the component structures. Natural healing processes in these dense tissues are limited as a result of the mechanically challenging environment of the damaged tissue and the hypocellularity and avascular nature of the extracellular matrix. When healing does occur, the ordered structure of the native tissue is replaced with a disorganized fibrous scar with inferior mechanical properties, engendering sites that are prone to re-injury. To address the engineering of such tissues, we and others have adopted a structurally motivated approach based on organized nanofibrous assemblies. These scaffolds are composed of ultrafine polymeric fibers that can be fabricated in such a way to recreate the structural anisotropy typical of fiber-reinforced tissues. This straight-and-narrow topography not only provides tailored mechanical properties, but also serves as a 3D biomimetic micropattern for directed tissue formation. This review describes the underlying technology of nanofiber production and focuses specifically on the mechanical evaluation and theoretical modeling of these structures as it relates to native tissue structure and function. Applying the same mechanical framework for understanding native and engineered fiber-reinforced tissues provides a functional method for evaluating the utility and maturation of these unique engineered constructs. We further describe several case examples where these principles have been put to test, and discuss the remaining challenges and opportunities in forwarding this technology toward clinical implementation.

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The potential to improve cell infiltration in composite fiber-aligned electrospun scaffolds by the selective removal of sacrificial fibers

Cited by 566 publications

References 35 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Polymeric Nanofibers in Tissue Engineering

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

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