The potential role of mast cells and fibroblast growth factor-2 in the development of hypertension-induced renal damage

Stanchev, Stancho; Landzhov, Boycho; Kotov, Georgi; Stamenov, Nikola; Dikov, Tihomir; Iliev, Alexandar

doi:10.1016/j.acthis.2020.151599

Cited by 6 publications

(8 citation statements)

References 41 publications

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“…Three randomly selected rats from each group were sacrifi ced and their kidneys were obtained for analysis according to the previously described standardised procedure (24).…”

Section: Kidneymentioning

confidence: 99%

“…Slides from the heart and kidney were processed for routine light microscopic study in the manner previously described (23,24).…”

Section: Light Microscopymentioning

confidence: 99%

“…The immunohistochemical study was conducted through the heat-induced epitope retrieval (HIER) technique as previously described (24). We used mouse monoclonal anti-VEGF-A IgG antibody (Santa Cruz Biotechnology Catalogue No.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…sc-516102) at concentration 1:75 for 2 h was performed. All other procedures followed the standardised protocol described in our previous work (23,24).…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Depletion of vascular adaptive mechanisms in hypertension-induced injury of the heart and kidney

Stanchev¹,

Kotov²,

Landzhov³

et al. 2023

BLL

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BACKGROUND: Vascular endothelial growth factor (VEGF) is a signalling protein of critical importance for angiogenesis. In an effort to better understand its signifi cance in hypertension-induced injury of the heart and kidney we aimed at studying the changes in its expression in an experimental model and correlated it with capillary density in the myocardium and the renal parenchyma. METHODS: We used two age groups of spontaneously hypertensive rats (6-and 12-month-old), indicative of early and advanced hypertension. VEGF expression was assessed and a semi-quantitative analysis of its immunoreactivity was conducted. Changes in capillary density in the myocardium and kidney were assessed for statistical signifi cance and correlations with VEGF expression were established. RESULTS: We reported stronger VEGF expression in animals with early compared to advanced hypertension in all examined structures. Capillary density decreased signifi cantly at age 12 months compared to 6 months and was signifi cant in all examined structures. A positive correlation was established between capillary density and the expression of VEGF. CONCLUSION: These fi ndings underscore the key signifi cance of VEGF for compensatory angiogenesis and suggest that a statistically signifi cant depletion of these vascular adaptive mechanisms is a major aspect in the cascade of hypertension-induced injury of the heart and kidney (Tab. 3, Fig. 26, Ref. 47).

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“…Three randomly selected rats from each group were sacrifi ced and their kidneys were obtained for analysis according to the previously described standardised procedure (24).…”

Section: Kidneymentioning

confidence: 99%

“…Slides from the heart and kidney were processed for routine light microscopic study in the manner previously described (23,24).…”

Section: Light Microscopymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

“…sc-516102) at concentration 1:75 for 2 h was performed. All other procedures followed the standardised protocol described in our previous work (23,24).…”

Section: Immunohistochemistrymentioning

confidence: 99%

See 2 more Smart Citations

Depletion of vascular adaptive mechanisms in hypertension-induced injury of the heart and kidney

Stanchev¹,

Kotov²,

Landzhov³

et al. 2023

BLL

View full text Add to dashboard Cite

show abstract

“…Sections. After dewaxing and dehydrating, paraffin sections (4 μm) were stained by the stain kits of hematoxylin-eosin (H&E), periodic acid-Schiff (PAS), and toluidine blue (TB) stains according to the routine steps [19][20][21]. Moreover, the sections were dehydrated again, and then, they were mounted with neutral gum and examined by microscopy (ECLIPSE E100, Nikon, Japan).…”

Section: Staining Analysis Of Tissuementioning

confidence: 99%

The Effects of Saposhnikovia divaricata Aqueous Extracts on the Inflammation and Intestinal Microflora in Allergic Rhinitis Mice

Chen

Wang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Allergic rhinitis (AR) is a type I allergic disease induced by IgE. Traditional Chinese medicine Saposhnikovia divaricata (Turcz.) Schischk (SD) has anti-inflammatory and antiallergic effects. Materials and Methods. AR model mice were constructed with ovalbumin (OVA) sensitization to observe the improving effect of SD treatment on AR by counting the number of sneezing and rubbing the nose, hematoxylin-eosin, periodic acid-Schiff, and toluidine blue stains. In addition, the allergy and inflammatory cytokines levels and inflammatory cells were observed by ELISA and Wright’s-Giemsa stain. The protein levels of the TLR4/TRAF6/NF-κB and IL-6/ROR-γt/STAT3 pathways were measured by immunohistochemistry, quantitative real-time PCR, and western blot. The intestinal microflora abundance in mice was observed by 16S rDNA high-throughput sequencing. Results. SD treatment inhibited the sneezing and rubbing times of the nose, decreased the degree of a dense arrangement of cells and mucosal swelling and the number of goblet and mast cells of nasal lavage fluid, reduced the levels of IgE, histamine, Leukotriene B4, IL-4, IL-5, TNF-α, IL-6, and IL-17, the eosinophils, neutrophils, and lymphocytes number, the LR4, TRAF6, IL-6, ROR-γt, and STAT3 mRNA levels, respectively, while, it increased the IL-2, IL-10, IFN-γ, and TGF-β1 proteins. SD treatment inhibited the NF-κB, p-STAT3, TLR4, TRAF6, and p-IκBα/IκBα proteins. Besides, the effects of OVA and SD treatments were significantly correlated with the abundance of intestinal microflora. The abundances of Cytophagales, Burkholderia, Alteromonadales, Lactococcus, and Clostridiaceae were changed in SD treatment on AR mice. Conclusions. This study provides a possibility that the improvement effect of SD treatment on allergies and inflammation in AR mice may be related to the TLR4/TRAF6/NF-κB and IL-6/ROR-γt/STAT3 pathways and intestinal microflora modulation.

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Adefovir accumulation in the renal interstitium triggers mast cell degranulation and promotes renal interstitial fibrosis

et al. 2022

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The potential role of mast cells and fibroblast growth factor-2 in the development of hypertension-induced renal damage

Cited by 6 publications

References 41 publications

Depletion of vascular adaptive mechanisms in hypertension-induced injury of the heart and kidney

Depletion of vascular adaptive mechanisms in hypertension-induced injury of the heart and kidney

The Effects of Saposhnikovia divaricata Aqueous Extracts on the Inflammation and Intestinal Microflora in Allergic Rhinitis Mice

Adefovir accumulation in the renal interstitium triggers mast cell degranulation and promotes renal interstitial fibrosis

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