The potential role of HCV core antigen testing in diagnosing HCV infection

Dawson, George J.

doi:10.3851/imp2463

Cited by 18 publications

(16 citation statements)

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“…ARCHITECT HCV Ag assay (Abbott Diagnostics, Chicago, IL, USA) has emerged as a promising candidate that quantifies HCV in less than 60 min . This assay has not been previously evaluated in HCV genotype 4 in Egypt.…”

Section: Discussionmentioning

confidence: 99%

“…Hepatitis C core Ag assay has emerged as a marker of HCV infection and an indirect marker of HCV replication . HCV core Ag is detected by several techniques including enzyme‐linked immunosorbent assays (ELISAs), automated chemiluminescent immunoassay (CLIA) . The automated Abbott ARCHITECT HCV Ag assay (Wiesbaden, Germany) is a two‐step chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of HCV core antigen in serum and plasma .…”

Section: Introductionmentioning

confidence: 99%

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The accuracy and cost‐effectiveness of hepatitis C core antigen assay in the monitoring of anti‐viral therapy in patients with chronic hepatitis C genotype 4

Kamal

Kassim

Gohary

et al. 2015

Aliment Pharmacol Ther

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The accuracy and cost‐effectiveness of hepatitis C core antigen assay in the monitoring of anti‐viral therapy in patients with chronic hepatitis C genotype 4

Kamal

Kassim

Gohary

et al. 2015

Aliment Pharmacol Ther

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“…Moreover, the HCV core antigen test can be utilized to detect the early phase of HCV infection prior to the development of antibodies, both in the blood bank setting and in the diagnostic laboratory (22). However, it is difficult to generate high-quality antibodies against core protein, although detection of HCV core present in serum is highly sensitive and specific (3).…”

Section: Figmentioning

confidence: 99%

Inhibition of Hepatitis C Virus Production by Aptamers against the Core Protein

Shi

Gao

et al. 2014

J Virol

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bHepatitis C virus (HCV) core protein is essential for virus assembly. HCV core protein was expressed and purified. Aptamers against core protein were raised through the selective evolution of ligands by the exponential enrichment approach. Detection of HCV infection by core aptamers and the antiviral activities of aptamers were characterized. The mechanism of their anti-HCV activity was determined. The data showed that selected aptamers against core specifically recognize the recombinant core protein but also can detect serum samples from hepatitis C patients. Aptamers have no effect on HCV RNA replication in the infectious cell culture system. However, the aptamers inhibit the production of infectious virus particles. Beta interferon (IFN-␤) and interferon-stimulated genes (ISGs) are not induced in virally infected hepatocytes by aptamers. Domains I and II of core protein are involved in the inhibition of infectious virus production by the aptamers. V31A within core is the major resistance mutation identified. Further study shows that the aptamers disrupt the localization of core with lipid droplets and NS5A and perturb the association of core protein with viral RNA. The data suggest that aptamers against HCV core protein inhibit infectious virus production by disrupting the localization of core with lipid droplets and NS5A and preventing the association of core protein with viral RNA. The aptamers for core protein may be used to understand the mechanisms of virus assembly. Core-specific aptamers may hold promise for development as early diagnostic reagents and potential therapeutic agents for chronic hepatitis C.

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“…A number of studies have demonstrated good correlation between HCVcAg and HCV RNA levels, particularly in HCV-monoinfected individuals [6,7,8,9,10,11,12,13]. As a result, HCVcAg testing is considered to be an alternative assay to conventional HCV RNA quantification with the advantages of rapidity, reproducibility and feasibility in settings with limited resources [14].…”

Section: Introductionmentioning

confidence: 99%

The Correlation between Hepatitis C Core Antigen and Hepatitis C Virus RNA Levels with Respect to Human Immunodeficiency Virus Status, Hepatitis C Virus Genotype and Interferon-Lambda-4 Polymorphism

Thông¹,

Akkarathamrongsin²,

Avihingsanon³

et al. 2015

Intervirology

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Objectives: Serum hepatitis C virus (HCV) core antigen (HCVcAg) concentrations correlate with HCV RNA levels in HCV monoinfected patients. Data in HCV/HIV coinfected patients are still limited. We aim to compare the use of HCVcAg measurement with respect to HIV status, HCV genotypes, interferon-lambda-4 (IFNL4) polymorphism and clinical parameters. Methods: We analyzed an untreated cohort of 104 patients with HCV monoinfection and 85 patients with HCV/HIV coinfection. Serum HCVcAg was measured by a commercial chemiluminescent microparticle immunoassay. The presence of IFNL4 polymorphism ss469415590 was identified by real-time PCR. Results: log₁₀ HCVcAg levels were significantly correlated with corresponding log₁₀ HCV RNA levels (r = 0.889, p < 0.001), but not with ALT levels and liver stiffness. The correlation between HCV RNA and HCVcAg was particularly high in coinfected patients and those with high viremia. Mean log₁₀ HCVcAg concentration was significantly higher in coinfected patients than in monoinfected patients. Patients harboring the TT/TT genotype of ss469415590 had significantly higher levels of log₁₀ HCVcAg than those with the non-TT/TT genotype. HCVcAg levels were similar across HCV genotypes. Conclusions: HCVcAg concentrations had an excellent correlation with HCV RNA levels, particularly in HCV/HIV-coinfected individuals and might be associated with IFNL4 polymorphism. HCVcAg testing could be used as an alternative to HCV RNA assays in resource-limited settings.

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The potential role of HCV core antigen testing in diagnosing HCV infection

Cited by 18 publications

References 0 publications

The accuracy and cost‐effectiveness of hepatitis C core antigen assay in the monitoring of anti‐viral therapy in patients with chronic hepatitis C genotype 4

The accuracy and cost‐effectiveness of hepatitis C core antigen assay in the monitoring of anti‐viral therapy in patients with chronic hepatitis C genotype 4

Inhibition of Hepatitis C Virus Production by Aptamers against the Core Protein

The Correlation between Hepatitis C Core Antigen and Hepatitis C Virus RNA Levels with Respect to Human Immunodeficiency Virus Status, Hepatitis C Virus Genotype and Interferon-Lambda-4 Polymorphism

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