The Potential of Rice Bran and Chito-Oligosaccharide as Natural Prebiotic on Traditional Tempe in Indonesia

Harti, Agnes Sri; Nurhidayati, Anis; Handayani, Desy

doi:10.7763/ijbbb.2013.v3.296

Cited by 3 publications

(5 citation statements)

References 9 publications

(5 reference statements)

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“…The functional groups of chitosan (primary hydroxyl groups in C-6, secondary hydroxyl groups in C-3 and amino groups in C-2) cause the chitosan to be chemically modified. Chitosan had been widely used in biomedical and pharmaceutical fields because it was biodegradable, biocompatible, and non-toxic [9].…”

Section: Resultsmentioning

confidence: 99%

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The Effectiveness of Snail Mucus (Achatina Fulica) and Chitosan Toward Limfosit Proliferation in Vitro

Harti

Murharyati

et al. 2018

Asian J Pharm Clin Res

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Objective: The objective of this research was to assess the effectiveness of mucous and chitin slime cream against lymphocyte proliferation in vitro.Methods: The research methods include snail mucus isolation, snail mucus cream material preparation, and lymphocyte cell proliferation test of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method.Results: Lymphocyte cell proliferation test of MTT reduction method showed that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream. The 5% snail mucus cream 5% provides a higher proliferative activity than the 100% snail mucus. Positive control using Con A solution. Negative control was treatment without addition of test solution. The differences in lymphocyte proliferation activity were due to the presence of active compounds in chitosan, snail mucus, and snail slime cream that potentially increase lymphocyte proliferation. Significant differences in lymphocyte cell proliferation as Group K1, K2, K3, K4, K5, and K6.Conclusion: The effectiveness of snail and chitosan slime cream toward lymphocyte proliferation by in vitro that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream.

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Section: Resultsmentioning

confidence: 99%

“…Chitosan is a chitin derived compound that can be biologically degraded, non-toxic, non-immunogenic, and biologically compatible with animal body tissues. Chitosan has been widely used in biomedical and pharmaceutical fields because of its biodegradable, biocompatible, and non-toxic [2].…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of Snail Mucus (Achatina Fulica) and Chitosan Toward Limfosit Proliferation in Vitro

Harti

Murharyati

et al. 2018

Asian J Pharm Clin Res

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“…Chito-oligosaccharide, which can protect and lessen the growth rate of pathogenic bacteria, is synthesised from chitin deacetylation from crab shells in Indonesia. It is used to produce functional food, such as symbiotic tofu as a prebiotic and natural preservative (Harti, 2015). Chitosan, a derivative of chitin in crab shells, can serve as a prebiotic in metabolic effects and effects on the gut microbiota composition (Lopez-Santamarina et al, 2020).…”

Section: Value Creation Opportunity For Crab Shells In Thailandmentioning

confidence: 99%

The value chain analysis and the effect of COVID-19 on a small-sized blue crab manufacturer: A case study in Thailand

2022

KJSS

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Blue crab is a high-value raw material and can be sold as a fresh raw material in both domestic and international markets. Unfortunately, during the COVID-19 crisis, the demand has decreased dramatically. The objective of this study was to investigate the value chain of blue crab for a small enterprise that processed blue-crab-based food in Surat Thani province, Thailand. The related stakeholders and their roles were identified. Next, the transactions of the manufacturer before and during the COVID-19 crisis were compared. The Integration Definition for Functional Modeling (IDEF0) was implemented to analyse the supply chain of products from blue crabs. The enterprise sales volume decreased by 60 percent during the crisis, and some customers could not pay with cash. The proportion of customers via an online platform and social media increased. There are eight components used in different products, with finger and fat being about 7.29 percent of the boiled crab weight, with a value lower than that of other components. In addition, the crab shell, which is about 43.89 percent of the raw crab weight, was thrown away. Consequently, the enterprise developed various products using these components and maximised the profit by determining which parts of the crab could be used to produce products to satisfy customers. Furthermore, the company should implement proper supply chain management. In the future, the government and universities can research how to utilise crab shells. This research could be a model for other SMEs to create more valuable products in order to survive during the crisis.

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“…Chitosan is β-(1.4)-2 amino-2 deoxy D-glucopyranose as a product of chitin deacetylation. The uniqueness of chitosan is polycationic so that it can suppress the growth rate of diarrheagenic Escherichia coli in vitro [5]. Synbiotic (Chitooligosaccharide and Lactobacillus acidophilus) bio preparation in yogurt can reduce cholesterol levels in vitro and in vivo [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…The uniqueness of chitosan is polycationic so that it can suppress the growth rate of diarrheagenic Escherichia coli in vitro [5]. Synbiotic (Chitooligosaccharide and Lactobacillus acidophilus) bio preparation in yogurt can reduce cholesterol levels in vitro and in vivo [6,7]. Chitosan has been widely used in biomedical and pharmaceutical fields because it is biodegradable, non-toxic, non-immunogenic, and biocompatible with animal body tissues [8].…”

Section: Introductionmentioning

confidence: 99%

The Sensitivity Test of Mycobacterium Tuberculosis Isolates From Suspect Tuberculosis Patients to the Seromucous of Snail and Chitosan as an Alternative Anti-Tuberculosis Drugs

Sutanto

Harti

et al. 2020

Asian J Pharm Clin Res

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Objective: The purpose of this research is to study the sensitivity of Mycobacterium tuberculosis (MTB) isolates from suspect TB patients to seromucous of snail and chitosan as an alternative to anti-TB drugs. Methods: The research methods include management specimen, freeze-drying of snail seromucous; formulation of dosage preparation; identification of MTB isolates; and sensitivity testing of MTB isolates to snail seromucous, chitosan, and streptomycin, isoniazid, rifampicin, and ethambutol (SIRE). Results: The characteristics of respondents by sex and age are the majority of male respondents and productive adult age that is 26 years–52 years. MTB isolates used in the study were obtained from the results of the screening of sputum samples of suspect TB patients through microscopic smear examination and molecular rapid test using GeneXpert tools. MTB isolates in patients suspect TB are resistant against seromucous of snails and chitosan that it is compared with SIRE. The dosage of snail seromucous is 8000 mg/l, chitosan 2% is 800 mg/l, and SIRE, respectively (rifampicin 8000 mg/l, isoniazid 20 mg/l, ethambutol 200 mg/l, streptomycin 800 mg/l). Conclusion: MTB isolates from patients suspect who TB is resistant to seromucous of snail (8000 mg/l) and chitosan (800 mg/l).

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The Potential of Rice Bran and Chito-Oligosaccharide as Natural Prebiotic on Traditional Tempe in Indonesia

Cited by 3 publications

References 9 publications

The Effectiveness of Snail Mucus (Achatina Fulica) and Chitosan Toward Limfosit Proliferation in Vitro

The Effectiveness of Snail Mucus (Achatina Fulica) and Chitosan Toward Limfosit Proliferation in Vitro

The value chain analysis and the effect of COVID-19 on a small-sized blue crab manufacturer: A case study in Thailand

The Sensitivity Test of Mycobacterium Tuberculosis Isolates From Suspect Tuberculosis Patients to the Seromucous of Snail and Chitosan as an Alternative Anti-Tuberculosis Drugs

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