The potential of mucoadhesive polymer in enhancing efficacy of direct immersion vaccination against Flavobacterium columnare infection in tilapia

Kitiyodom, Sirikorn; Kaewmalun, Somrudee; Nittayasut, Naiyaphat; Suktham, Kunat; Surassmo, Suvimol; Namdee, Katawut; Rodkhum, Channarong; Pirarat, Nopadon; Yata, Teerapong

doi:10.1016/j.fsi.2018.12.005

Cited by 36 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At 30 and 60 days after vaccination, the fish were immersion challenged with 1 × 10 6 CFU mL −1 of a virulent strain of F. columnare for 1 h. Cumulative mortality was recorded for 10 days after immersion challenge. At 30 days post-vaccination with naked and chitosan vaccines, RPS was 4% and 81%, respectively [17], and mortality of non-vaccinated and naked vaccine groups was 90% and 87%, versus 17% mortality in the chitosan vaccine group. Sixty days after vaccination, the RPS was reduced to 58.…”

Section: Columnarismentioning

confidence: 93%

“…A mucoadhesive vaccine delivery system to enhance the efficacy of direct immersion vaccination against F. columnare, the causative agent of columnaris disease was tested in red tilapia [17]. A formalin-killed negatively charged bacterial cell suspension was used to prepare a mucoadhesive vaccine by electrostatic coating with positively charged chitosan.…”

Section: Columnarismentioning

confidence: 99%

“…The first one described increased the adhesion and uptake of antigens of inactivated Flavobacterium when coated by positively charged chitosan which displayed mucoadhesive properties. This modality increased vaccine efficacy compared to what was obtained using naked vaccine antigens [17]. The other one used TNF alpha (TNF-α) nanoparticles which hold promise as an adjuvant for immersion vaccination [4].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Review on Immersion Vaccines for Fish: An Update 2019

Bøgwald

Dalmo

2019

Microorganisms

View full text Add to dashboard Cite

Immersion vaccines are used for a variety of aquacultured fish to protect against infectious diseases caused by bacteria and viruses. During immersion vaccination the antigens are taken up by the skin, gills or gut and processed by the immune system, where the resulting response may lead to protection. The lack of classical secondary responses following repeated immersion vaccination may partly be explained by the limited uptake of antigens by immersion compared to injection. Administration of vaccines depends on the size of the fish. In most cases, immersion vaccination is inferior to injection vaccination with regard to achieved protection. However, injection is problematic in small fish, and fry as small as 0.5 gram may be immersion vaccinated when they are considered adaptively immunocompetent. Inactivated vaccines are, in many cases, weakly immunogenic, resulting in low protection after immersion vaccination. Therefore, during recent years, several studies have focused on different ways to augment the efficacy of these vaccines. Examples are booster vaccination, administration of immunostimulants/adjuvants, pretreatment with low frequency ultrasound, use of live attenuated and DNA vaccines, preincubation in hyperosmotic solutions, percutaneous application of a multiple puncture instrument and application of more suitable inactivation chemicals. Electrostatic coating with positively charged chitosan to obtain mucoadhesive vaccines and a more efficient delivery of inactivated vaccines has also been successful.

show abstract

Section: Columnarismentioning

confidence: 93%

Section: Columnarismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Review on Immersion Vaccines for Fish: An Update 2019

Bøgwald

Dalmo

2019

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Moreover, the nasal route of drug delivery includes both local and systemic drug delivery. A wide range of pharmaceutical dosage forms, including solutions, gels, suspensions, emulsions, liposomes, and microparticles administered via nasal route (Jullaphant et al, 2009;Kitiyodom et al, 2019;Lengyel et al, 2019;Salade et al, 2019), achieved an enhanced targeting ability and reduced systemic side-effects.…”

Section: Advantages and Limitations Of Nasal Drug Delivery Routementioning

confidence: 99%

Design and Application in Delivery System of Intranasal Antidepressants

Tao

Wang

2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

One of the major reasons why depressed patients fail their treatment course is the existence of the blood-brain barrier (BBB), which prevents drugs from being delivered to the central nervous system (CNS). In recent years, nasal drug delivery has achieved better systemic bioavailability and activity in low doses in antidepressant treatment. In this review, we focused on the latest strategies for delivery carriers (or formation) of intranasal antidepressants. We began this review with an overview of the nasal drug delivery systems, including nasal drug delivery route, absorption mechanism, advantages, and limitations in the nasal drug delivery route. Next, we introduced the development of nasal drug delivery devices, such as powder devices, liquid-based devices, and so on. Finally, intranasal delivery carriers of antidepressants in clinical studies, including nanogels, nanostructured lipid, liposomes nanoparticles, nanoemulsions/microemulsion, were summarized. Moreover, challenges and future perspectives on recent progress of intranasal delivery carriers in antidepressant treatments were discussed.

show abstract

“…Nevertheless, this approach has been impeded by the fact that the effectiveness of antigen absorption by mucosal tissues is limited and the potency of induction of protective immune responses can be low and short in duration. Our previous study demonstrated the use of a biomimetic-mucoadhesive nanovaccine that allows better adsorption of antigens to the mucosal surfaces of fish [13,14]. Strong mucosal immunity was triggered by the vaccine, inducing an immune cascade at the mucosal site and in the mucosal associated lymphoid tissue (MALT) following immersion immunization [4].…”

Section: Introductionmentioning

confidence: 99%

Immersion Vaccination by a Biomimetic-Mucoadhesive Nanovaccine Induces Humoral Immune Response of Red Tilapia (Oreochromis sp.) against Flavobacterium columnare Challenge

et al. 2021

Self Cite

View full text Add to dashboard Cite

Immersion vaccination with a biomimetic mucoadhesive nanovaccine has been shown to induce a strong mucosal immune response against columnaris disease, a serious bacterial disease in farmed red tilapia caused by Flavobacterium columnare. However, the induction of a systemic immune response by the vaccine is yet to be investigated. Here, we examine if a specific humoral immune response is stimulated in tilapia by a biomimetic-mucoadhesive nanovaccine against Flavobacterium columnare using an indirect-enzyme-linked immunosorbent assay (ELISA), serum bactericidal activity (SBA) and the expression of immune-related genes within the head-kidney and spleen, together with assessing the relative percent survival of vaccinated fish after experimentally infecting them with F. columnare. The anti-IgM antibody titer of fish at 14 and 21 days post-vaccination was significantly higher in chitosan complex nanoemulsion (CS-NE) vaccinated fish compared to fish vaccinated with the formalin-killed vaccine or control fish, supporting the serum bactericidal activity results at these time points. The cumulative mortality of the unvaccinated control fish was 87% after challenging fish with the pathogen, while the cumulative mortality of the CS-NE vaccinated group was 24%, which was significantly lower than the formalin-killed vaccinated and control fish. There was a significant upregulation of IgM, IgT, TNF α, and IL1-β genes in the spleen and kidney of vaccinated fish. Significant upregulation of IgM and IgT genes was observed in the spleen of CS-NE vaccinated fish. The study confirmed the charged-chitosan-based mucoadhesive nanovaccine to be an effective platform for immersion vaccination of tilapia, with fish generating a humoral systemic immune response against columnaris disease in vaccinated fish.

show abstract

The potential of mucoadhesive polymer in enhancing efficacy of direct immersion vaccination against Flavobacterium columnare infection in tilapia

Cited by 36 publications

References 24 publications

Review on Immersion Vaccines for Fish: An Update 2019

Review on Immersion Vaccines for Fish: An Update 2019

Design and Application in Delivery System of Intranasal Antidepressants

Immersion Vaccination by a Biomimetic-Mucoadhesive Nanovaccine Induces Humoral Immune Response of Red Tilapia (Oreochromis sp.) against Flavobacterium columnare Challenge

Contact Info

Product

Resources

About