The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

Abstract

The present study was investigated the potential of lipolytic fungi (molds) isolated from landfill soil in degrading Poly-β-hydroxybutyrate (PHB). Screening of PHB-degrading lipolytic molds was done in two stages, such as screening of lipolytic molds which was identified by the formation of orange fluorescent halos around the colony on rhodamine B agar medium and the degradation PHB ability test was identified by the formation of clear zone around colony on PHB emulsion medium. Characterization of isolates was done based on phenotypic characters and the identification was done by numerical-phenetic analysis. Three lipolytic mold isolates that have ability in degrading PHB bioplastic i.e isolate KC1, KE1 and KE6. These molds have asexual spore form conidia, foot cell, septate hyphae, unbranched conidiophore, and spore mass located at the apex of phialid. The identification results showed that isolate KC1 is identic to Aspergillus terreus, KE1 is identic to Aspergillus niger and KE6 is identic to Aspergillus fumigatus.

Optimum Medium for Lipase Production by Lipolytic Filamentous Fungi Isolated from Kendari Landfill Soil

“…Aspergillus has been known as the lipase producer that is suitable to be used in many industrial sectors. A previous study by Yanti et al (2019) obtained three lipolytic filamentous fungi from this genus, namely Aspergillus niger KE1, Aspergillus terreus KC1, and Aspergillus fumigatus KE6, isolated from Kendari landfill soil. These filamentous fungi were the selected lipolytic isolates according to qualitative screening using the rhodamine B agar method.…”