The Potential of Corchorus olitorius Seeds Buccal Films for Treatment of Recurrent Minor Aphthous Ulcerations in Human Volunteers

Abstract

Aphthous ulcers are very common disorders among different age groups and are very noxious and painful. The incidence of aphthous ulcer recurrence is very high and it may even last for a maximum of 6 days and usually, patients cannot stand its pain. This study aims to prepare a buccoadhesive fast dissolving film containing Corchorus olitorius seed extract to treat recurrent minor aphthous ulceration (RMAU) in addition to clinical experiments on human volunteers. An excision wound model was used to assess the in vivo wound healing potential of Corchorus olitorius L. seed extract, with a focus on wound healing molecular targets such as TGF-, TNF-, and IL-1. In addition, metabolomic profiling using HR-LCMS for the crude extract of Corchorus olitorius seeds was explored. Moreover, molecular docking experiments were performed to elucidate the binding confirmation of the isolated compounds with three molecular targets (TNF-α, IL-1β, and GSK3). Additionally, the in vitro antioxidant potential of C. olitorius seed extract using both H2O2 and superoxide radical scavenging activity was examined. Clinical experiments on human volunteers revealed the efficiency of the prepared C. olitorius seeds buccal fast dissolving film (CoBFDF) in relieving pain and wound healing of RMAU. Moreover, the wound healing results revealed that C. olitorius seed extract enhanced wound closure rates (p ≤ 0.001), elevated TGF-β levels and significantly downregulated TNF-α and IL-1β in comparison to the Mebo-treated group. The phenotypical results were supported by biochemical and histopathological findings, while metabolomic profiling using HR-LCMS for the crude extract of Corchorus olitorius seeds yielded a total of 21 compounds belonging to diverse chemical classes. Finally, this study highlights the potential of C. olitorius seed extract in wound repair uncovering the most probable mechanisms of action using in silico analysis.

In Vitro and Randomized Controlled Clinical Study of Natural Constituents’ Anti-HPV Potential for Treatment of Plantar Warts Supported with In Silico Studies and Network Analysis

“…Morphological characterization of prepared film using SEM revealed a smooth background of the film components with small particles well distributed on this base, as seen in Figure 2. The figure shows good distribution of the extract that imparts good loading abilities of the polymer mixture [26]. Morphological characterization of prepared film using SEM revealed a smooth background of the film components with small particles well distributed on this base, as seen in Figure 2.…”

New cytotoxic dammarane type saponins from Ziziphus spina-christi

“…Fraction IV (5 g) was fractionated on a polyamide-6 column (50-160 μm, 1000 × 5 cm, 100 g) using gradient elution starting with water (H 2 O) and ending with MeOH in the order of increasing polarities (0, 5,10,15,20,25,30,35,40,45,50,60,80 and 100%, 1000 mL each, FR 5 mL/min). The effluents from the column were collected in fractions (250 mL each); and each collected fraction was concentrated and monitored by TLC using the system EtOAc: acetic acid: formic acid: H 2 O 10: 1: 1: 2 and detected using PAA reagents.…”

“…A range of secondary metabolites, including as glucosinolates, alkaloids, triterpenoids, flavonoids, saponins, pigments, and tannins, are medicinally important phytochemical substances 7,8 . Numerous investigations on the use of secondary metabolites of plants in conventional medicine have been done [9][10][11] . These secondary metabolites were found to have anti-inflammatory, antimicrobial, antiviral, cardioprotective, and anticancer effects 12,13 .…”

Etiopathogenetic features of recurrent aphthous stomatitis: modern approaches to prevention and treatment

“…Обов'язковий етап -професійна гігієна, усунення травмувальних факторів і санація хронічних вогнищ інфекцій [26]. Рекомендовано використовувати м'яку зубну щітку, зубну пасту, що не містить лаурилсульфат натрію, а також рідину для полоскання рота без спирту [16,24].…”