“…In addition, because of numerous antinutritional factors (ANFs), the use of SBM in aquafeed has undesirable effects on the liver, intestinal villi structure, digestive enzymes and gut microbiota of carnivorous and omnivorous fish species (Aydın & Gümüş, 2020; Gatlin et al, 2007; Mohammadi et al, 2020; Urán et al, 2008; Wang et al, 2017). Pretreatment methods such as dehulling, germination, roasting, soaking, micronization, extrusion, heat, enzymatic and fermentative treatments can reduce ANF presence and improve plant protein digestibility and utilization by farmed fish (Anwar et al, 2020; Hassaan et al, 2015; Krogdahl et al, 2020; Kumar et al, 2020; Xie et al, 2021). Nonetheless, alternative feed ingredients must be identified, and the formulation of fish feed with non‐traditional, plant‐based feed ingredients has gained momentum in recent years to reduce feed costs and address environmental concerns (Ayyat et al, 2021; Faheem et al, 2020).…”