The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering

Rahman, Rozlin Abdul; Sukri, Norhamiza Mohamad; Nazir, Noorhidayah; Radzi, Muhammad Aa'zamuddin Ahmad; Zulkifly, Ahmad Hafiz; Ahmad, Abdul Aziz; Hashi, Abdurezak Abdulahi; Rahman, Suzanah Abdul; Sha'ban, Munirah

doi:10.1016/j.tice.2015.06.001

Cited by 27 publications

(19 citation statements)

References 64 publications

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“…In the repair of cartilage defects, tissue engineering strategies using carrier matrix coupled with cells to regenerate tissue are highly recommended (5). Shaped cartilage has been regenerated in vitro and in immunocompromised animals using chondrocytes and scaffolds (6).…”

Section: Introductionmentioning

confidence: 99%

Protective effects of baicalin on rabbit articular chondrocytes in vitro

Huang

Wang

et al. 2017

Experimental and Therapeutic Medicine

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Drug therapy is one of the typical treatments for post-injury inflammation of cartilage. Traditional Chinese herbs have potential as treatments, as their long history of clinical application has demonstrated they are effective and induce minimal side effects. Baicalin is a traditional Chinese medicine that has been used to treat inflammation, fever, ulcers and cancer for hundreds of years. Previous studies have demonstrated that baicalin may decrease levels of interleukin-1β and suppress the expression of type-I collagen, thus attenuating cartilage degeneration. In the present study, the effect of baicalin on chondrocytes was assessed by examining the morphology, proliferation, extracellular matrix (ECM) synthesis and cartilage-specific gene expression of chondrocytes. The results indicated that baicalin may promote the proliferation of articular chondrocytes, secretion of cartilage ECM and collagen type II, aggrecan and SRY box (Sox) 9 gene upregulation. The expression of collagen I, a marker of chondrocyte dedifferentiation, was downregulated by baicalin; therefore, baicalin may maintain the phenotype of chondrocytes. Within the recommended concentrations of baicalin ranging from 0.625–6.25 µmol/l cell proliferation was increased and a 1.25 µmol/l dose of baicalin exerted the most positive effect on articular chondrocytes. The results of the present study may therefore indicate that baicalin may be used as a novel agent promoting the repair of articular cartilage damage.

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Section: Introductionmentioning

confidence: 99%

Protective effects of baicalin on rabbit articular chondrocytes in vitro

Huang

Wang

et al. 2017

Experimental and Therapeutic Medicine

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“…42 MSCs are generally obtained from the bone marrow and can be studied in vitro , which enables the convenient study of these tissue specific cells. 43 MSCs are being used in in vivo studies because they are governed by the molecules in their environment where their anabolic capacity is modulated.…”

Section: Stem Cells In Bone Regenerationmentioning

confidence: 99%

Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering

2016

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Presently, there is a high paucity of bone grafts in the United States and worldwide. Regenerating bone is of prime concern due to the current demand of bone grafts and the increasing number of diseases causing bone loss. Autogenous bone is the present gold standard of bone regeneration. However, disadvantages like donor site morbidity and its decreased availability limit its use. Even allografts and synthetic grafting materials have their own limitations. As certain specific stem cells can be directed to differentiate into an osteoblastic lineage in the presence of growth factors (GFs), it makes stem cells the ideal agents for bone regeneration. Furthermore, platelet-rich plasma (PRP), which can be easily isolated from whole blood, is often used for bone regeneration, wound healing and bone defect repair. When stem cells are combined with PRP in the presence of GFs, they are able to promote osteogenesis. This review provides in-depth knowledge regarding the use of stem cells and PRP in vitro, in vivo and their application in clinical studies in the future.

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“…Various surgical approaches are widely used to repair injured cartilage, including multiple drilling to encourage revascularization, abrasion arthroplasty, and perichondrial resurfacing. However, the efficacy of such strategies remains controversial, and these approaches are also unsatisfactory in terms of restoring the original structure and function of cartilage [ 6 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Autologous chondrocyte implantation (ACI) was the first strategy employed in clinical practice, utilizing chondrocytes harvested from an area of the patient's own cartilage with diminished weight-bearing function [ 11 – 13 ]. However, several problems were reported with this technique, such as limited proliferative potential of the obtained chondrocytes and loss of functional cell phenotypes in culture [ 6 , 11 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering

Focaroli

Teti

Salvatore

et al. 2016

Stem Cells International

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Articular cartilage is a highly organized tissue with complex biomechanical properties. However, injuries to the cartilage usually lead to numerous health concerns and often culminate in disabling symptoms, due to the poor intrinsic capacity of this tissue for self-healing. Although various approaches are proposed for the regeneration of cartilage, its repair still represents an enormous challenge for orthopedic surgeons. The field of tissue engineering currently offers some of the most promising strategies for cartilage restoration, in which assorted biomaterials and cell-based therapies are combined to develop new therapeutic regimens for tissue replacement. The current study describes the in vitro behavior of human adipose-derived mesenchymal stem cells (hADSCs) encapsulated within calcium/cobalt (Ca/Co) alginate beads. These novel chondrogenesis-promoting scaffolds take advantage of the synergy between the alginate matrix and Co+2 ions, without employing costly growth factors (e.g., transforming growth factor betas (TGF-βs) or bone morphogenetic proteins (BMPs)) to direct hADSC differentiation into cartilage-producing chondrocytes.

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The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering

Cited by 27 publications

References 64 publications

Protective effects of baicalin on rabbit articular chondrocytes in vitro

Protective effects of baicalin on rabbit articular chondrocytes in vitro

Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering

Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering

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