The Potential Effect of Rhizoma coptidis on Polycystic Ovary Syndrome Based on Network Pharmacology and Molecular Docking

Abstract

Background. Rhizoma coptidis (RC) showed a significant effect on PCOS, but its mechanism in PCOS remains unclear. Methods. The components of RC were searched by TCMSP. The Smiles number of the active ingredients was queried through PubChem, and the predicted targets were obtained from the SwissTargetPrediction database. The DrugBank, GeneCards, and DisGeNET databases were retrieved to acquire the related targets of PCOS. Then, the network of compound-target was constructed. The core targets were analyzed using protein-protein interaction (PPI) analysis, and the binding activities were verified by molecular docking. The enriched pathways of key targets were examined by GO and KEGG. Results. 13 components and 250 targets of RC on PCOS were screened. The core network was filtered based on topological parameters, and the key components were palmatine, berberine, berberrubine, quercetin, and epiberberine. The key targets included DRD2, SLC6A4, CDK2, DPP4, ESR1, AKT2, PGR, and AKT1. Molecular docking displayed that the active ingredients of RC had good binding activities with potential targets of PCOS. After enrichment analysis, 30 functional pathways were obtained, including neuroactive ligand-receptor interaction, dopaminergic synapse, and cAMP signaling pathway. Conclusion. In summary, this study clarified the potential effect of RC on PCOS, which is helpful to provide references for clinical practice. It is also conducive to the secondary development of RC and its monomer components.

Assessment of the Effect of Leonurine Hydrochloride in a Mouse Model of PCOS by Gene Expression Profiling

“…Previous studies have shown that calcium acts as a second messenger in most species and all mammals, that calcium signalling can restore oocyte meiosis and activate the egg, which is essential for the regulation of embryonic development [48,49], and that the activation of calcium signalling pathways can inhibit follicular atresia [50]. cAMP signals can regulate the excitability of gonadotropin-releasing hormone (GnRH) neurons [51,52]. GnRH regulates the levels of female reproductive hormones such as luteinising hormone, which affects ovulation.…”

Pemberian Ekstrak Etanol Pelepah Batang Pisang Ketip Dapat Meringankan Polycystic Ovary Syndrome pada Tikus Putih Model

“…Hal ini dapat menyebabkan siklus menstruasi menjadi tidak teratur disertai jumlah folikel kista (Cystic Folicle) yang terbentuk meningkat. Dengan demikian, ovarium yang mengalami PCOS memiliki bobot yang lebih berat jika dibandingkan dengan ovarium normal (Rajan et al, 2017;Hong et al, 2019;Qiu et al, 2019;Shaaban et al, 2021;Duan et al, 2021). Selain perubahan kadar hormon, juga terjadi gangguan toleransi glukosa (impaired glucose tolerance/IGT) pada wanita yang mengalami PCOS secara substansial kadar glukosa lebih tinggi bila dibandingkan dengan wanita tanpa PCOS yang sesuai dengan usia dan bobot badan (Qiu et al, 2019).…”