The Potency of Monoterpenes Contained in Essential Oils of Canary Sap (Canarium indicum L.) as Anti-inflammatory Agent on Asthmatic Rats (Rattus norvegicus)

Abstract

The aim of this study is to determine the potency of EO canary therapy towards level of malondialdehyde (MDA) and lung histopathology of asthmatic rats. The asthmatic rats were prepared by sensitization of allergen conducted by intraperitoneal injection and nebulized of ovalbumin (OVA) also intrasulcular injection of lipopolysaccharide from Phorphyromonas gingivalis bacteria. Five groups of rats (Rattus norvegicus) were used in this research; i.e. the control group, the asthmatic group, and three other groups with therapy of EO canary with the dose of 25, 50, and 100 mg/kg body weight for 7 days. The EO canary contents were analyzed by gas chromatography-mass spectrometry (GC-MS). The MDA levels were measured using the thiobarbituric acid (TBA) reagent and histopathology of bronchial stained using Hematoxilin-Eosin (HE) and observed microscopically. The results showed that EO canary significantly (p < 0.01) decrease MDA levels and repair lung histopathology of asthmatic rats. The analysis of EO canary performed by GC-MS composed of thujene, α-pinene, camphene, sabinene, 2-β-pinene, βmyrcene, l-phellandrene, p-cymene, γ-terpinene, cis-Sabinene hydrate, α-terpinolene, camphor, limonene dioxide, sabinyl acetate, piperitone oxide, ascaridole, α-cubene, αcopaene, trans-caryophyllene, and 1,8-cineol. It can be concluded that EO canary has potency as anti-inflammatory agent of asthmatic condition. The most effective dose therapy was obtained as high as 100 mg/kg body weight that decreased 46.56 % level of MDA.

Linalool: Therapeutic Indication And Their Multifaceted Biomedical Applications

“…The study indicated that essential oil canary has promised anti-inflammatory medication for asthmatic disorders. The most efficacious dose therapy was found to be 100 mg/kg body weight, which resulted in a 46.56 % reduction in malondialdehyde levels [30].…”