The potency of bovine PPD tuberculins in guinea-pigs and in tuberculous cattle

Dobbelaer, R.; O'Reilly, L.M.; Genicot, A; Haagsma, J

doi:10.1016/s0092-1157(83)80008-0

Cited by 14 publications

(11 citation statements)

References 4 publications

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“…Paterson recommends that guinea pigs be used for the control at preparation with occasional check assays in cattle but that if the type of tuberculin is changed or if a change in character is suspected that appeal must be to the assay in cattle [66]. Changes in manufacturing and production procedures may also result in fluctuations in tuberculin potency and there may also be considerable variability in potency between batches of tuberculin, including those produced in the same centre [83, 85, 86]. Tuberculin potency fluctuation was seen during 1990–1992 associated with Good Laboratory Practice adaptations and in 2000 associated with changes instigated as a consequence of EU requirements in relation to Transmissible Spongiform Encephalopathies (unpublished observations—DAFF records).…”

Section: Tuberculin Testsmentioning

confidence: 99%

Perspectives on the History of Bovine TB and the Role of Tuberculin in Bovine TB Eradication

Good¹,

Duignan²

2011

Veterinary Medicine International

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Tuberculosis remains a significant disease of animals and humans worldwide. Bovine tuberculosis is caused by Mycobacteria with an extremely wide host range and serious, although currently probably underdiagnosed, zoonotic potential. Where bovine tuberculosis controls are effective, human zoonotic TB, due to Mycobacterium bovis or M. caprae, is uncommon and clinical cases are infrequent in cattle. Therefore, the control and ultimate eradication of bovine tuberculosis is desirable. Tuberculin tests are the primary screening tool used in bovine eradication. The choice of tuberculin test is dependent on the environment in which it is to be used. Tuberculin potency is critical to test performance, and the accurate determination of potency is therefore particularly important. The design of a control or eradication programme should take into consideration the fundamental scientific knowledge, the epidemiological profile of disease, the experience of other eradication programmes, and the presence, in the same ecosystem, of maintenance hosts, in which infection is self-sustaining and which are capable of transmitting infection. A control or eradication programme will necessarily require modification as it progresses and must be under constant review to identify the optimal desirable goals, the efficacy of policy, and constraints to progress.

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Section: Tuberculin Testsmentioning

confidence: 99%

Perspectives on the History of Bovine TB and the Role of Tuberculin in Bovine TB Eradication

Good¹,

Duignan²

2011

Veterinary Medicine International

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“…In conclusion, the use of A60 as a new tuberculin presents the following advantages, with respect to conventional PPD, for cutaneous testing in tuberculosis: a) a single electrophoretic component in the case of A60 [5] instead of a series of polymers differing in relative concentrations for different PPD preparations [ 13,23]; b) a spectrophotometric determination of A60 [5], which contrasts with the error-prone and subjective titration of PPD by biological methods [14,16,21,29]; and c) an approximately tenfold higher potency, which makes it possible to lower proportionally the amount of injected reagent in cutaneous testing. In addition, the observed instability of PPD preparations, presumably due to the presence of proteolytic enzymes, is likely to be suppressed in the case of A60 preparations, which are quite stable in lyophilized form or in solution at low temperature.…”

Section: Discussionmentioning

confidence: 99%

Delayed hypersensitivity reactions by the mycobacterial antigen A60 and cutaneous testing in tuberculosis

Benoit

Beschin

Desmecht³

et al. 1989

Med Microbiol Immunol

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Antigen A60 has been purified from the cytoplasm of Mycobacterium bovis BCG, and its composition has been determined: it has proved to be able to elicit immune reactions of both humoral and cellular type. Inoculation of A60 into the footpad of mice previously sensitized with the same antigen, or with whole mycobacterial cells produced a footpad swelling showing a peak at 24 h. Similar delayed hypersensitivity reactions were induced in sensitized guinea-pigs by subcutaneous injection of an A60 dose of 0.01 micrograms (minimal revealing dose). A quantity thousandfold higher (15 micrograms A60) was unable to induce in unsensitized guinea pigs the mounting of a cellular immunisation against A60, as shown by negative cutaneous testings 1 month later. Our results show that A60 preparations satisfied the requirements of the European Pharmacopoeia Commission and met the WHO recommendations for new tuberculins. Handicaps of old tuberculin and PPD (heterogeneous mixtures titrated biologically and unstable in solution) can be overcome by A60 preparations (a single antigen spectrophoretically measurable and stable).

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“…Compulsory cutaneous testing for bovine tuberculosis is carried out by intradermal injection of the bovine tuberculin purified protein derivative (bovine tuberculin-PPD), which is a steamsterilized culture filtrate extract of Mycobacterium boris. Likewise, the same type of extracts from Mycobacterium paratuberculosis (johnin-PPD) or Mycobacterium avium (avian tuberculin-PPD) are used as reagents in cutaneous testing for paratuberculosis (Johne's disease) [1,2]. Because of the genetic and immunological kinship of M. paratuberculosis and M. avium, the former organism is frequently replaced by the latter (which is more easy to cultivate), in the preparation of sensitins (tuberculin-like preparations) used for the diagnosis of paratuberculosis [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of three sensitins used in cutaneous testing for tuberculosis and paratuberculosis in cattle

Gilot¹,

Cocito²

1993

FEMS Microbiology Letters

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Correspondence between components of sensitins (avian, bovine, and johnin‐PPPD tuberculins) and the proteins of the corresponding microorganisms (Mycobacterium avium, Mycobacterium paratuberculosis) was established. Most of these identified proteins are present in the three organisms, but the 38.3‐ and 48.8‐kDa proteins of M. avium and the 54.0‐ and 58.7‐kDa proteins of M. bovis are endowed with species‐specific epitope(s).

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The potency of bovine PPD tuberculins in guinea-pigs and in tuberculous cattle

Cited by 14 publications

References 4 publications

Perspectives on the History of Bovine TB and the Role of Tuberculin in Bovine TB Eradication

Perspectives on the History of Bovine TB and the Role of Tuberculin in Bovine TB Eradication

Delayed hypersensitivity reactions by the mycobacterial antigen A60 and cutaneous testing in tuberculosis

Comparative analysis of three sensitins used in cutaneous testing for tuberculosis and paratuberculosis in cattle

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