The possible therapeutic effect of ‘Chaetomium globosum’ fungal extract on experimentally induced rheumatoid arthritis

Abstract

BackgroundRheumatoid arthritis (RA) is an autoimmune disease that leads to chronic inflammation in the joints with subsequent cartilage and bone destruction. RA induces a massive burden on health services worldwide. Aim of the work This study was designed to evaluate the possible therapeutic effect of 'Chaetomium globosum' extract in the treatment of RA in a rat model. Materials and methodsForty male Wistar albino rats aged 8-10 weeks were divided into four groups of 10 rats each. The control group (group I) was injected subcutaneously with 0.1 ml of saline. RA was induced in the other three groups (groups II, III, and IV) by single subcutaneous injection of 0.1 ml of complete Freund's adjuvant in the footpad of the right hind paw. Group II was induced with arthritis and left untreated. Rats in groups III and IV were treated by administering either C. globosum 10 µg/kg or methotrexate (MTX) 0.3 mg/kg subcutaneously twice weekly for 2 weeks from day 12 after induction of arthritis. Animals of all groups were sacrificed on day 28 from the start of the experiment. The ankle joints were processed and stained with H&E, Masson's trichrome, and immunohistochemical stain for inducible nitric oxide synthase. Specimens were also processed for transmission electron microscopic study. ResultsUntreated arthritic rats revealed paw swelling, synovial hyperplasia, inflammatory infiltration, collagen accumulation, cartilage degradation, bone destruction, and significant increase in inducible nitric oxide synthase-positive chondrocytes. Transmission electron microscopic examination confirmed these results. The present study demonstrated for the first time that C. globosum significantly reduced all the clinical and histopathological changes of arthritis similar to MTX. Conclusion C. globosum extract had therapeutic effects similar to the well-established drug MTX and could be devoid of its serious side effects.

Histological study on freund's complete adjuvant induced arthritis in rat models following treatment with crude Egyptian scorpion venom and methotrexate

“…The observed histopathological changes of proximal interphalangeal joints of the experimental groups reveals that, disease control group of arthritic rats joint showed prominent abnormalities from the normal joint like edema formation, degeneration with partial erosion of the cartilage, destruction of bone marrow and extensive infiltration of inflammatory exudates in the articular surface. These observations were in agree to those were demonstrated by Bauerova, Paulovicova [10] and Xie, Ma [11] and Makhlouf, Khalil [12] . The standard treated group joint showed normal bone marrow with less cellular infiltrates.…”

Evaluation of the possible therapeutic role of omega-3 on ankle joint and lung in a model of rheumatoid arthritis in rats: A histological and immunohistochemical study

“…Omega-3 fatty acids are a group of poly-unsaturated fatty acids with multiple double bonds. It is mainly gained from seafood consumption [10,11] . Omega-3 fatty acids are essential fatty acids that cannot be manufactured by the body, consequently, they must be obtained from food or taken as supplements.…”

“…They are found mainly in fish oils (salmon and tuna fish), canola oil and walnuts [12] . They have long been known to possess a wide range of beneficial effects, including the treatment of various forms of chronic inflammatory diseases [11] .…”

Bioactive Properties and Metabolite Profiles of Endolichenic Fungi in Mangrove Ecosystem of Negombo Lagoon, Sri Lanka

“…The IC 50 values shown by Neofusicoccum parvum , C. globosum and Curvularia lunata were 49.09 ± 0.82, 99.53 ± 1.34, and 162.7 ± 2.61 µg/mL, respectively. The available records show their potency against chronic inflammation, 18–20 but interestingly, none of them were reported from ELF.…”