The possible protective role of quercetin on induced cardiac Oxidative DNA Damage by repeated exposure to diesel exhaust nanoparticles in rats (a histological and immunohistochemical study)

Abstract

Diesel exhaust nanoparticles (DENPs) are gaseous exhaust and one component of particulate matter (PM) air pollution which is found to cause health hazards in humans. Quercetin is widely known for its antioxidant and antitoxic potential. We aimed todetect the protective effect of quercetin in DENP-induced cardio toxicity. Fifty Malealbino rats were divided into two groups,first group (ten rats) divided into two equal subgroups (one receive saline act as control and other receive quercetin,the second group (forty rats)divided equally into two subgroups (one was treated with repeated doses of DENPs [90µg/ rat and 180µg/ rat for 6 days intratracheally every two days] and other treated orally with quercetin (60 mg/kg B.Wt.) 1h prior to DENP exposure,DNA damagewas examined by measureserum8-hydroxydeoguanosine (8-OHdG) levels followed by histological and immunohistochemical studies for nuclear factor-kappa B(NF-κB) expression in heart tissues. Tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6) and serum level 8-Ohd Gare significantly higher in repeated doses of DENPs than the control group. Pretreatment with quercetin reduced significantly serum level 8-OhdGwhen compared to DENPs group.Cardiac myocytes disruption, vacuolation, inflammation and wide separation of its fibers in contrast to control group. Myocyteinflammations were significantly decreased with quercetin group compared with control group (decrease NF-κBimmunostaining). The present work yields experimentalevidence that quercetin can reduce oxidative DNA damage and inflammation induced by DENPs, so suggested that quercetin possible cardioprotective effect against DENPs.

Effects of Mesenchymal Stem Cells and Their Derived Microvesicles on Pulmonary Toxicity Induced by Petrol Exhaust Nanoparticle; Histological and Immuno-Histochemical Study

“…MSC-MVs have many therapeutic effects in different diseases, as renal injury, brain injury, heart injury and lung injury [34]. The therapeutic ability of MSC-MVs has been studied in different disease models, showing a similar or even superior effect to MSCs themselves [35][36] which has showed in our result.…”

Impact of quercetin on pulmonary toxicity induced by diesel exhaust nanoparticles in albino rat : Histological, immunohistochemical and biochemical study

“…Quercetin has significant anti-inflammatory effects and antioxidant properties with subsequent amelioration of DENPs-induced damage in different tissues [8,10] . Our findings agreed with results reported by Huang et al [9] who stated that quercetin attenuated lipopolysaccharideinduced pathological changes in the lung tissue where the inflammatory cell infiltration and alveolar wall thickening were attenuated by quercetin pretreatment.…”

“…Quercetin was dissolved in olive oil at a concentration of 60 mg/kg B.Wt and was delivered to rats by oral gavage 1h before instillation of DENPs [10].Quercetin was found to be well tolerated in humans and animals in the given doses [15] .…”

“…Quercetin is a polyphenol (flavonol: (3,3′,4′,5,7 pentahydroxy-flavone) present in vegetables, nuts and fruit [8,9] . Quercetin has several protective and therapeutic effects on human diseases due to its anti-inflammatory, antioxidative, antimicrobial, antiviral, and antiulcerogenic effects [10,11] . Other studies reported that quercetin has also hypolipidemic, antiplatelet properties, antihypertensive, antineoplastic and antihepatotoxic [12] .Quercetin inhibits cyclooxygenase and lipoxygenase pathways at high doses, whereas at lower doses the lipoxygenase pathway is the main target of quercetin anti-inflammatory activities [13] .…”