The possible protective role of 6-gingerol on a model of hepatic injury induced by carbon tetrachloride in adult male albino rats: Histological and immunohistochemical study

Abstract

Introduction: Carbon tetrachloride (CCL 4) is a potent environmental hepatotoxin that was commonly used in industrial applications. The main active component in ginger is 6-gingerol which is responsible for many of its medicinal properties. Aim of the work: To assess the possible protective role of orally administered 6-gingerol on a model of hepatic injury induced by CCL 4 in adult male albino rats. Materials and Methods: Thirty adult male albino rats were used in this study and were divided into three groups. Group I: control group which was further subdivided into three subgroups, group II: received intraperitoneal injection of CCL 4 twice / week for two weeks. Group III: received oral 6-gingerol daily five days before the injection of CCL 4-as in group II-and continued till the end of experiment. After two weeks from last injection of CCL 4. All rats were sacrificed; the right lobe of the liver was taken and processed. Sections stained by H&E, Mallory`s trichrome stain and immunostaining for TNF-α and PCNA were prepared. Morphometric and statistical analysis were also performed. Results: Liver of group II showed hepatocytes with vacuolated cytoplasm and congested hepatic sinusoids. Congested portal veins and dilated bile ducts were also seen at portal tract areas. Increased collagen fibers at the portal tract areas were seen by Mallory's trichrome. Increased intensity of positive immune reaction for TNF-α and PCNA was also observed in this group. In group III the liver showed picture nearly similar to that of the control. Morphometric and statistical analysis confirmed the histological and immunohistochemical results. Conclusions: The present work concluded that 6-gingerol could provide a good protection of the liver in a model of hepatic injury induced by CCL 4 .

The Natural Antioxidants Curcumin and Silymarin Afforded Hepatoprotective Effects against Deferasirox-Induced Hepatotoxicity

“…In the present work, H&E-stained liver sections of Deferasirox group revealed disturbed hepatic lobular architecture, swollen hepatocytes with highly vacuolated cytoplasm and shrunken deeply stained pyknotic nuclei, extensive in ammatory cellular in ltration and proliferation of bile ducts. Similar results were obtained by other authors [39,40] studying the damaging effect of zinc oxide nanoparticles and carbon tetrachloride on rat's liver, respectively.…”

Promising effects of gingerol against toxins: A review article

“…Moreover, 6-gingerol modulated TNF-α and proliferating cell nuclear antigen (PCNA) expression increased in rats treated with CCl 4. 242 Therefore, gingerols have therapeutic and protective properties on the CCl 4 -induced liver and DNA toxicity. 75,241,242 Phorbol Phorbol esters with tigliane skeletal structure belong to the diterpenes group that are amphiphilic molecules.…”

“…242 Therefore, gingerols have therapeutic and protective properties on the CCl 4 -induced liver and DNA toxicity. 75,241,242 Phorbol Phorbol esters with tigliane skeletal structure belong to the diterpenes group that are amphiphilic molecules. 243 The amphiphilic molecules bind to receptors of the phospholipid membrane and trigger the signal transduction pathways.…”

Evaluation of the impact of platelet rich plasma and hyaluronic acid on ketamine-induced cystitis in albino rats : A histological and immunohistochemical study.

“…Proliferating cell nuclear antigen (PCNA) is a nonhistone nuclear protein that used as a standard marker for cellular proliferation activity [36] . Our findings were coincided with previous researches [12,23,37] , who stated that urothelium is a very stable type of tissue and has a slow proliferative activity.…”