The possibilities of potentiating the efficacy of probiotics

Bomba, Alojz; Nemcová, Radomí­ra; Mudroňová, Dagmar; Guba, P.

doi:10.1016/s0924-2244(02)00129-2

Cited by 101 publications

(52 citation statements)

References 25 publications

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“…The following experiment was designed in order to evaluate the addition of salts (KH 2 The count of Bifidobacterium cells was carried out after 30 days of refrigerated storage of yoghurt at 7ºC in RCPB pH5 agar fortified with salts and infusion of dehydrated liver and in RCPB pH5 fortified with only the dehydrated liver infusion (RCPB pH5-Liver).…”

Section: Effect Of Adding Salts In the Fortification Of Rcpb Ph5 Agarmentioning

confidence: 99%

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Evaluation of culture media for counts of Bifidobacterium animalis subsp. lactis Bb 12in yoghurt after refrigerated storage

Fachin¹,

Moryia²,

Gândara³

et al. 2008

Braz. J. Microbiol.

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The agar RCPB pH5 has been considered a good alternative for counts of Bifidobacterium in yoghurt. However, during the refrigerated storage of yoghurt it is extremely difficult to count this microorganism due to the size of the colonies, which are so small they require the aid of a stereoscope to count them. Another agar, MRS-LP, has been also recommended for counts of Bifidobacterium in the presence of yoghurt bacteria. This study evaluated the supplementation of RCPB pH5 agar with dehydrated liver extract and the salts KH2PO4, K2HPO4, FeSO47H2O, MnSO4H2O and MgSO47H2O, aiming at improving the differentiation of Bifidobacterium in yoghurt after refrigerated storage, and also evaluated the selective count of Bifidobacterium in yoghurt using the agar MRS-LP. The agar MRS-LP presented the same cell recovery as non-fortified RCPB pH5 agar, used as a standard medium, thus being considered a good option for counts of Bifidobacterium in yoghurt. The fortified RCPB pH5 also presented the same recovery as the standard RCPB pH5 medium, however, the addition of dehydrated liver extract to the RCPB pH5 agar considerably increased the size of the Bifidobacterium colonies after refrigerated storage, making differentiation of the colonies much easier and reliable when compared to the standard non-fortified RPCP pH5. The addition of the salts (KH2PO4, K2HPO4, FeSO47H2O, MnSO4H2O and MgSO47H2O) had no influence on the performance of the RCPB pH5 agar.

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Section: Effect Of Adding Salts In the Fortification Of Rcpb Ph5 Agarmentioning

confidence: 99%

“…Currently much attention is given to the use of probiotic yoghurts due to their potential benefits to human health (2). It is recommended that each product should indicate the minimum daily amount required for it to confer health benefit(s) based on in vitro (animal) and human studies (3).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of culture media for counts of Bifidobacterium animalis subsp. lactis Bb 12in yoghurt after refrigerated storage

Fachin¹,

Moryia²,

Gândara³

et al. 2008

Braz. J. Microbiol.

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“…However, probiotic action is non-specific and non-discriminatory or ineffective in certain hosts. 45 This is in part due to broad mode of action and strain variability ( Table 2). Probiotics differ from one another, therefore, the beneficial attributes of one strain or a cocktail of strains may not be reproducible and may vary from person to person.…”

Section: Recombinant Bioengineered Probioticsmentioning

confidence: 99%

Bioengineered probiotics, a strategic approach to control enteric infections

Amalaradjou

Bhunia

2013

Bioengineered

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“…Res., 9 (5): 223-236, 2015Al-Sunaiher et al, 2010Spanggaard et al, 2000). As a promoter of health, probiotics could be beneficial through multiple ways, either by a single strain introduction or as a combination of several probionts, which include the inhibiting pathogens through production of antagonistic compounds, competition for adhesion sites and nutrients, alteration of enzymatic activities of pathogens, immunostimulatory functions, improving feed digestibility, promotion of growth and survival and also improvement to water quality (Utiswannakul et al, 2011;Nimrat et al, 2008;Balcazar et al, 2006;Bomba et al, 2002). As proposed by Merrifield et al (2010), probiotics must not be pathogenic and should display antagonistic properties against one or multiple pathogens.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the Probiotic Potential of Malaysian Paenibacillus pabuli Against Vibrio alginolyticus in Artemia Culture

Ina-Salwan¹,

Hishammudd²,

Zulperi³

et al. 2015

Asian J. of Agricultural Research

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In the present study, two strains of Malaysian Paenibacillus spp. isolated from intestinal tract of cultured hybrid red tilapia were tested for their antagonistic activity against Vibrio alginolyticus, a destructive bacterial pathogen to marine fishes as probiotics in aquaculture. Homology searches of 16S rRNA and Internal Transcribes Spacer (ITS) genes sequencing with nucleotide Basic Local Alignment Sequence Tool (nBLAST) revealed that both strains were identical to Paenibacillus spp. and Paenibacillus pabuli, respectively. In vitro and in vivo experiments were conducted to study the efficacy of these Paenibacillus strains as probiotics. Co-culture assay demonstrated that the growth of V. alginolyticus was inhibited when grown with these probionts. A complete inhibition of the pathogen by P. pabuli strain D12 occurred at 48 h of incubation period. To qualify strains as probiotics, Artemia challenged with V. alginolyticus was performed, with or without these potential probiotics. The highest survival rate (72%) of Artemia was observed in P. pabuli strain D12 treatment after challenged with V. alginolyticus, followed by P. pabuli strain D14 at 68% survival rate. The lowest survival of Artemia (23% survival rate) was recorded when challenged with only V. alginolyticus. This study reported the ability of Paenibacillus D12 and D14 as potential probiotics to control V. alginolyticus in Artemia culture system.

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The possibilities of potentiating the efficacy of probiotics

Cited by 101 publications

References 25 publications

Evaluation of culture media for counts of Bifidobacterium animalis subsp. lactis Bb 12in yoghurt after refrigerated storage

Evaluation of culture media for counts of Bifidobacterium animalis subsp. lactis Bb 12in yoghurt after refrigerated storage

Bioengineered probiotics, a strategic approach to control enteric infections

Elucidating the Probiotic Potential of Malaysian Paenibacillus pabuli Against Vibrio alginolyticus in Artemia Culture

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