Bioengineered probiotics, a strategic approach to control enteric infections

Amalaradjou, Mary Anne Roshni; Bhunia, Arun K.

doi:10.4161/bioe.23574

Cited by 53 publications

(37 citation statements)

References 100 publications

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“…Recently, bio engineered probiotics are also being studied for their efficacy to prevent rotaviral and other enteric diseases. Similar studies are lacking for avain RVs and thus, needed to know their efficiency against AvRV disease (Amalaradjou & Bhunia, 2015). Cinnamon and its oil have been documented to be effective against RV in man that needs attention against AvRVs (Ranasinghe et al 2013).…”

Section: Herbs and Essential Oils As Antiviral Agentsmentioning

confidence: 99%

Avian rotavirus enteritis – an updated review

Dhama

Saminathan

Karthik

et al. 2015

Veterinary Quarterly

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Section: Herbs and Essential Oils As Antiviral Agentsmentioning

confidence: 99%

Avian rotavirus enteritis – an updated review

Dhama

Saminathan

Karthik

et al. 2015

Veterinary Quarterly

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“…Seventy percent of the enteric infections are attributable to food contamination. 1 Among commonly found bacterial species in food items, spore forming bacteria are notorious due to their ability to produce spores and survive in the typical heat and other treatments used to inhibit undesirable bacterial growth. Once the favorable conditions return, the treatments ramify in proliferation of vegetative cells of spore-formers in absence of competition from other non-spore forming bacteria.…”

Section: Introductionmentioning

confidence: 99%

Synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications

Ghosh¹,

Patil²,

Sharma³

et al. 2013

IJN

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Silver has long been advocated as an effective antimicrobial. However, toxicity issues with silver have led to limited use of silver in nanoform, especially for food preservation. With the aim of exploring combinatorial options that could increase the antibacterial potency of silver nanoparticles and reduce the effective dosage of silver, we evaluated the extent of synergy that a combination of silver nanoparticles and an essential oil representative (cinnamaldehyde) could offer. A battery of gram-positive and gram-negative bacterial strains was utilized for antibacterial assays, and extents of synergism were calculated from fractional inhibitory concentration indices. The activity of nanoparticles was greatly enhanced when utilized in the presence of cinnamaldehyde. We observed combinatorial effects that were strongly additive against all the bacterial strains tested, and genuine synergy was found against spore forming Bacillus cereus and Clostridium perfringens – bacterial strains associated with release of cytotoxins in contaminated food and known for their persistence. Bacterial kill curve analysis revealed a very fast bactericidal action when a combination of two agents was used. The electron and atomic force microscopy also revealed extensive damage to the bacterial cell envelop in the presence of both agents. We also performed hemolysis assays to investigate and approximate the extent of toxicity exhibited by the two agents, and observed no adverse effect at the concentrations required for synergy. This study shows that safe levels of silver in nanoform in combination with essential oil component cinnamaldehyde can be effectively used for controlling the spore-forming bacterial species.

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“…In order to potentialize the beneficial effects of probiotic strains, research has been conducted over the last decades, based on genetic engineering techniques, especially those related to DNA manipulation. Thus, modern methods of genetic engineering open the new opportunities to design and create genetically modified probiotic strains with the desired characteristics or to exclusively target a specific pathogen or toxin to be used either as a vaccine or for drug delivery [119,120]. Since most of the probiotic strains are part of the LAB group, most of the genetic manipulation studies are carried out with species that belong to this group, such as Lactococcus and Lactobacillus genera.…”

Section: Recombinant Lab Probioticsmentioning

confidence: 99%

Recombinant Probiotics and Microbiota Modulation as a Good Therapy for Diseases Related to the GIT

Jesus¹,

Lima²,

Coelho-Rocha³

et al. 2020

The Health Benefits of Foods - Current Knowledge and Further Development

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Many diseases that affect the gastrointestinal tract (GIT) have great influence on the quality of life of the majority of patients. Many probiotic strains are being highly studied as a promising candidate due to their beneficial effect reported in the GIT. With the purpose of increasing the beneficial characteristics of some probiotics strains and, consequently, to improve further the reported results, many probiotic strains expressing or encoding different proteins, with anti-inflammatory activities, have been developed. These recombinant strains have been reported as good candidates for the treatment of different pathological conditions, especially colitis and mucositis disease since they have been shown to have positive results and good perspectives for GIT inflammation. Thus, this chapter will first address the aspects of the gastrointestinal tract in humans as well as its microbiota. In a second moment, it will discuss about chronic diseases, mainly the intestinal ones. Finally, it will discuss about probiotics, especially concerning on lactic acid bacteria (LAB), and its action in the prevention and treatment of these diseases. At the final part, we will point out aspects on the development of recombinant strains and the results found in the literature on disease models.

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Bioengineered probiotics, a strategic approach to control enteric infections

Cited by 53 publications

References 100 publications

Avian rotavirus enteritis – an updated review

Avian rotavirus enteritis – an updated review

Synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications

Recombinant Probiotics and Microbiota Modulation as a Good Therapy for Diseases Related to the GIT

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