The Polyubiquitin Gene Is Essential for Meiosis in Fission Yeast

Okazaki, Keishi; Okayama, Hiroto; Niwa, Osami

doi:10.1006/excr.1999.4728

Cited by 26 publications

(29 citation statements)

References 38 publications

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“…Ubc-null mice die in mid-gestation due to disruption of fetal liver function (41,42), while mice that are homozygous null for Ubb are viable but sterile (43). Polyubiquitin genes have been found to be required for meiosis in yeast, fly, and mammals (44)(45)(46), suggesting that levels of ubiquitin play specific developmental roles. How this relates to the depletion of ubiquitin by silencing of UBB during tumorigenesis remains unclear.…”

Section: Resultsmentioning

confidence: 99%

Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C

Kedves¹,

Gleim²,

Liang³

et al. 2017

Journal of Clinical Investigation

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Section: Resultsmentioning

confidence: 99%

Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C

Kedves¹,

Gleim²,

Liang³

et al. 2017

Journal of Clinical Investigation

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“…The fission yeast polyubiquitin ubi4 + and budding yeast polyubiquitin Ubi4 are both highly expressed in meiotic cells (Okazaki et al, 2000;Treger et al, 1988), although the level of free Ub was not raised in meiotic cells. Loss of function of polyubiquitin caused defects specifically in meiosis (fission yeast) or sporulation (budding yeast) (Okazaki et al, 2000;Treger et al, 1988). As with magn mutant testes in Drosophila, the level of free Ub was greatly reduced in cells entering meiosis in the yeast ubi4 mutant.…”

Section: Discussionmentioning

confidence: 97%

The polyubiquitin gene Ubi-p63E is essential for male meiotic cell cycle progression and germ cell differentiation in Drosophila

Kim

Fuller

2013

Development

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SUMMARYThe ubiquitin proteasome system (UPS) regulates many biological pathways by post-translationally ubiquitylating proteins for degradation. Although maintaining a dynamic balance between free ubiquitin and ubiquitylated proteins is key to UPS function, the mechanisms that regulate ubiquitin homeostasis in different tissues through development are not clear. Here we show, via analysis of the magellan (magn) complementation group, that loss of function of the Drosophila polyubiquitin Ubi-p63E results specifically in meiotic arrest sterility in males. Ubi-p63E contributes predominantly to maintaining the free ubiquitin pool in testes. The function of Ubi-p63E is required cell-autonomously for proper meiotic chromatin condensation, cell cycle progression and spermatid differentiation. magn mutant germ cells develop normally to the spermatocyte stage but arrest at the G2/M transition of meiosis I, with lack of protein expression of the key meiotic cell cycle regulators Boule and Cyclin B. Loss of Ubi-p63E function did not strongly affect the spermatocyte transcription program regulated by the testis TBP-associated factor (tTAF) or meiosis arrest complex (tMAC) genes. Knocking down proteasome function specifically in spermatocytes caused a different meiotic arrest phenotype, suggesting that the magn phenotype might not result from general defects in protein degradation. Our results suggest a conserved role of polyubiquitin genes in male meiosis and a potential mechanism leading to meiosis I maturation arrest.

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“…In fission yeast, several genes encoding ubiquitin were identified (24). The ubi1 ϩ (SPAC11G7.04) and ubi3 ϩ (SPAC6G10.11c) genes, encoding an N-terminal ubiquitin fused to the ribosomal proteins L40 and S37, respectively, belong to the class of ubiquitin genes whose translation product is a ubiquitin-ribosome fusion protein.…”

mentioning

confidence: 99%

“…AF095794) gene, encoding a multiubiquitin linear repeat of eight ubiquitins linked in tandem, belongs to another class of gene whose translation products are multiubiquitin fusion proteins. The products of ubiquitin genes are posttranslationally processed to ubiquitin monomers (24).…”

mentioning

confidence: 99%

Lub1 Participates in Ubiquitin Homeostasis and Stress Response via Maintenance of Cellular Ubiquitin Contents in Fission Yeast

Ogiso

Sugiura

Kamo

et al. 2004

Molecular and Cellular Biology

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Ubiquitin-dependent proteolysis plays a pivotal role in stress responses. To investigate the mechanisms of these cellular processes, we have been studying Schizosaccharomyces pombe mutants that have altered sensitivities to various stress conditions. Here, we showed that Lub1, a homologue of Ufd3p/Zzz4p/Doa1p in budding yeast, is involved in the regulation of ubiquitin contents. Disruption of the lub1 ؉ gene resulted in monoubiquitin as well as multiubiquitin depletion without change in mRNA level and in hypersensitivity to various stress conditions. Consistently, overexpression of genes encoding ubiquitin suppressed the defects associated with lub1 mutation, indicating that the phenotypes of the lub1 mutants under stress conditions were due to cellular ubiquitin shortage at the posttranscriptional level. In addition, the lub1-deleted cells showed aberrant functions in ubiquitin/proteasome-dependent proteolysis, with accelerated degradation of ubiquitin. Also Cdc48, a stress-induced chaperon-like essential ATPase, was found to interact with Lub1, and this association might contribute to the stabilization of Lub1. Our results indicated that Lub1 is responsible for ubiquitin homeostasis at the protein level through a negative regulation of ubiquitin degradation.

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The Polyubiquitin Gene Is Essential for Meiosis in Fission Yeast

Cited by 26 publications

References 38 publications

Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C

Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C

The polyubiquitin gene Ubi-p63E is essential for male meiotic cell cycle progression and germ cell differentiation in Drosophila

Lub1 Participates in Ubiquitin Homeostasis and Stress Response via Maintenance of Cellular Ubiquitin Contents in Fission Yeast

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