The polymorphisms of bovine cocaine-and amphetamine-regulated transcripts and their associations with cattle (Bos taurus) growth traits

Abstract

We investigated the polymorphisms of bovine cocaine- and amphetamine-regulated transcripts (CART ). The coding and regulating regions of CART were screened in 7 cattle breeds by the single-stranded conformation polymorphism (SSCP) technique. The four loci (C1,C2, C3 and C4) studied were all polymorphic. Polymerase chain reaction (PCR) products representing different SSCP variants were sequenced and a total of 9 single-nucleotide polymorphisms (SNPs) were found. The associations between polymorphic loci and the growth traits of Nanyang cattle were analysed. The results indicated that genotype A1A1 of the C1 locus was associated with a higher body weight (P less than 0.05) than heterozygous A1B1. Genotype A2A2 of the C2 locus was associated with lower body weight and average daily weight gain (P < or = 0.001) than heterozygous A2B2. C3 and C4 loci had no significant effect on Nanyang cattle growth traits (P > 0.05).

Polymorphisms of the cocaine-amphetamine-regulated transcript (CART) gene and their association with reproductive traits in Chinese goats

“…In humans, the missense mutation Leu 34 Phe may lead to obesity (Yanik et al, 2006). Reports in cattle showed that some single nucleotide polymorphisms (SNPs) (-636T→C, -521T→C, -1431T→C, -398T→C, and 234A→G) were associated with growth traits (Zhang et al, 2008). Findings in pigs demonstrated that swine with (CA) 2 (CG) 1 (CA) 9 or (CA) 2 (CG) 3 (CA) 11 had significant effects on meat content carcass (P < 0.05) in both Polish Large White and Polish Landrace breeds (Stachowiak et al, 2009).…”

Correlation between the cocaine- and amphetamine-regulated transcript in the pyloric section of the abomasum and fat deposition in bulls’ carcasses

“…Some of them refer to humans (Kasacka and Piotrowska 2012;Banke et al 2013). There are few sources available dealing with the CART activity in cattle, and the majority of articles mostly discuss CART location in the stomach and the nervous system (Kobayashi et al 2006;Zhang et al 2008;Arciszewski et al 2009;Smithet al 2010;Janiuk et al 2013). Until now, no studies have been performed to analyse the relationship between CART and functional traits of the organism.…”

Detection of Cocaine and Amphetamine Regulated Transcript in the abomasum of slaughtered bulls with different daily body weight gains

“…The current knowledge of CART incidence in ruminants is incomplete, since it only refers to sheep and cattle (Zhang et al 2008;Arciszewski et al 2009). In the case of bovine studies performed inthe last years are fragmentary and concern the expression of this neuropeptide in the centers nerves system in different cattle breeds (Zhang et al 2008).…”

“…In the case of bovine studies performed inthe last years are fragmentary and concern the expression of this neuropeptide in the centers nerves system in different cattle breeds (Zhang et al 2008). The results of these authors have shown a relationship between the amount of secreted CART and the daily body weight gains of the animals.…”

“…The data for cattle are incomplete due to considerable difficulty in obtaining experimental material. The existing papers discuss CART location in the nervous system or CART expression in various cattle breeds (Zhang et al 2008). Despite the available knowledge on the CART peptide, precise answers to the question of its role in particular organs, including the stomach, are missing.…”