The polymorphism of HLA‐A, ‐C, ‐B, ‐DRB3/4/5, ‐DRB1, ‐DQB1 loci in Zhejiang Han population, China using NGS technology

Abstract

The distributions of HLA allele and haplotype are various in the populations. Currently, the data for HLA alleles and haplotypes at three fields resolution level in Chinese Han population is rare. Here, the HLA alleles and haplotypes of the 1734 cord blood samples from Zhejiang Han population, China were reported at three fields resolution. All samples were randomly collected from the Zhejiang Cord Blood Bank, China. HLA-A, -B, -C, -DRB1, -DQB1, -DRB3/4/5 loci was genotyped using next generation sequencing method. The genotypes of the samples were assigned using the HLA TypeStream Visual Software version 1.2.0. The frequency of alleles, haplotype estimation and linkage disequilibrium analysis were performed with the Arlequin software 3.5.2.2. It was found that the top three frequent alleles of HLA-

Simultaneous genotyping for human platelet antigen systems and HLA-A and HLA-B loci by targeted next-generation sequencing

“…The distribution of HLA-A , HLA-B , and HPA systems was similar to those of our previous reports ( 11 , 35 ). Furthermore, some SNVs were detected in the coding region, which is located in the structural domain of the platelet membrane glycoproteins ( Table 3 ).…”

“…At present, many DNA-based methods have been used for HLA and/or HPA genotyping in platelet donors ( 20 – 28 , 30 , 31 ). It has been reported that PCR-SBT and NGS methods have been routinely used for HLA genotyping in the laboratory ( 24 – 26 , 35 , 36 ). The NGS methods for HLA genotyping are divided into targeted and amplicon-based methods ( 34 , 35 ).…”

“…It has been reported that PCR-SBT and NGS methods have been routinely used for HLA genotyping in the laboratory ( 24 – 26 , 35 , 36 ). The NGS methods for HLA genotyping are divided into targeted and amplicon-based methods ( 34 , 35 ). Classical HLA-A , HLA-B , and HLA-C molecules were all expressed in the platelet, but the HLA-C molecule was expressed at a low level.…”

“…The HLA-A and HLA-B loci were also genotyped using an AllType NGS 9-Loci Amplification Kit (One Lambda Inc.) according to the manufacturer’s instructions. The HLA-A and HLA-B genotypes were assigned using the TypeStream Visual Software version 2.0 with the IMGT/HLA Database version 3.46.0.0 (One Lambda Inc.) as previously reported ( 35 ).…”

Identification of the novel HLA‐B*46:95N allele in a Chinese blood donor

“…The HLA‐A, ‐C, ‐B, ‐DRB1 and ‐DQB1 loci of the donor were genotyped using the AllType™ NGS 9‐loci amplification kit (One Lambda Inc., Canoga Park, CA, USA) and the genotype of the donor was assigned using a TypeStream Visual Software version 1.2.0(One Lambda Inc.) as per our previous reports 2–4 . According to the sequence result of NGS, a novel HLA‐B allele, now officially named as HLA‐B*46:95N was identified.…”