The platelet storage lesion

Seghatchian, Jerard; Krailadsiri, Pranee

doi:10.1053/tm.1997.0110130

Cited by 292 publications

(150 citation statements)

References 57 publications

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“…Platelet storage was associated with previously described changes that characterize the platelet storage lesion such as drop of pH and pCO 2 and increase in pO 2 lactate, LDH and soluble P-selectin levels. 3 Flow cytometry analyses also indicated a storage-related increase in CD62P (GMP-140 or P-selectin) expression while CD42b…”

Section: Caspases Are Only Slightly Activated During Platelet Storagementioning

confidence: 99%

“…Altered metabolic activity, as indicated by pH changes, increased lactate production and decreased glucose consumption, as well as lactate dehydrogenase (LDH) release, also reflect the conditions of storage. 3 Many of the other changes which take place in platelets during their storage are similar to the activation of fresh platelets, although they occur much more slowly (several days compared to a few seconds) and are partially reversible. Both activated and stored platelets undergo ␣-granule release, as demonstrated by expression of CD62P (also termed Pselection) on the plasma membrane and release of cleaved CD62P in the plasma.…”

Section: Introductionmentioning

confidence: 99%

“…These results were in accordance with previously published analyses of platelets stored in standard conditions. 3,29 At various times after the beginning of storage, we prepared whole cell lysates as well as lysates from the cytosolic and particulate platelet fractions. The 116-kDa nuclear enzyme, PARP, that can be identified in U937 leukemic cells, was not detected in platelet lysates, suggesting the lack of contamination of platelet concentrates by nucleated cells (Figure 2a).…”

Section: Figurementioning

confidence: 99%

“…2 Platelet storage duration is limited by the platelet storage lesion that associates morphological and metabolic changes, leading to impaired functions. 3 The conditions of harvesting, processing and storage influence these platelet changes that can be associated with a decrease in post-transfusion recovery in vivo. The platelet storage lesion can be identified by a variety of in vitro tests.…”

Section: Introductionmentioning

confidence: 99%

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Early increase in DcR2 expression and late activation of caspases in the platelet storage lesion

et al. 2001

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Platelet transfusion is widely used to prevent bleeding in patients with severe thrombocytopenia. The maximal storage duration of platelet concentrates is usually 5 days, due to the platelet storage lesion that impairs their functions when stored for longer times. Some of the morphological and biochemical changes that characterize this storage lesion are reminiscent of cell death by apoptosis. The present study analyzed whether proteins involved in nucleated cell apoptosis could play a role in the platelet storage lesion. Storage of leukocyte-depleted platelets obtained by apheresis is associated with a late and limited activation of caspases, mainly caspase-3. This event correlates with an increased expression of the pro-apoptotic BH3-only protein Bim in the particulate fraction and a slight and late release of the pro-apoptotic mitochondrial protein Diablo/Smac in the cytosol. Platelets do not express the death receptors Fas, DR4 and DR5 on their plasma membrane, while the expression of the decoy receptor DcR2 increases progressively during platelet storage. Addition of low concentrations of the cryoprotector dimethylsulfoxide accelerates platelet caspase activation during storage, an effect that is partially prevented by the caspase inhibitor z-VAD-fmk. Altogether, DcR2 expression on the plasma membrane is an early event while caspase activation is a late event during platelet storage. These observations suggest that caspases are unlikely to account for the platelet storage lesion. As a consequence, addition of caspase inhibitors may not improve the quality of platelet concentrates stored in standard conditions. Leukemia (2001) 15, 1572-1581.

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Section: Caspases Are Only Slightly Activated During Platelet Storagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Early increase in DcR2 expression and late activation of caspases in the platelet storage lesion

et al. 2001

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“…It has long been recognized that platelet viability and function decline over time, the so-called "platelet storage lesion." 26,27 The most compelling data pointing to damage incurred during platelet storage come from radiolabeling studies in autologous volunteer platelet donors. Most investigations have shown that platelets transfused following storage have a significantly decreased recovery and survival relative to fresh platelets.…”

Section: Rapid Tests For Bacteriamentioning

confidence: 99%

Platelets: Testing, Dosing and the Storage Lesion—Recent Advances

Kaufman¹

2006

Hematology

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The demand for platelet transfusions continues to grow. Several complementary approaches that may help meet this demand in the future are reviewed. First, platelet bacterial testing is beginning to allow the extension of platelet storage beyond 5 days. Studies are also underway aimed at better preserving viability and function during ex vivo platelet storage: additive solutions and other approaches are being developed to try to negate the "platelet storage lesion." Finally, new approaches to dosing platelets may help extend the limited supply.

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