Quality evaluation of plateletpheresis using the new AMICUS (Baxter) cell separator: Evolution of CD 62 expression

Laurencet, F; Doucet, Arlette; Lydiate, Valmay; Jacquier, Marie-Claude; Mermillod, Bernadette; Andersen, Sylvia; Chapuis, B

doi:10.1002/(sici)1098-1101(1998)13:2<47::aid-jca1>3.0.co;2-8

Cited by 14 publications

(8 citation statements)

References 25 publications

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“…Although not statistically significant, the CD62P expression increased to some extent during storage. Other studies have shown that the percentage of activated (CD62P expressing) platelets increases during storage (Rinder et al, 1993;Holme et al, 1997;Laurencet et al, 1998). Similarly, the changes in morphology and aggregation are similar to those reported during platelet storage in other systems (Glaser et al, 2001).…”

Section: Discussionsupporting

confidence: 77%

Automated collection of blood components: their storage and transfusion

Rock

Moltzan

Alharbi

et al. 2003

Transfusion Medicine

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The performance of an automated device, the Gambro TRIMA, was evaluated for component production, and an in vivo assessment of the platelets was carried out. Red cell concentrates (RCCs), platelets and plasma were collected and stored according to standard blood bank procedures and evaluated for quality by in vitro measurements. Additionally, single-donor platelets (n=10) were transfused to thrombocytopenic patients after 5 days of storage. Platelet counts were measured after 1 h and the corrected count increment (CCI) was calculated. No significant changes were seen before or after procedure in donor haemoglobin, haematocrit, coagulation factors or platelet count. Return-line samples showed no increase in the level of plasma haemoglobin. Plasma haemoglobin and potassium increased following RBC storage, but there was no change in the red cell number. Platelet aggregation decreased from 52 to 11% (adenosine diphosphate) and the Kunicki morphology score dropped from 379 to 174. Little change was seen in the hypotonic shock response (69-63%) or in the percentage of CD62 expression (4.8-19.8) over time. The CCI averaged 28+/-26 x 10(3) microL(-1) in 10 patients 1 h after transfusion. The TRIMA machine collects RCCs, platelets and plasma in a variety of combinations in one session. For autologous collection, two units of RCC plus platelets can be collected at one time, reducing administrative and testing costs. The platelets have good in vivo recovery, as shown by the CCI values. An added advantage is that the TRIMA machine can be used in hospitals to generate components in times of shortage without the need for a component's laboratory.

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Section: Discussionsupporting

confidence: 77%

Automated collection of blood components: their storage and transfusion

Rock

Moltzan

Alharbi

et al. 2003

Transfusion Medicine

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“…All parameters were assayed on the morning of Days 1, 5, and 7 of storage. All metabolic, key storage, and functional parameters were in the range of PLT storage variables published previously 6,18 ‐20 . PLT metabolic parameters were not affected by differences in rest period during storage.…”

Section: Discussionmentioning

confidence: 55%

A rest period before agitation may improve some in vitro apheresis platelet parameters during storage

et al. 2011

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“…It has been previously demonstrated that different cell separators induce different degrees of PLT activation, depending on their collection modalities. [7][8][9]21,[23][24][25] Plateletpheresis, obtained using the surge technology, like those obtained with the Haemonetics MCS device, show lower levels of P-selectin when compared to those obtained using Cobe Spectra or CS-3000 machines. 21 These observations are in keeping with our findings, based on the particular collection modality of the plasma-reduced PLT collection program, where a prolonged contact between high-concentration PLTs and the plastic surface takes place within the collection set.…”

Section: Discussionmentioning

confidence: 96%

“…It has been previously demonstrated that different cell separators induce different degrees of PLT activation, depending on their collection modalities 7‐9 , 21,23‐25 . Plateletpheresis, obtained using the surge technology, like those obtained with the Haemonetics MCS device, show lower levels of P‐selectin when compared to those obtained using Cobe Spectra or CS‐3000 machines 21 .…”

Section: Discussionmentioning

confidence: 99%

Platelet activation during plasma‐reduced multicomponent PLT collection: a comparison between COBE Trima and Spectra LRS turbo cell separators

et al. 2004

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BACKGROUND: The wide diffusion of multicomponent collection in donor apheresis has led to the yielding of different components, such as plasma‐reduced platelet‐pheresis at high PLT concentration. We investigated whether this collection modality could induce more PLT activation compared to standard plateletpheresis. STUDY DESIGN AND METHODS: Forty‐one plateletpheresis collections (20 Trima and 21 Spectra LRS Turbo v.7.0, COBE) were evaluated. Donor, procedure, and product data were recorded. ADP, collagen, and U46619 (a thromboxane‐A2 analog)‐induced PLT aggregation was investigated in basal (donor) and final (plateletpheresis unit) samples. The expression of PLT activation marker P‐selectin (CD62P) was studied using flow cytometry in basal and final samples. In all cases, P‐selectin was investigated in final samples after stimulation with ADP to assess for a possible further release of the antigen. Four additional plateletpheresis procedures were performed in donors from Group A, using the traditional, nonplasma‐reduced program. RESULTS: Plateletpheresis obtained by means of the Trima device showed a lower response to in‐vitro induced PLT aggregation and a higher percentage of P‐selectin‐expressing PLT when compared to products obtained using the Spectra device. Moreover, P‐selectin release after ADP stimulation was reduced in plateletpheresis units obtained using the Trima device. These differences disappeared when a nonplasma‐reduced collection program was used. In‐vivo evaluation did not detect any difference between platelepheresis obtained by means of the two cell separators. CONCLUSIONS: Plateletpheresis units obtained by means of multicomponent collection show a higher degree of PLT activation compared to traditional plateletpheresis procedures when high‐concentration plasma‐reduced products are collected. Randomized clinical studies are needed to assess the real impact of these findings in terms of in‐vivo efficacy of plasma‐reduced plateletpheresis units.

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Quality evaluation of plateletpheresis using the new AMICUS (Baxter) cell separator: Evolution of CD 62 expression

Cited by 14 publications

References 25 publications

Automated collection of blood components: their storage and transfusion

Automated collection of blood components: their storage and transfusion

A rest period before agitation may improve some in vitro apheresis platelet parameters during storage

Platelet activation during plasma‐reduced multicomponent PLT collection: a comparison between COBE Trima and Spectra LRS turbo cell separators

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