Automated collection of blood components: their storage and transfusion

Abstract

The performance of an automated device, the Gambro TRIMA, was evaluated for component production, and an in vivo assessment of the platelets was carried out. Red cell concentrates (RCCs), platelets and plasma were collected and stored according to standard blood bank procedures and evaluated for quality by in vitro measurements. Additionally, single-donor platelets (n=10) were transfused to thrombocytopenic patients after 5 days of storage. Platelet counts were measured after 1 h and the corrected count increment (CCI) was calculated. No significant changes were seen before or after procedure in donor haemoglobin, haematocrit, coagulation factors or platelet count. Return-line samples showed no increase in the level of plasma haemoglobin. Plasma haemoglobin and potassium increased following RBC storage, but there was no change in the red cell number. Platelet aggregation decreased from 52 to 11% (adenosine diphosphate) and the Kunicki morphology score dropped from 379 to 174. Little change was seen in the hypotonic shock response (69-63%) or in the percentage of CD62 expression (4.8-19.8) over time. The CCI averaged 28+/-26 x 10(3) microL(-1) in 10 patients 1 h after transfusion. The TRIMA machine collects RCCs, platelets and plasma in a variety of combinations in one session. For autologous collection, two units of RCC plus platelets can be collected at one time, reducing administrative and testing costs. The platelets have good in vivo recovery, as shown by the CCI values. An added advantage is that the TRIMA machine can be used in hospitals to generate components in times of shortage without the need for a component's laboratory.

The quality of platelet concentrates produced by COBE Spectra and Trima Accel cell separators during storage for 7 days as assessed by in vitro methods

“…HSR, a variable that has been shown to correlate with in vivo survival, 30 indicated that the PLT quality was well maintained for 7 days of storage. Rock and coworkers 31 showed that HSR remained stable for 5 days in PCs produced by the Trima v.4.0 but at a slightly lower level (60%‐70%) compared to our study (80%). Dumont and colleagues 26 showed reduction in HSR by 15 percent during 7 days of storage to approximately 50 percent in PCs produced by the Spectra, which is in contrast to our results.…”

Metabolic effects occurring in irradiated and non-irradiated red blood cellular components for clinical transfusion practice: An in vitro comparison

“…RBC products can be obtained by processing a WB donation by either using a centrifugation or apheresis method. 10,11,12 In South Africa, the standard procedure used is the centrifugation method. The plasma and buffy coat are extracted and distributed between the sterile, interconnected but separate satellite bags.…”