The plasma membrane protein Rch1 is a negative regulator of cytosolic calcium homeostasis and positively regulated by the calcium/calcineurin signaling pathway in budding yeast

Zhao, Yang; Yan, Hongbo; Happeck, Ricardo; Peiter-Volk, Tina; Xu, Huihui; Yan, Zhang; Peiter, Edgar; Triplet, Chloë van Oostende; Whiteway, Malcolm; Jiang, Lijing

doi:10.1016/j.ejcb.2016.01.001

Cited by 31 publications

(24 citation statements)

References 40 publications

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“…We confirmed that overexpression of ScRSN1 in the µ plasmid pHAC181 suppressed the sodium sensitivity of S. cerevisiae cells lacking ScSRO7, but overexpression of the orf19.4805 did not ( Supplementary Fig. 4; Zhao et al 2016;Li et al 2008). This suggests that the orf19.4805 could not complement the function of ScRsn1 in suppressing the sodium sensitivity of cells lacking ScSRO7, indicating that the orf19.4805 is not a functional homolog of ScRsn1.…”

Section: Resultssupporting

confidence: 61%

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The putative transient receptor potential channel protein encoded by the orf19.4805 gene is involved in cation sensitivity, antifungal tolerance, and filamentation inCandida albicans

Jiang

Yang

2018

Can. J. Microbiol.

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Transient receptor potential (TRP) channels, an ancient family of cation channels, are highly conserved in eukaryotes and play various physiological functions, ranging from sensation of ion homeostasis to reception of pain and vision. Calcium-permeable TRP channels have been identified from the plant Arabidopsis thaliana (AtCsc1) and the budding yeast Saccharomyces cerevisiae (ScCsc1). In this study, we characterized the functions of the Csc1 homolog, orf19.4805, in Candida albicans. Orf19.4805 is a protein of 866 amino acids and 11 transmembrane domains, which shares 49% identity (69% similarity) in amino acid sequence with ScRsn1. Here, we demonstrate that deletion of the orf19.4805 gene causes C. albicans cells to be sensitive to SDS (sodium dodecyl sulfate) and antifungal drugs, and tolerance to zinc, manganese, and cadmium ions. Candida albicans cells lacking orf19.4805 show a defect in filamentation in vitro. Therefore, orf19.4805 is involved in the regulation of cation homeostasis and filamentation in C. albicans.

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Section: Resultssupporting

confidence: 61%

“…In eukaryotic cells, one of the earliest responses to stresses is the elevation of cytosolic calcium concentration (Swarbreck et al 2013;Peiter et al 2016;Zhao et al 2016;). Calcium-permeable transient receptor potential (TRP) channels are present in yeasts, animals and plants (Chang et al 2010;Yuan et al 2014;Hou et al 2014).…”

Section: Introductionmentioning

confidence: 99%

The putative transient receptor potential channel protein encoded by the orf19.4805 gene is involved in cation sensitivity, antifungal tolerance, and filamentation inCandida albicans

Jiang

Yang

2018

Can. J. Microbiol.

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“…In Saccharomyces cerevisiae, an increase in cytosolic calcium triggers the calmodulin/Ca 2+ binding and activation of the protein phosphatase, calcineurin. Activated calcineurin dephosphorylates ScCrz1 in the cytosol, which leads to nuclear localization of ScCrz1 and its binding to promoters of its target genes, including the vacuolar calcium pump gene ScPMC1, the ER/Golgi calcium pump gene ScPMR1 and the ScRCH1 gene encoding the negative regulator of calcium uptake in the plasma membrane [8][9][10]. A genome-scale genetic screen has revealed additional genes that are involved in the regulation of calcium homeostasis in budding yeast [11].…”

Section: Backgoundmentioning

confidence: 99%

RNA sequencing reveals an additional Crz1-binding motif in promoters of its target genes in the human fungal pathogen Candida albicans

et al. 2020

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Background: The calcium/calcineurin signaling pathway is mediated by the transcription factors NFAT (nuclear factor of activated T cells) in mammals and Crz1 (calcineurin-responsive zinc finger 1) in yeasts and other lower eukaryotes. A previous microarray analysis identified a putative Crz1-binding motif in promoters of its target genes in Candida albicans, but it has not been experimentally demonstrated. Methods: An inactivation mutant for CaCRZ1 was generated through CRISPR/Cas9 approach. Transcript profiling was carried out by RNA sequencing of the wild type and the inactivation mutant for CaCRZ1 in response to 0.2 M CaCl 2 . Gene promoters were scanned by the online MEME (Multiple Em for Motif Elicitation) software. Gel electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) analysis were used for in vitro and in vivo CaCrz1-binding experiments, respectively.

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“…Functional counterparts of Ca 2+ transporters and channels have been characterized in Candida albicans [ 8 – 11 ]. Rch1 is a novel negative regulator of calcium uptake in the plasma membrane of S. cerevisiae and C. albicans [ 12 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

CaGdt1 plays a compensatory role for the calcium pump CaPmr1 in the regulation of calcium signaling and cell wall integrity signaling in Candida albicans

et al. 2018

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BackgroundSaccharomyces cerevisiae ScGdt1 and mammalian TMEM165 are two members of the UPF0016 membrane protein family that is likely to form a new group of Ca2+/H+ antiporter and/or a Mn2+ transporter in the Golgi apparatus. We have previously shown that Candida albicans CaGDT1 is a functional ortholog of ScGDT1 in the response of S. cerevisiae to calcium stress. However, how CaGdt1 together with the Golgi calcium pump CaPmr1 regulate calcium homeostasis and cell wall integrity in this fungal pathogen remains unknown.MethodsChemical sensitivity was tested by dilution assay. Cell survival was examined by measuring colony-forming units and staining with Annexin V-FITC and propidium iodide. Calcium signaling was examined by expression of downstream target gene CaUTR2, while cell wall integrity signaling was revealed by detection of phosphorylated Mkc1 and Cek1. Subcellular localization of CaGdt1 was examined through direct and indirect immunofluorescent approaches. Transcriptomic analysis was carried out with RNA sequencing.ResultsThis study shows that Candida albicans CaGDT1 is also a functional ortholog of ScGDT1 in the response of S. cerevisiae to cell wall stress. CaGdt1 is localized in the Golgi apparatus but at distinct sites from CaPmr1 in C. albicans. Loss of CaGDT1 increases the sensitivity of cell lacking CaPMR1 to cell wall and ER stresses. Deletion of CaGDT1 and/or CaPMR1 increases calcium uptake and activates the calcium/calcineurin signaling. Transcriptomic profiling reveals that core functions shared by CaGdt1 and CaPmr1 are involved in the regulation of cellular transport of metal ions and amino acids. However, CaGdt1 has distinct functions from CaPmr1. Chitin synthase gene CHS2 is up regulated in all three mutants, while CHS3 is only up regulated in the pmr1/pmr1 and the gdt1/gdt1 pmr1/pmr1 mutants. Five genes (DIE2, STT3, OST3, PMT1 and PMT4) of glycosylation pathway and one gene (SWI4) of the cell wall integrity (CWI) pathway are upregulated due to deletion of CaGDT1 and/or CaPMR1. Consistently, deletion of either CaPMR1 or CaGDT1 activates the CaCek1-mediated CWI signaling in a cell wall stress-independent fashion. Calcineurin function is required for the integrity of the cell wall and vacuolar compartments of cells lacking both GDT1 and CaPMR1.ConclusionsCaPmr1 is the major player in the regulation of calcium homeostasis and cell wall stress, while CaGdt1 plays a compensatory role for CaPmr1 in the Golgi compartment in C. albicans.Electronic supplementary materialThe online version of this article (10.1186/s12964-018-0246-x) contains supplementary material, which is available to authorized users.

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The plasma membrane protein Rch1 is a negative regulator of cytosolic calcium homeostasis and positively regulated by the calcium/calcineurin signaling pathway in budding yeast

Cited by 31 publications

References 40 publications

The putative transient receptor potential channel protein encoded by the orf19.4805 gene is involved in cation sensitivity, antifungal tolerance, and filamentation inCandida albicans

The putative transient receptor potential channel protein encoded by the orf19.4805 gene is involved in cation sensitivity, antifungal tolerance, and filamentation inCandida albicans

RNA sequencing reveals an additional Crz1-binding motif in promoters of its target genes in the human fungal pathogen Candida albicans

CaGdt1 plays a compensatory role for the calcium pump CaPmr1 in the regulation of calcium signaling and cell wall integrity signaling in Candida albicans

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