The plant non-specific phospholipase C gene family. Novel competitors in lipid signalling

Pokotylo, Igor; Pejchar, Přemysl; Potocký, Martin; Kocourková, Daniela; Krčková, Zuzana; Ruelland, Éric; Kravets, V. S.; Martinec, Jan

doi:10.1016/j.plipres.2012.09.001

Cited by 98 publications

(99 citation statements)

References 211 publications

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“…ZmPLC1 from maize ( Zea mays L .) is up-regulated in response to dehydration [50], and enhanced expression of sense ZmPLC1 has been shown to improve the drought tolerance of maize plants [19], [51]. In this study, we found that TaPLC1 expression in wheat was induced in response to high salt and drought stress at both the mRNA and protein levels.…”

Section: Discussionmentioning

confidence: 60%

Expression Analysis of a Stress-Related Phosphoinositide-Specific Phospholipase C Gene in Wheat (Triticum aestivum L.)

Zhang

Jin

et al. 2014

PLoS ONE

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Plant phosphoinositide-specific phospholipases C (PI-PLCs) function in several essential plant processes associated with either development or environmental stress. In this report, we examined the expression patterns of TaPLC1 under drought and high salinity stress at the transcriptional and post-transcriptional levels. TaPLC1 mRNA was expressed in all wheat organs examined. U73122 and edelfosine, the PLC inhibitor, impaired seedling growth and enhanced seedling sensitivity to drought and high salinity stress. Though TaPLC1 expression in wheat was lowest at the seedling stage, it was strongly induced under conditions of stress. When 6-day-old wheat seedlings were treated with 200 mM NaCl or 20% (w/v) PEG 6000 for 6 or 12 h, respectively, the TaPLC1 transcript level increased by 16-fold compared to the control. Western blotting showed that the TaPLC protein concentration was also maintained at a high level from 24 to 48 h during stress treatment. Together, our results indicate the possible biological functions of TaPLC1 in regulating seedling growth and the response to drought and salinity stress.

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Section: Discussionmentioning

confidence: 60%

Expression Analysis of a Stress-Related Phosphoinositide-Specific Phospholipase C Gene in Wheat (Triticum aestivum L.)

Zhang

Jin

et al. 2014

PLoS ONE

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“…We found that DEGs showing similarity to, inter alia , non-specific phospholipase C (NPC), belonged to this pathway. NPCs play important roles in many processes as phospholipid-to-galactosyl DAG exchange, growth and development associated with hormone signaling and stress responses (reviewed in: Pokotylo et al, 2013). The same phospholipases were included in the “Ether lipid metabolism” and “Glycerophospholipid metabolism” pathways that were strongly up-regulated in the abscising flower pedicels.…”

Section: Discussionmentioning

confidence: 99%

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

et al. 2017

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Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant's productivity. Generative organ detachment takes place at the basis of the pedicels, within a specialized group of cells collectively known as the abscission zone (AZ). During plant growth these cells become competent to respond to specific signals that trigger separation and lead to the abolition of cell wall adhesion. Little is known about the molecular network controlling the yellow lupine organ abscission. The aim of our study was to establish the divergences and similarities in transcriptional networks in the pods, flowers and flower pedicels abscised or maintained on the plant, and to identify genes playing key roles in generative organ abscission in yellow lupine. Based on de novo transcriptome assembly, we identified 166,473 unigenes representing 219,514 assembled unique transcripts from flowers, flower pedicels and pods undergoing abscission and from control organs. Comparison of the cDNA libraries from dropped and control organs helped in identifying 1,343, 2,933 and 1,491 differentially expressed genes (DEGs) in the flowers, flower pedicels and pods, respectively. In DEG analyses, we focused on genes involved in phytohormonal regulation, cell wall functioning and metabolic pathways. Our results indicate that auxin, ethylene and gibberellins are some of the main factors engaged in generative organ abscission. Identified 28 DEGs common for all library comparisons are involved in cell wall functioning, protein metabolism, water homeostasis and stress response. Interestingly, among the common DEGs we also found an miR169 precursor, which is the first evidence of micro RNA engaged in abscission. A KEGG pathway enrichment analysis revealed that the identified DEGs were predominantly involved in carbohydrate and amino acid metabolism, but some other pathways were also targeted. This study represents the first comprehensive transcriptome-based characterization of organ abscission in L. luteus and provides a valuable data source not only for understanding the abscission signaling pathway in yellow lupine, but also for further research aimed at improving crop yields.

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“…Two types of PLCs are known: non-specific PLC (NPC) and phosphoinositide-specific PLC (PI-PLC). NPC hydrolyzes major phospholipid classes such as PC and PE and is found only in bacteria [90] and higher plants [91,92]. PI-PLC specifically hydrolyzes PI or its phosphorylated derivatives (phosphoinositides, PIPn) such as PI 4,5-bisphosphate (PI(4,5)P 2 ).…”

Section: Phospholipase C (Plc)mentioning

confidence: 99%

Function of polar glycerolipids in flower development in Arabidopsis thaliana

Nakamura

2015

Progress in Lipid Research

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The plant non-specific phospholipase C gene family. Novel competitors in lipid signalling

Cited by 98 publications

References 211 publications

Expression Analysis of a Stress-Related Phosphoinositide-Specific Phospholipase C Gene in Wheat (Triticum aestivum L.)

Expression Analysis of a Stress-Related Phosphoinositide-Specific Phospholipase C Gene in Wheat (Triticum aestivum L.)

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

Function of polar glycerolipids in flower development in Arabidopsis thaliana

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