Expression Analysis of a Stress-Related Phosphoinositide-Specific Phospholipase C Gene in Wheat (Triticum aestivum L.)

Abstract

Plant phosphoinositide-specific phospholipases C (PI-PLCs) function in several essential plant processes associated with either development or environmental stress. In this report, we examined the expression patterns of TaPLC1 under drought and high salinity stress at the transcriptional and post-transcriptional levels. TaPLC1 mRNA was expressed in all wheat organs examined. U73122 and edelfosine, the PLC inhibitor, impaired seedling growth and enhanced seedling sensitivity to drought and high salinity stress. Though TaPLC1 expression in wheat was lowest at the seedling stage, it was strongly induced under conditions of stress. When 6-day-old wheat seedlings were treated with 200 mM NaCl or 20% (w/v) PEG 6000 for 6 or 12 h, respectively, the TaPLC1 transcript level increased by 16-fold compared to the control. Western blotting showed that the TaPLC protein concentration was also maintained at a high level from 24 to 48 h during stress treatment. Together, our results indicate the possible biological functions of TaPLC1 in regulating seedling growth and the response to drought and salinity stress.

Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat

“…The PLC/DGK pathway is one of the most important signaling networks in response to biotic and abiotic stresses [1,5,18]. Our previous work revealed the function of TaPLC1 in controlling seedling growth and adapting to drought and salt stress [22], while the present study is focused on the role of DGK genes in wheat.…”

“…Additionally, overexpression of the wheat phospholipase D gene TaPLDα has been shown to enhance tolerance to drought and osmotic stress in transgenic Arabidopsis thaliana [3]. Previously, we had measured the TaPLC expression patterns at both the transcriptional and protein levels in response to drought and salinity stress, and our research indicated the possible roles of TaPLC1 in mediating seedling growth and responding to drought and salinity stress [22]. However, the molecular functions of lipid messengers remain unclear in wheat.…”

“…The wheat seeds (Chinese Spring) ware provided by professor Yanyun Pan (Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology) [22]. Seeds were brie y surface-sterilized with ethanol (70%), then that were immersed in bleach solution (30%) for 10 min.…”

Arabidopsis phosphoinositide‐specific phospholipase C 4 negatively regulates seedling salt tolerance

“…The production of lipids and lipid derivatives is controlled by different families of enzymes, including phospholipases, lipid kinases and phosphatases (Wang 2004;). PI-PLC activity and its enzymatic products (IP 3 and DAG) are involved in regulating the plant salt stress response; Tripathy et al 2012;Zhang et al 2014;Zhang et al 2015). Among these PI-PLC genes, the roles of NtPLCδ1 in salt and osmotic stresses responses (Tripathy et al 2012), AtPLC1 in ABA signal secondary responses (Sanchez & Chua 2001, and AtPLC5 in leaf senescence (Zhang et al 2015) have been confirmed.…”

“…PI‐PLC activity and its enzymatic products (IP 3 and DAG) are involved in regulating the plant salt stress response; however, which member(s) of the PI‐PLC family is responsible and how it regulates the salt stress response remain largely unknown. The transcription of multiple PI‐PLC genes, such as AtPLC1 , 3 , 4‐7 in Arabidopsis, NtPLCδ1 in tobacco ( N. tobacco ), VrPLC3 in mung bean (Vigna radiate) , PtoPLC1 in poplar ( Populus tomentosa ) and TaPLC1 in wheat ( Triticum aestivum ), is significantly induced by salt and other abiotic or biotic stresses (Hirayama et al ; Kim et al ; Tasma et al ; Tripathy et al ; Zhang et al ; Zhang et al ). Among these PI‐PLC genes, the roles of NtPLCδ1 in salt and osmotic stresses responses (Tripathy et al ), AtPLC1 in ABA signal secondary responses (Sanchez & Chua , and AtPLC5 in leaf senescence (Zhang et al ) have been confirmed.…”