The Plant NADPH Oxidase RBOHD Mediates Rapid Systemic Signaling in Response to Diverse Stimuli

Miller, Gad; Schlauch, Karen; Tam, Rachel; Cortes, Diego Fernando Marmolejo; Torres, Miguel Ángel Medina; Shulaev, Vladimir; Dangl, Jeffery L.; Mittler, Ron

doi:10.1126/scisignal.2000448

Cited by 896 publications

(1,010 citation statements)

References 38 publications

Supporting

Mentioning

959

Contrasting

Unclassified

Order By: Relevance

“…We suggest that this non-ROS signal is destined to activate AtrbohD and AtrbohF NADPH oxidases at the plasma membrane. The involvement of AtrbohD/F and a strong rapid accumulation of extracellular H 2 O 2 in high-light signaling has been observed (Davletova et al, 2005;Bechtold et al, 2008;Galvez-Valdivieso et al, 2009;Miller et al, 2009). As with the involvement of AtrbohD and AtrbohF in ABA signaling in guard cells (Kwak et al, 2003;Miao et al, 2006), we suggest that H 2 O 2 produced at the plasma membrane could oxidize glutathione peroxidase isoforms, which in turn would bind to and inhibit ABI1 and ABI2 in BSCs.…”

Section: Systemic Signalingmentioning

confidence: 94%

“…How signal transduction is mediated from the burst of AtrbohDcatalyzed ROS to signaling pathways is not known, but it could involve glutathione peroxidases, as described for abscisic acid (ABA)-mediated signaling (see below). AtrobhD is also central to localized cell-tocell signal propagation, presumably by providing apoplastic O 2 .2 , which dismutates in the apoplast to H 2 O 2 (Miller et al, 2009). The effect of such localized H 2 O 2 signal propagation is to contain the spread of the HR/photooxidative stress-induced lesion by raising the threshold of cell death initiation by boosting antioxidant defenses in adjacent cells.…”

Section: Ros Signaling In Cells Neighboring Cell Death Lesionsmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative Stress: Antagonistic Signaling for Acclimation or Cell Death?

2010

View full text Add to dashboard Cite

Severe environmental stress imposed on plant tissues induces changes in oxygen (O 2 ) metabolism that cause oxidative stress. Oxidative stress occurs when reactive oxygen species (ROS) are not rapidly scavenged and the rate of repair of damaged cell components fails to keep pace with the rate of damage. If this situation persists, irreversible damage results in a loss of physiological competence and eventual cell death. However, ROS production in leaves resulting from moderate environmental stresses, within the adaptive range of the plant, also has important local and systemic signaling roles. In these circumstances, ROS production induces defense mechanisms that protect the plant but do not result in oxidative stress. We shall illustrate ROS involvement in signaling in both of these situations by considering the role of chloroplasts in initiating cellular responses to environmental perturbations, choosing examples where this organelle interacts with specific signaling pathways.

show abstract

Section: Systemic Signalingmentioning

confidence: 94%

Section: Ros Signaling In Cells Neighboring Cell Death Lesionsmentioning

confidence: 99%

Oxidative Stress: Antagonistic Signaling for Acclimation or Cell Death?

2010

View full text Add to dashboard Cite

show abstract

“…However, Rboh activity may play a broader role in syncytium development and function. Moreover, the fact that RbohD mediates systemic signaling in response to diverse stimuli (23) suggests that ROS could be involved in systemic signaling between nurse cells and the host plant.…”

Section: R E S E a R C H A R T I C L Ementioning

confidence: 99%

“…S2, A to D), suggesting that 35S::RbohD complemented the rbohD mutation. Second, we treated plants with the compound diphenyliodonium (DPI), which inhibits Rboh activity (23,24). Col-0 plants treated with DPI had fewer invaded nematodes 14 dai compared to untreated control plants (Fig.…”

Section: Rbohd and Rbohf Promote Nematode Parasitismmentioning

confidence: 99%

Parasitic Worms Stimulate Host NADPH Oxidases to Produce Reactive Oxygen Species That Limit Plant Cell Death and Promote Infection

et al. 2014

View full text Add to dashboard Cite

Plants and animals produce reactive oxygen species (ROS) in response to infection. In plants, ROS not only activate defense responses and promote cell death to limit the spread of pathogens but also restrict the amount of cell death in response to pathogen recognition. Plants also use hormones, such as salicylic acid, to mediate immune responses to infection. However, there are long-lasting biotrophic plant-pathogen interactions, such as the interaction between parasitic nematodes and plant roots during which defense responses are suppressed and root cells are reorganized to specific nurse cell systems. In plants, ROS are primarily generated by plasma membrane-localized NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) oxidases, and loss of NADPH oxidase activity compromises immune responses and cell death. We found that infection of Arabidopsis thaliana by the parasitic nematode Heterodera schachtii activated the NADPH oxidases RbohD and RbohF to produce ROS, which was necessary to restrict infected plant cell death and promote nurse cell formation. RbohD-and RbohF-deficient plants exhibited larger regions of cell death in response to nematode infection, and nurse cell formation was greatly reduced. Genetic disruption of SID2, which is required for salicylic acid accumulation and immune activation in nematode-infected plants, led to the increased size of nematodes in RbohD-and RbohF-deficient plants, but did not decrease plant cell death. Thus, by stimulating NADPH oxidase-generated ROS, parasitic nematodes fine-tune the pattern of plant cell death during the destructive root invasion and may antagonize salicylic acid-induced defense responses during biotrophic life stages.

show abstract

“…The triggering of these oxygen intermediates leads to the activation of defence mechanisms in the plant cells, which consist of antioxidants, such as ascorbate and glutathione, and enzymatic antioxidant activities such as CIII-POX, CAT, SOD and the ascorbate-glutathione cycle enzymes, ascorbate peroxidase (APOX) and GR (Apel and Hirt, 2004;Passardi et al, 2005;Gill and Tuteja, 2010). Additionally, stress-induced ROS can act as signaling molecules specifically inducing several cell and molecular responses (Miller et al, 2009(Miller et al, , 2010Kärkönen and Kuchitsu, 2015).…”

Section: Discussionmentioning

confidence: 99%

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Largo-Gosens

Castro

Alonso‐Simón

et al. 2016

Plant Physiology and Biochemistry

View full text Add to dashboard Cite

The habituation of bean cells to quinclorac did not rely on cell wall modifications, contrary to what it was previously observed for the well-known cellulose biosynthesis inhibitors dichlobenil or isoxaben. The aim of the present study was to investigate whether or not the bean cells habituation to quinclorac is related to an enhancement of antioxidant activities involved in the scavenging capacity of reactive oxygen species. Treating non-habituated bean calluses with 10 μM quinclorac reduced the relative growth rate and induced a two-fold increase in lipid peroxidation. However, the exposition of quinclorac-habituated cells to a concentration of quinclorac up to 30 μM neither affected their growth rate nor increased their lipid peroxidation levels. Quinclorac-habituated calluses had significantly higher constitutive levels of three antioxidant activities (class-III peroxidase, glutathione reductase, and superoxide dismutase) than those observed in non-habituated calluses, and the treatment of habituated calluses with 30 μM quinclorac significantly increased the level of class III-peroxidase and superoxide dismutase. The results reported here indicate that the process of habituation to quinclorac in bean callus-cultured cells is related, at least partially, to the development of a stable antioxidant capacity that enables them to cope with the oxidative stress caused by quinclorac. Class-III peroxidase and superoxide dismutase activities could play a major role in the quinclorac-habituation. Changes in the antioxidant status of bean cells were stable, since the increase in the antioxidant activities were maintained in quinclorac-dehabituated cells.

show abstract

The Plant NADPH Oxidase RBOHD Mediates Rapid Systemic Signaling in Response to Diverse Stimuli

Cited by 896 publications

References 38 publications

Oxidative Stress: Antagonistic Signaling for Acclimation or Cell Death?

Oxidative Stress: Antagonistic Signaling for Acclimation or Cell Death?

Parasitic Worms Stimulate Host NADPH Oxidases to Produce Reactive Oxygen Species That Limit Plant Cell Death and Promote Infection

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Contact Info

Product

Resources

About