The plant lectin soybean agglutinin binds to the soma, axon and central terminals of a subpopulation of small-diameter primary sensory neurons in the rat and cat

Plenderleith, Mark B.; Cameron, Andrew R.; Key, Brian; Snow, Peter J.

doi:10.1016/0306-4522(89)90433-8

Cited by 49 publications

(26 citation statements)

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“…In our previous study, we observed substance P immunoreactivity, a marker of nociceptive primary afferent fibers (Levine et al, 1993;Lawson et al, 1997;Todd et al, 2002), in many VGluT1-and VGluT2-immunoreactive axon terminals in lamina I and outer part of lamina II of the medullary dorsal horn (Li et al, 2003). We also observed that the plant lectin Bandeiraea simplicifolia I-B 4 , which has been reported to bind specifically to unmyelinated axons (Streit et al, 1985;Plenderleith et al, 1989;Plenderleith and Snow, 1993), often bound to VGluT2-immunoreactive axon terminals but rarely to VGluT1-immunoreactive ones in lamina II of the medullary dorsal horn (Li et al, 2003). Thus, the present results and those of our previous study (Li et al, 2003) indicated that nociceptive primary afferent neurons express VGluT2 more frequently than VGluT1.…”

Section: Discussionmentioning

confidence: 77%

Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression

Xiong

Dong

et al. 2003

J of Comparative Neurology

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Vesicular glutamate transporters are responsible for glutamate transport into synaptic vesicles. In the present study, we examined immunohistochemically the expression of vesicular glutamate transporters, VGluT1 and VGluT2, in trigeminal ganglion neurons of the rat. Immunohistochemistry for VGluT1 and VGluT2 indicated that more than 80% of trigeminal ganglion neurons express VGluT1 and/or VGluT2 in their cell bodies. It also indicated that large and small trigeminal ganglion neurons express VGluT2 more frequently than VGluT1. Dual immunofluorescence histochemistry for VGluT1 and VGluT2 indicated that trigeminal ganglion neurons express VGluT2 more frequently than VGluT1 and that more than 80% of VGluT-expressing trigeminal ganglion neurons express VGluT1 and VGluT2. Many axon terminals in the superficial layers of the medullary dorsal horn also showed VGluT1 and VGluT2 immunoreactivities. Some of these axon terminals were confirmed to form the central core of the synaptic glomerulus. These results indicated that VGluT1 and VGluT2 are coexpressed in the cell bodies and axon terminals in most trigeminal ganglion neurons.

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Section: Discussionmentioning

confidence: 77%

Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression

Xiong

Dong

et al. 2003

J of Comparative Neurology

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“…To obtain information about the proportion and size distribution of sensory neurones expressing lectin binding, we carried out a morphometric analysis of lectin-positive and lectin-negative cells in each of the species. The morphometric analysis was previously described in detail (Plenderleith et al, 1989), but, briefly, it involved measuring the mean diameter of all lectinlabelled and -unlabelled cells in one section from two or three specimens of every species and then constructing a frequency distribution histogram showing both labelled and unlabelled cells. For each species, statistical analysis of the mean diameter of lectin-labelled and -unlabelled cells was performed using the two-tailed Student's t-test.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Gerke

Plenderleith

2002

Anat. Rec.

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The purpose of the present study was to investigate the binding patterns of the plant lectin Bandeiraea simplicifolia I-isolectin B 4 (BSI-B 4 ) to sensory neurones in seven mammalian species. The dorsal root ganglia and spinal cords of three rats, mice, guinea pigs, rabbits, flying foxes, cats, and marmoset monkeys were screened for BSI-B 4 using lectin histochemistry. BSI-B 4 binding was associated with the soma of predominantly smalldiameter primary sensory neurones in the dorsal root ganglia and their axon terminals within laminae I and II of the superficial dorsal horn in all seven species. The similarities of lectin binding patterns in each of these species suggest that the glycoconjugate to which BSI-B 4 binds has a ubiquitous distribution in mammals, and supports the proposal that this lectin may preferentially bind to a subpopulation of sensory neurones with a similar functional role in each of these species. Anat Rec 268: 105-114, 2002.

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“…SBA binds specifically to carbohydrate residues containing N-acetyl D-galactosamine, and it has been shown that the addition of N-acetyl D-galactosamine to cultured explants of sensory neurons and spinal cord causes the preferential growth of sensory neurons toward the dorsal spinal cord (Baker, 1983). Membrane glycoconjugates that bind this lectin have been demonstrated in DRG cells and in unmyelinated central processes in the rat and cat (Plenderleith et al, 1989). Interestingly, a recent study showed that SBA staining is increased in human spinal motor neurons in subacute motor neuropathy associated with lymphoma (Nagao et al, 1990).…”

Section: Changes In Growth-related Substancesmentioning

confidence: 97%

Time course of degenerative and regenerative changes in the dorsal horn in a rat model of peripheral neuropathy

et al. 1997

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The time course of histochemical changes in the dorsal horn of rats subjected to an experimental peripheral neuropathy has been examined. Qualitative and quantitative analyses of the changes in dorsal horn staining were made for soybean agglutinin (SBA)-binding glycoconjugates, the soluble lectins RL-14.5 and RL-29, the growth-associated protein (GAP)-43, and the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP). These analyses were made at various time points after chronic constriction of the sciatic nerve. Quantitative analysis indicated that staining density increased in the normal territories stained for SBA-binding glycoconjugates, RL-14.5, RL-29, and GAP-43 on the neuropathic side compared with the control side. In addition, there was an extension of the territories stained for SBA-binding glycoconjugates and RL-29 ipsilateral to the injury. The peak increases occurred at 14 or 28 days, followed by a decrease toward control levels by 70 days. In contrast, the staining density for SP in the ipsilateral dorsal horn decreased at 3 and 5 days and reached a peak decrease at 14 days. Then, the staining for SP returned toward control values. The staining for CGRP was unchanged at all time points examined. Dorsal rhizotomies ipsilateral to the nerve injury in neuropathic rats indicated that the increases in staining were attributable to changes in primary afferent neurons. These data suggest that peripheral neuropathy causes complex degenerative and regenerative changes in the central branches of primary afferent neurons. The associated synaptic reorganization may contribute to the sensory abnormalities that accompany peripheral neuropathy.

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The plant lectin soybean agglutinin binds to the soma, axon and central terminals of a subpopulation of small-diameter primary sensory neurons in the rat and cat

Cited by 49 publications

References 50 publications

Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression

Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Time course of degenerative and regenerative changes in the dorsal horn in a rat model of peripheral neuropathy

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The plant lectin soybean agglutinin binds to the soma, axon and central terminals of a subpopulation of small-diameter primary sensory neurons in the rat and cat

Cited by 49 publications

References 50 publications

Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression

Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B4 on primary sensory neurones in seven mammalian species

Time course of degenerative and regenerative changes in the dorsal horn in a rat model of peripheral neuropathy

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Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species