Analysis of the distribution of binding sites for the plant lectin <i>Bandeiraea simplicifolia</i> I‐isolectin B<sub>4</sub> on primary sensory neurones in seven mammalian species

Gerke, Michelle B.; Plenderleith, Mark B.

doi:10.1002/ar.10144

Cited by 23 publications

(24 citation statements)

References 63 publications

(52 reference statements)

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“…The cause of this is currently unknown. These cells could represent endothelial cells not associated with vasculature that are most likely to have arisen before the progression into atresia or the acquisition of lectin binding domains by nonendothelial cells as observed previously [26,27]. Irrespective of this additional set of cells in basal atretic follicles that bind lectin, the vasculature in the theca interna clearly is oriented differently in these follicles.…”

Section: Discussionmentioning

confidence: 77%

Theca Interna: The Other Side of Bovine Follicular Atresia1

Clark

Irving-Rodgers

Dharmarajan

et al. 2004

Biology of Reproduction

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Currently, histological classifications of ovarian follicular atresia are almost exclusively based on the morphology of the membrana granulosa without reference to the theca interna. Atresia in the bovine small antral ovarian follicle has been redefined into antral or basal atresia where cell death commences initially within antral or basal regions of the membrana granulosa, respectively. To examine cell death in the theca interna in the two types of atretic follicles, bovine ovaries were collected and processed for immunohistochemistry and light microscopy. Follicles were classified as healthy, antral atretic, or basal atretic. Follicle diameter was recorded and sections stained with lectin from Bandeiraea simplicifolia to identify endothelial cells or with an antibody to cytochrome P450 cholesterol side-chain cleavage to identify steroidogenic cells and combined with TUNEL labeling to identify dead cells. The numerical density of steroidogenic cells within the theca interna was significantly reduced (P < 0.001) in basal atretic follicles in comparison with other follicles. Cell death was greater in both endothelial cells (P < 0.05) and steroidogenic cells (P < 0.01) of the theca interna of basal atretic follicles compared with healthy and antral atretic follicles. Thus, we conclude that the theca interna is susceptible to cell death early in atresia, particularly in basal atretic follicles.

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Section: Discussionmentioning

confidence: 77%

Theca Interna: The Other Side of Bovine Follicular Atresia1

Clark

Irving-Rodgers

Dharmarajan

et al. 2004

Biology of Reproduction

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“…The density of GAD65-IR terminals was also quantified in LI (Figure 4C), a region which is much less innervated by non-peptidergic fibres [10, 29, 30]. From 5 days, the number of GAD65-IR varicosities progressively declined and reached a minimum at 21 days, when their density ipsilateral to injury was 63.1 ± 7.7% of sham controls.…”

Section: Resultsmentioning

confidence: 99%

Spatial and Temporal Pattern of Changes in the Number of GAD65-Immunoreactive Inhibitory Terminals in the Rat Superficial Dorsal Horn following Peripheral Nerve Injury

et al. 2014

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Inhibitory interneurons are an important component of dorsal horn circuitry where they serve to modulate spinal nociception. There is now considerable evidence indicating that reduced inhibition in the spinal dorsal horn contributes to neuropathic pain. A loss of these inhibitory neurons after nerve injury is one of the mechanisms being proposed to account for reduced inhibition; however, this remains controversial. This is in part because previous studies have focused on global measurements of inhibitory neurons without assessing the number of inhibitory synapses. To address this, we conducted a quantitative analysis of the spatial and temporal changes in the number of inhibitory terminals, as detected by glutamic acid decarboxylase 65 (GAD65) immunoreactivity, in the superficial dorsal horn of the spinal cord following a chronic constriction injury (CCI) to the sciatic nerve in rats. Isolectin B4 (IB4) labelling was used to define the location within the dorsal horn directly affected by the injury to the peripheral nerve. The density of GAD65 inhibitory terminals was reduced in lamina I (LI) and lamina II (LII) of the spinal cord after injury. The loss of GAD65 terminals was greatest in LII with the highest drop occurring around 3–4 weeks and a partial recovery by 56 days. The time course of changes in the number of GAD65 terminals correlated well with both the loss of IB4 labeling and with the altered thresholds to mechanical and thermal stimuli. Our detailed analysis of GAD65+ inhibitory terminals clearly revealed that nerve injury induced a transient loss of GAD65 immunoreactive terminals and suggests a potential involvement for these alterations in the development and amelioration of pain behaviour.

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“…Laminae I and II of the dorsal horn did not display WFAbinding sites in the mouse (Figs. 4A and 5A), whereas in the rat they showed an intense WFA labeling that, however, had not the morphological features of extracellular matrix components, but instead those of binding sites localized on primary afferents fibers and consisting of puncta and varicose filaments (Gerke and Plenderleith, 2002). Binding of WFA to primary afferent fibers was confirmed by its extended colocalization with SP, the neurotransmitter of axonal projections deriving from smalldiameter primary sensory neurons (not shown).…”

Section: Light and Confocal Microscopy Immunohistochemistrymentioning

confidence: 88%

Distribution of Aquaporin 4 in rodent spinal cord: Relationship with astrocyte markers and chondroitin sulfate proteoglycans

Vitellaro‐Zuccarello

Mazzetti

Bosisio

et al. 2005

Glia

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Water balance between cells and extracellular compartments is essential for proper functioning of the central nervous system, as demonstrated by its perturbations in pathological conditions. Aquaporin 4 (AQP4) is the predominant water channel in brain and spinal cord, where it is present mainly on astrocytic endfeet contacting vessels. A role in water homeostasis control has been proposed also for the extracellular matrix, that in brain consists mainly of chondroitin sulfate proteoglycans (CSPGs). Using cytochemical and immunocytochemical techniques, we investigated their distribution in rodent spinal cord, to better understand the role of these two classes of molecules. The results show that in spinal gray matter AQP4 labeling is intense in all perivascular profiles and (1) displays a marked dorsoventral gradient in the neuropil; and (2) coexists extensively with glial glutamate transporter-1 (GLT-1) but scarcely with glial fibrillary acidic protein (GFAP). In white matter the overlap between AQP4, GLT-1, and GFAP is almost complete. Ultrastructural examination shows that AQP4-labeled astrocytic processes surround blood vessels, neuronal perikarya and processes, and both asymmetric and symmetric synapses, indicating that the protein may be involved in the regulation of water fluxes around both inhibitory and excitatory synapses. CSPGs, visualized by labeling with Wisteria floribunda agglutinin, show a distribution complementary to that of AQP4, being absent or weekly expressed in AQP4-enriched areas. These findings suggest that different mechanisms may contribute to the regulation of water homeostasis in different spinal cord regions.

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Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Cited by 23 publications

References 63 publications

Theca Interna: The Other Side of Bovine Follicular Atresia1

Theca Interna: The Other Side of Bovine Follicular Atresia1

Spatial and Temporal Pattern of Changes in the Number of GAD65-Immunoreactive Inhibitory Terminals in the Rat Superficial Dorsal Horn following Peripheral Nerve Injury

Distribution of Aquaporin 4 in rodent spinal cord: Relationship with astrocyte markers and chondroitin sulfate proteoglycans

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Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B4 on primary sensory neurones in seven mammalian species

Cited by 23 publications

References 63 publications

Theca Interna: The Other Side of Bovine Follicular Atresia1

Theca Interna: The Other Side of Bovine Follicular Atresia1

Spatial and Temporal Pattern of Changes in the Number of GAD65-Immunoreactive Inhibitory Terminals in the Rat Superficial Dorsal Horn following Peripheral Nerve Injury

Distribution of Aquaporin 4 in rodent spinal cord: Relationship with astrocyte markers and chondroitin sulfate proteoglycans

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Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species